Traditional medicine describes common flu as excessof blood and bile heat combined with microbes which results infectious heat. Deva-5is a decoction used which treats infectious heat. The aim of this study is to analyze components of Deva-5 decoction by traditional methods. Deva- 5 is composed of Gentianadecumbens L., Terminalia Chebula Retz., Polygonium bistorta L., Momordica cochinchinensis (Lour) and Chiazospermum erectum Bernh.It has a cool power and dispels infectious heat andkills microbes. The main component of the decoctionis Gentiana decumbens L. Ithas a sweat taste and clears heat, kills microbes, and cures typhus. Medical practitioners of Mongolia, Tibet, and Tuva use Gentiana decumbens L.as a substitution to deva (sngo-de-ba).It has a bitter taste and a cool power. It also has antimicrobial and heat clearing effects. All components of Deva-5 decoction have a cool power and the decoction is compounded by power.

Antinociceptive effect of Deva-5 (Gentiana decumbens L., Terminalia Chebula Retz., Polygonium bistorta L., Momordica cochinchinensis Lour., Chiazospermum erectum Bernh) used in traditional medicine for treatment of infectious heat disease was examined in both chemically- and temperature-induced pain models of mice. Deva-5 administered orally at 100 and 200 mg/kg significantly reduced the writhings induced by acetic acid from control values of 37.7±6.7 (N = 6) to 24.5±3.9 (N = 6) and 18,8±2,8 (N = 6), respectively (p<0.05). When administered at 100 and 200 mg/kg, Deva-5 reduced the paw licking time for the second phase of the formalin test from the control value of 92.6±12.9 s (N = 6) to 69.8±5.7 s (N = 6) and 70.1±11.7 s (N = 6), respectively (p<0.05). Deva-5 had no significant effect on the first phase of the formalin test. As evaluated by the tail immersion test, Deva-5 at doses of 100 and 200 mg/kg, showed no analgesic activity. These results show that Deva-5 has antinociceptive activity and suggest that this effect is probably an indirect anti-inflammatory effect, which does not involve the central nervous system.

Traditional medicine describes common flu as excess of blood and bile heat combined with microbes which results infectious heat. Deva-5 is a decoction used which treats infectious heat. The aim of this study is to analyze components of Deva-5 decoction by traditional methods. Deva-5 is composed of Gentiana decumbens L., Terminalia Chebula Retz., Polygonium bistorta L., Momordica cochinchinensis (Lour) and Chiazospermum erectum Bernh. It has a cool power and dispels infectious heat and kills microbes. The main component of the decoction is Gentiana decumbens L. It has a sweat taste and clears heat, kills microbes, and cures typhus. The effects of water extract of Deva-5 on pathogenic bacteria including Streptococcus pneumonia, Staphylococus aureus, and Escherichia coli were examined. Plates of bacteria treated with Deva-5 at 6 different concentrations (1 -30 %) were incubated at 37°C for 24 h and then the diameter of the circle of bacterial growth was measured. Amoxicillin, nistatin, flukanosol, and ciprofloxacin were used as comparison. Acute and chronic toxicities of Deva-5 were determined in mice. Deva-5 was given orally to mice at doses of 200 mg/kg and 500 mg/kg for 28 days. In addition, therapeutic dose of Deva-5 was also determined in mice. Deva-5 inhibited growths of Streptococcus pneumonia and Staphylococus aureus at concentrations of 5 %, 10 %, 20 %, and 30 %. Deva-5 more effectively inhibited growth of Streptococcus pneumonia than that of Staphylococus aureus. Growth of Escherichia coli was not inhibited by Deva-5 treatment. The median lethal dose of (LD50) of Deva-5 was 6.89 g/kg. There were no difference between the control and Deva-5-treated groups in movements, hair color, food consumption, and the number of offspring of mice. No histopathological changes were observed in solid organs of animals treated with Deva-5. Therapeutic doses of Deva-5 were found to be 50-150 mg/kg. These results suggest that Deva-5 has antibacterial effects in vitro and it is safe in mice.