1.Genotypic Detection of rpoB and katG Gene Mutations Associated with Rifampicin and Isoniazid Resistance in Mycobacterium Tuberculosis Isolates: A Local Scenario (Kelantan)
Nurul-Ain Ismail ; Mohd Fazli Ismaill ; Siti Suraiya Md Noor ; Siti Nazrina Camalxaman
Malaysian Journal of Medical Sciences 2016;23(1):22-26
Background: Drug resistant tuberculosis (DR-TB) remains a public health issue that is of major concern on a global scale. The characterisation of clinical isolates may provide key information regarding the underlying mechanisms of drug resistance, and helps to augment therapeutic options. This study aims to evaluate the frequency of gene mutations associated with Rifampicin (RIF) and Isoniazid (INH) resistance among nine clinical isolates. Methods: A total of nine drug resistant Mycobacterium tuberculosis clinical isolates were screened for genetic mutations in rpoB and katG using polymerase chain reaction (PCR) amplification and DNA sequencing. Genotypic analysis was performed to detect the mutations in the sequence of the target genes. Results: Our findings reveal that 80% of the isolates possess mutations at codon 119 (His119Tyr) and 135 (Arg135Trp and Ser135Leu) within the rpoB gene; and 70% possess mutations in the katG gene at codon 238 with amino acid change (Leu238Arg). Conclusion: Findings from this study provide an overview of the current situation of RIF and INH resistance in a hospital Universiti Sains Malaysia (HUSM) located in Kelantan, Malaysia, which could facilitate molecular-based detection methods of drug-resistant strains. Further information regarding the molecular mechanisms involved in resistance in RR-/MDR-TB should be addressed in the near future.
Mycobacterium tuberculosis
2.Differentiation between Mycobacterium bovis and Mycobacterium tuberculosis Infection.
Won Jung KOH ; Young Kil PARK ; Chang Ki KIM
Korean Journal of Medicine 2012;83(4):550-552
No abstract available.
Mycobacterium
;
Mycobacterium bovis
;
Mycobacterium tuberculosis
3.Interactions between ciprofloxacin and other antituberculous drugs in the growth inhibition of mycobacterium tuberculosis.
Sang Jae KIM ; In Soon KIM ; Kwan Suk PARK ; In Hee SONG ; Woo Hyun CHANG ; Suk JEONG
Journal of the Korean Society for Microbiology 1991;26(2):147-158
No abstract available.
Ciprofloxacin*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
4.Efficiency of different primers in polymerase chain reacion to detect mycobacterium tuberculosis in clinical specimens.
Myung Sup SHIM ; Sung Youn LEE ; Sang Hyun CHO ; Young Kil PARK ; Gil Han BAI ; Sang Jae KIM
Journal of the Korean Society for Microbiology 1993;28(5):391-395
No abstract available.
Mycobacterium tuberculosis*
;
Mycobacterium*
5.Primer directed amplification of mycobacterium tuberculosis DNA in clinical specimens I. primers and reaction conditions.
Sang Jae KIM ; Young Kil PARK ; Sang Hyun CHO ; Myung Sup SHIM
Journal of the Korean Society for Microbiology 1992;27(1):35-44
No abstract available.
DNA*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
6.Primer directed amplification of mycobacterium tuberculosis DNA in clinical specimens I. primers and reaction conditions.
Sang Jae KIM ; Young Kil PARK ; Sang Hyun CHO ; Myung Sup SHIM
Journal of the Korean Society for Microbiology 1992;27(1):35-44
No abstract available.
DNA*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
7.Purification and immunochemical charaterization of alpha-antigen from the culture filtrate of mycobacterium tuberculosis.
Seok Kwun KIM ; Tae Hyun PAIK ; Hwa Jung KIM ; Jeong Kyu PARK ; Tae Kyung CHOI
Journal of the Korean Society for Microbiology 1991;26(1):45-60
No abstract available.
Mycobacterium tuberculosis*
;
Mycobacterium*
8.Spoligotyping technique: improvement and application in classification Mycobacterium tuberculosis
Lien Thi Kim Vu ; Au Thi Hai Tran ; Khanh Van Tran ; Nga Thi Quynh Do ; Hung Van Nguyen ; Dung Thi Thu Nguyen ; Trao Tan Vu ; Ulf R.Dalhe
Journal of Preventive Medicine 2007;17(6):23-29
Background: There are many methods used in epidemiological studies of tuberculosis (TB) bacteria but Spoligotyping method is widely used with high accuracy, simple procedure, and carried out on strains containing a little of IS6110 segment \r\n', u'Objectives: To improve Spoligotyping technique and apply this technique to classify Mycobacterium tuberculosis\r\n', u'Subjects and method: Subjects and methods: The study included 12 medical waste samples collected at Thai Binh Hospital of Tuberculosis and 19 samples obtained from the The Hanoi Institute for Tuberculosis and Lung Diseases. Spoligo model of 31 samples were analyzed based on Spotclust and SpoIDB4 database and divided up into family and subfamily.\r\n', u'Results: Spoligotyping technique has good results with the PCR product amplified 40 cycles and presented the film in 18 hours. Obtained results in 31 medical waste samples belong to 4 families: Beijing, EAI, T1 and H3-LAM9. EAI and Beijing are dominant families with 45.16% and 38.7%), respectively. T1 and H3-LAM9 are 12.9% and 3.22%, respectively. The number of samples in the study is little but the obtained rate of different spoligo models of strains are quite diversified (41.9%)\r\n', u'Conclusion: This result is relatively appropriate with previous studies on the distribution of EAI and Beijing families in Vietnam and the world. Spligotyping technique distinguished samples belonging to Beijing or non-Beijing families, which support for clinical treatment and development of new vaccines.\r\n', u'\r\n', u'\r\n', u'
Mycobacterium tuberculosis
;
Tuberculosis
9.A ten-year retrospective study on the clinical features and outcomes of Pediatric Intestinal Tuberculosis admitted in UP-Philippine General Hospital
Eleonor G. Rodenas-Sabico ; Germana Emerita V. Gregorio ; Ma. Liza Antoinette M. Gonzales
Acta Medica Philippina 2020;54(2):128-133
Objective:
To describe the clinical, biochemical, microbiologic, radiologic and histological features and outcome of intestinal TB.
Methods:
Medical records of patients diagnosed with intestinal TB were reviewed. Cases were considered bacteriologically-confirmed if intestinal tissue was positive on smear culture or polymerase chain reaction (PCR); and clinically-diagnosed if with clinical, histologic, and radiologic evidence of extra-pulmonary TB.
Results:
Fifteen patients [Mean (SD) age: 13 (4) years; 53% females] were included. One was bacteriologically-confirmed; and fourteen were clinically-diagnosed. Fever (87%) and abdominal pain (73%) were commonly seen. Seven (47%) had anemia, 5 (33%) leukocytosis and 10 (71%) hypoalbuminemia. Eleven (73%) were positive on smear or TB PCR of various specimens. Nine of 10 (90%) patients with an abdominal CT scan had thickening of bowel loops. Three with intestinal biopsy demonstrated caseation granuloma. Fourteen patients were given quadruple anti-TB medications. Six had surgery, 8 had no disease-related complications, 4 died of sepsis and 3 were lost to follow up.
Conclusion
Intestinal TB presents with non-specific clinical and laboratory features. Radiologic findings may provide a clue to the diagnosis. Histologic confirmation in intestinal tissue was only seen in a few cases. The prognosis was favorable for patients who completed the anti-TB treatment.
Tuberculosis, Extrapulmonary
;
Mycobacterium tuberculosis
10.Mycobacterium Tuberculosis in Spinal Tuberculosis.
Myung Sang MOON ; Sung Sim KIM ; Han Lim MOON ; Dong Hyeon KIM
Asian Spine Journal 2017;11(1):138-149
Even in an era of remarkable medical advances, there is an issue of why tuberculosis remains in the list of disastrous diseases, afflicting humans and causing suffering. There has not been a plausible answer to this, and it has been suggested that clinicians and medical scientists could presently not win the war against the tubercle bacilli. With regards to this issue, based on the authors' own clinical and research experiences, in this review, the available literature was revisited in order to address the raised questions and to provide recent information on characteristics of tubercle bacilli and possible ways to more effectively treat tuberculosis.
Humans
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Tuberculosis
;
Tuberculosis, Spinal*