1.Expression of cyclooxygenase-2 mRNA and identification of its splice variant in human myometrium obtained from women in labor.
Yinping, HUANG ; Duyun, YE ; Ping, WU ; Yanjun, HUANG ; Li, ZHANG ; Xiaoyan, ZHOU ; Yunfeng, HUANG ; Ping, YUAN ; Daijuan, ZHANG ; Jingyuan, WAN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2005;25(1):5-7
In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing.
Amino Acid Sequence
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Base Sequence
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Cyclooxygenase 2/*biosynthesis
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Cyclooxygenase 2/genetics
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Labor Onset/*metabolism
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Molecular Sequence Data
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Myometrium/*enzymology
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Myometrium/metabolism
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RNA, Messenger/biosynthesis
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RNA, Messenger/genetics
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Sequence Analysis