1.The Outcome of the Seminal Fluid Parameters Collected via Coitus Interruptus versus Masturbation
M Kamarul Bahyah ; Z Ahmad Murad ; I Ghazali ; R Roszaman ; A W Noraziana ; A Mokhtar ; M H Omar
The Medical Journal of Malaysia 2010;65(1):23-26
A one year study was carried out to determine the outcome
of the seminal fluid parameters collected via masturbation
and coitus interruptus in 151 patients who were undergoing
intrauterine insemination (IUI) and patients who came for
seminal analysis. There were no statistically significant
differences in terms of volume, concentration, progressive
motility and normal morphology from specimens collected
via coitus interruptus compared to specimens collected via
masturbation. Pregnancy outcomes were also comparable.
2.Fagonia cretica: Identification of compounds in bioactive gradient high performance liquid chromatography fractions against multidrug resistant human gut pathogens
Tabassum, T. ; Rahman, H. ; Tawab, A. ; Murad, W. ; Hameed, H. ; Shah, S.A.R. ; Alzahrani, K.J. ; Banjer, H.J. ; Alshiekheid, M.A.
Tropical Biomedicine 2022;39(No.2):185-190
Plants are alternative source of natural medicines due to secondary active metabolites. Fagonia cretica
extracts and Gradient High-Pressure Liquid Chromatography fractionations were checked against
multidrug-resistant gastrointestinal pathogens including, Salmonella typhi, Escherichia coli and Shigella
flexneri. ESI-MS/MS analysis of bioactive HPLC fractions was performed to elucidate antibacterial
compounds. F. cretica extracts exhibited potential antibacterial activity. Twenty-four (24) HPLC fractions
were obtained from methanol, ethanol and aqueous extracts of F. cretica. Eighteen (18) fractions showed
antibacterial activity, while no activity was observed by the remaining six (6) fractions. HPLC fractions,
F1 (25g ± 0.20 mm) and F2 (15f
± 0.12 mm) of aqueous extract exhibited activity against multidrug
resistant GI pathogens. Gallic acid, quinic acid, cyclo-l-leu-l-pro, vidalenolone, liquirtigenin, rosmarinic
acid and cerebronic acid were identified in F1 fraction of aqueous extract, while succinic acid, cyclo (l-Leul-Pro) and liquirtigenin were identified in F2 fraction of aqueous extract through ESI-MS/MS analysis.
F. cretica extracts and HPLC fractions showed potential activity against MDR GI pathogens. Vidalenolone,
Cyclo-1-leu-1-pro and Cerebronic acid are first time reported in F. cretica. Further characterization of
bioactive compounds from F. cretica may be helpful to elucidate antibacterial therapeutic molecules.