Background: In traditional medicine, Jur Ur-4 Tang, which is widely used for the treatment of diabetes, has been technologically processed and transformed into a modern pharmaceutical form as soluble granules. Therefore, it is essential to evaluate the pharmacological effects of the soluble granules derived from Jur Ur-4 Tang. Aim: To study the effect of granules extracted from Jur ur-4 Tang in a model of type 2 diabetes mellitus in experimental rats. : Materials and Methods : T2DM in rats was induced by a high-fat diet and a low-dose streptozotocin (STZ 35 mg/kg). Then, oral gavage administration of three different doses of Granule (450 mg/kg, 900 mg/kg) was given to T2DM rats. Experimental results showed that Granule dramatically reduced the levels of fasting blood glucose, fasting blood insulin, and GLUT4. Results:: In the glucose tolerance test, granules at doses of 450 and 900 mg/ kg significantly reduced blood glucose levels at 60 and 120 minutes (p < 0.01). Additionally, the soluble granules at all tested doses significantly decreased blood insulin levels and increased GLUT4 expression (p<0.01). Conclusion The granules lower blood glucose levels.

Introduction: One of the main ingredients of Anar-5 tablets is Piper longium L. Piperine alkaloids are the main active ingredients of the Piper longum and have anti-inflammatory, antioxidant and gastric protection properties.In the framework of the standardization study of Anar-5 tablets, a method was developed to determine the content of piperine in highly perpormance liquid chromatography, and then it was sought to include it in the method of analysis of Anar-5 drugs. Goal: Quantitative determination of piperine in Anar-5 tablets and validate the method Material and Methods: The research was conducted in the Chemistry and Chemical Technology Laboratory of the Research Center of the Institute of Traditional Medicine and Technology. And Anar-5 tablets (serial number 04012020) that are produced for experimental were used in the research. The standard substance, piperine alkaloids, was purchased from Green Chemistry.Purification of HPLC (organic solvent methanol, 99.9%, distilled water) was used. The EX 1600 HP/ PUMP high-performance liquid chromatography instrument (column Arcus EP+-C18, 5µm, 4.6x250 mm) and the organic solvent filter 0.45 μm syringe filter were used. The methodology related to this research was discussed and approved at the online meeting of the Ethics Committee of the Academy of Sciences on January 26, 2021. SPSS 16 software was used to statistically program the survey results. Results : According to the above method, the retention time of the standard piperine is 10.38± 0.02 minutes, and the retention time of the piperine in Anar-5 tablets is 10.42±0.033 minutes. Relative velocity deviation RSD 1.077%, accuracy 0.65446±0.0068mg, stability 0.61298±0.013mg, capture time 10.42±0.033 minutes, relative standard deviation RSD≤2%, specificity 10.35 minutes, The equation of a line constructed with a standard curve is y=43360x-33587 and the correlation coefficient R2=0.9989. The piperine content of Anar-5 tablets was determined to be 0.61298±0.013 mg. The LOD and LOQ for piperine were in 2.268 μg/ml and 6.873 μg/ml, respectively. Conclusion The content of piperine in Anar-5 tablets can be determined by the HPLC method, and the appropriate conditions for this method have been established. The HPLC method is unique, accurate, linear, and stable, and meets ICH Q2 (R1) guideline criteria.

: The “Zidraga-6” prescription, which is widely used in traditional medicine, was selected and technologically developed, and formed into capsule medicine. Zidraga-6 capsule was prepared using six different herbal Capsicum annuum L. Holarrhena antidysenterica Wall ex, Atragene sibirica L, Embelia ribes Burm, Terminalia chebula Retz, and Kaempferia galanga L. Goal: This study was conducted to standardize and determine the safety and quality parameters of the Zidraga-6 capsule. Materials and Methods: Quality (moisture, total ash) and safety parameters were determined by Mongolian National First Pharmacopoeia methods. The content of the main biologically active compounds in the Zidraga-6 capsule was identified using TLC. In addition, the content of biologically active compounds is determined by UV spectrophotometer methods. The results were processed using basic biostatistical methods, calculating the arithmetic mean (M), standard deviation (δ), and standard error (m) using the SPSS 20.0 program. The ethical approval for the study was obtained by a decision of the Ministry of Health - Medical Ethics Review Committee on research on May 23, 2023 (23/029). Results: The presence of p-methoxycinnamate, gallic acid, oleanolic acid, and capsaicin in the Zidraga-6 capsule drug was detected by the TLC method, and their Rf values were 0.26, 0.24, 0.82, and 0.45, respectively. The average weight of Zidraga-6 capsules was 0.4±0.019 g, moisture was 9.63±0.09%, disintegration was 6.48±0.37 minutes, dissolution was 94.68±2.85%. The content of methods biologically active compounds in the Zidarga-6 capsule was determined total triterpene saponins as 1.89±0.19% by UV spectrophotometric methods. Conclusion We determined quality and safety parameters of the Zidraga-6 capsule were defined and the Mongolian National Pharmacopoeia monograph’s draft for the Zidraga-6 capsule was developed.