1.Determination of Stilbence Glucoside in Polygonum Multiflorum by HPLC
Ting LAN ; Baolong WANG ; Minghua LI ; Muqiong LI ; Wei HE
China Pharmacist 2014;(10):1774-1775
Objective:To determine the content of stilbene glucoside in polygonum multiflorum by HPLC. Methods: An Agilent Eclipse Plus C18(100 mm ×4.6 mm,3.5 μm)column was adopted with acetonitrile-water(18∶82) as the mobile phase at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 320nm and the injection volume was 8 μl. Results:The linear range of stilbene glucoside was 38-237. 5 μg·ml-1(r=0. 999 4)with the average recovery of 98. 32%(RSD=1. 59%). Conclusion: The method is simple, sensitive and specific in the content determination of stilbene glucoside in polygonum multiflorum.
2.Effects of four decalcifying solutions on pathological structure of bone and bone marrow tissues
Canqiao LUO ; Muqiong MO ; Zhaoming NIE ; Juemin ZHONG ; Yubin LI
Chinese Journal of Tissue Engineering Research 2015;(37):5928-5932
BACKGROUND:As the bone and marrow tissue have very special structure, it is difficult to simultaneously display the bone with tough hard tissue and bone marrow tissues containing various immature hematopoietic cel s in the conventional process of pathological section preparation. OBJECTIVE:To choose the best decalcifying solution that cannot only completely remove the calcium in the bone tissue but also protect the structure of bone marrow tissues and cel s from damage. METHODS:Bone marrow tissues from the long bone of dogs were randomly divided into four groups. Under the same conditions, the bone marrow tissues were decalcified with 14%formaldehyde saline solution of nitric acid (group A), 14%nitric acid solution (group B), 20%A saline solution of hydrochloric acid formaldehyde (group C) and 20%A hydrochloric acid aqueous solution (group D). Decalcified time was recorded, fol owed by routine dehydration, section, hematoxylin-eosin staining and microscopic observation. Pathological section quality and hematoxylin-eosin staining were compared among the four groups. RESULTS AND CONCLUSION:Group A had the best sections and hematoxylin-eosin staining, strongest decalcified ability, shortest decalcified time and minimum damage to the bone marrow. Group B had the worst results of section and hematoxylin-eosin staining, in which, the bone tissues were loose and became yel ow and the bone marrow tissue were damaged greatly, and the decalcified effect was worse. Group C was worse than group A in decalcified ability, damage degree, section quality and hematoxylin-eosin staining results. Group D also had a better result of section and hematoxylin-eosin staining as wel as exhibited uniform decalcification effect and less damage to the bone marrow, which was ranked between group B and group C. Al the four kinds of decalcifying solutions have a good decalcification ability, but the section quality and hematoxylin-eosin staining results rank as fol ows:Group A>Group C>Group D>Group B. Taken together, 14%formaldehyde saline solution of nitric acid is ideal for the clinical preparation of pathological sections.
3.Study on Acute Toxicity of Xiaobai Capsules in Mice
Yangyang SONG ; Ting LAN ; Minghua LI ; Xiaoye LI ; Yanguo ZHANG ; Muqiong LI
China Pharmacist 2016;19(7):1411-1412
Objective: To study the acute toxicity of Xiaobai capsules in mice after intragastric administration .Methods: The mice were randomly divided into two groups , the treatment group and the control group .The treatment group was given Xiaobai cap-sules by gavage, 3 times daily.The acute toxicity was recorded, and the median lethal dose (LD50) and the maximum dose were deter-mined.Results:The maximum daily dose of Xiaobai capsules was 141.6 g· kg-1(equivalent to 211.3 times of the clinical dose).At the dose, the mice showed no toxicity without death in 14 days or changes in organs after the dissection .Conclusion:Xiaobai capsules have very low acute toxicity in mice after intragastric administration with high security .