Objective:To investigate the expression of human telomerase reverse transcriptase(hTERT)gene in salivary gland tumors and to study its clinicopathological significance.Methods:In situ- mRNA hybridization method was used to detect the expression of hTERT mRNA in 10 cases of normal salivary gland tissues, 30 of benign salivary gland tumors and 43 of malignant salivary gland tumors.The relationship between hTERT mRNA expression and sex,age, tumor size, location, histologic differentiation of the malignant tumors was analyzed by using ?2 test.Results:hTERT expression was negative in all normal tissues. Positive rate of hTERT mRNA expression was 3.3%(1/30) and 83.7%(36/43) in benign and malignant salivary gland tumors respectively(P0.05).Conclusion:hTERT mRNA expression may be used to distinguish malignant salivary gland tumors from benign.

0.05);LMP-1 and EBNA-2 in papillary thyroid carcinoma had no correlation.Conclusion Some thyroid papillary carcinomas may be related to EB virus infection,the relationship has yet to be further studied.

Objective To investigate the effects of sand therapy temperature on bone reconstruction and numerical study on the heat transfer.Methods CT scan imaging was performed respectively for four times(before and after OA model was set up,and after the first and second weeks during the sand therapy).Import the scan data to MIMICS software,and the changes of femoral bone mass layer were analyzed.After combining the muscle,femur,bone marrow and meshing,the established three-dimensional model of the STL format was introduced into the COMSOL software for heat transfer simulation and effect of stress on bone remodeling induced by temperature field.Results Changes of four CT scan data were analyzed.The soft bone volume was reduced,while the dense and hard bone volume were increased.Numerical simulation on the heat transfer showed the temperature distribution of the thigh and the femur.Conclusions The thermal stress produced by sand therapy temperature exert promoting effect on the femur bone remodeling.

Objective To explore the experimental condition for hepatocellular steatosis models of Changliver cell induced by o‐leic acid (lleic acid ,OA) .Methods Changliver cells were induced by different concentration of oleic acid for different periods .MTT was used to detect hepatic cell activity ,oil red 0 staining was used to observe intracellular lipid droplets acumulation ,glycerin 3 phosphate oxidase method was applied to detect the contents of triglyceride (TG) in the Changliver cell .Results Hepatocellular steatosis models of Changliver cell can be established successfully by 0 .2 mmol/L OA inducing for 24 hours .TG content in model cells was (379 .98 ± 23 .19)mg/g ,however ,it was (185 .03 ± 12 .68)mg/g in control cells ,the difference was statistically significant (P< 0 .01) .Conclusion The proper condition for establishing hepatocellular steatosis models is 0 .2 mmol/L OA inducing Changliver cells for 24 h .This model is the reliable choice for nonalcoholic fatty liver disease research .

Objective:To investigate the Effect of Pine muscle nursing training and external application of loose tendon ointment on muscle strength and muscle tension in patients with disused knee joint contracture.Method:From August 2018 to June 2019, 80 subjects were selected in the hospital, divided into three groups using the random number table method, and arranged in different wards. The routine rehabilitation group performs routine hospital rehabilitation (good limb placement, massage, active and passive exercise training), routine rehabilitation + pine muscle nursing training group is to implement rehabilitation nursing training on the basis of conventional rehabilitation treatment in hospital (relaxation training, hand micro-vibration therapy, pasteurization training), routine rehabilitation + pine muscle nursing training + medication group is based on the conventional rehabilitation treatment and pine muscle nursing training of hospitals to implement external application of loose tendon ointment. After 8 weeks of intervention, the muscle strength of the affected quadriceps (knee extensor) and the biceps femoris (Knee flexor) were compared.Results:Before the intervention, the patients in the three groups all experienced a decrease in the strength of the affected quadriceps muscle, and the flexion contracture of the knee joint caused by the hypertonic biceps muscle tension (minimum value ≥5uV) ( P>0.05). After 8 weeks of intervention, the muscular tone of the affected biceps femoris in the three groups was decreased ( F=10.623, P<0.001), and the muscle strength of the quadriceps femoris was increased ( F=10.888, P<0.001).The pairwise comparison results showed that compared with the conventional rehabilitation group, the difference between the conventional rehabilitation + pine muscle nursing training group was significant ( P<0.05), and the difference between the conventional rehabilitation + pine muscle nursing training + medication group was significant ( P<0.001). Compared with the routine rehabilitation + pine muscle training group, the difference between the conventional rehabilitation group and the routine rehabilitation + pine muscle nursing training + medication group was significant (both P<0.05). Conclusion:Patients with disused knee flexion contracture treated with Pine muscle nursing training combined with external application of loose tendon ointment can loosen the contraction and stiffness of the joint tissue, significantly reduce the muscle tension of the flexor knee muscle, increase the strength of the extensor knee muscle, restore and improve The rhythmic contraction and relaxation ability of the flexion and extension knee muscles makes their muscle strength/muscle tension ratio tend to balance, enhance the coordination control ability between muscle groups, and effectively reduce the degree of knee flexion contracture.

Legg-Calve-Perthes (LCP) disease is characterized by idiopathic avascular osteonecrosis of the epiphysis of the femur head. The main factor that plays a role in the etiology of the disease is decreased blood flow to the epiphysis. Many predisposing factors have been suggested in the etiology of LCP disease, and most have varying degrees of effects. Here we present the case of a boy aged 4 years and 10 months with complaints of short stature and a diagnosis of multiple hypophyseal hormone deficiency, in whom LCP disease and difficult birth-related pituitary stalk interruption syndrome were identified by anamnesis. The present case revealed that LCP disease and hypophyseal hormone deficiency could be secondary to difficult birth and that LCP disease could be secondary to insulin-like growth factor 1 deficiency. Additionally, to the best of our knowledge there is no published case on the relation between LCP disease and insulin-like growth factor 1 deficiency. Therefore, we believe that this case is worthy of presentation.
Causality ; Diagnosis ; Epiphyses ; Femur Head ; Humans ; Hypopituitarism* ; Legg-Calve-Perthes Disease* ; Male ; Osteonecrosis ; Parturition ; Pituitary Gland

Bone maintenance theory considers that the external load is the direct stimulating source of the bone remodeling. In this article, the method of experimental observation of self-repairing process of the bone defect and related results are introduced. Firstly, a hole was drilled in the rabbit thighbone so that the continuity of the bone was changed. Then bone defect model was established, and the thighbone data were obtained by using CT scanning, and the self-repairing process of bone defects caused by growth factor were observed and analyzed by MIMICS software. Finally, the relationship between volume changes of sclerotin was established, and scientific bases were provided for introducing the bionic topology optimization method to the remodeling process. The experimental results showed that the self-repairing of the each layer sclerotin of the young rabbits was faster than that of the adult ones under the same condition. In addition, the volume always changes contrarily between the spongy bone and enamel bone during the self-repairing process of bone defect.
Animals ; Bone Regeneration ; physiology ; Female ; Femoral Fractures ; physiopathology ; Femur ; injuries ; Fracture Healing ; physiology ; Male ; Osteogenesis ; physiology ; Rabbits

OBJECTIVETo prospectively compare of no drain or with drain after unilateral total knee arthroplasty (TKA).

METHODSA randomized prospective study was conducted between February 2006 and February 2007. The study included 100 patients who were divided into 2 groups: group A (n = 50), which included patients without drain release, and group B (n = 50), which included patients who had a drain release. The true total blood loss was calculated depending on the height, body mass and pre-and post-operative Hct, and hidden blood loss was gotten by subtracting the visible blood loss from total loss. The recovery after surgery, the correlative complications and transfusion amounts were compared and analyzed.

RESULTSAll 100 patients undergoing TKA were involved in the result analysis. In patients without a drain, the total blood loss was (535 +/- 295) ml and the hidden blood loss was (513 +/- 290) ml, in patients with a drain, the total blood loss was (853 +/- 331) ml and the hidden blood loss (689 +/- 324) ml. The total and hidden blood loss, blood transfusion amount between drain-group and without drain-group was significantly different (P < 0.05). The difference in the incidence of infection and post-op recovery and correlative complications did not reach statistical significance.

CONCLUSIONSIn patients having primary TKA, using a drain, compared with no use of a drain, does not show us a clear advantage that make us accept it. Without a drain release could present an effective and economic method for reducing blood loss and preventing blood transfusion in patients undergoing unilateral TKA.

Aged ; Arthroplasty, Replacement, Knee ; Drainage ; Female ; Humans ; Male ; Middle Aged ; Postoperative Hemorrhage ; prevention & control ; Prospective Studies