1.Demonstration of Specific IgM Antibodies to Treponema pallidum in Human Syphilis and Their Decrease after Penicillin Treatment.
Min Geol LEE ; Ferdinand MULLER
Korean Journal of Dermatology 1985;23(6):752-758
No abstract available.
Antibodies*
;
Humans*
;
Immunoglobulin M*
;
Penicillins*
;
Syphilis*
;
Treponema pallidum*
;
Treponema*
2.Biomechanical measurement system for measuring frictional properties of synovial joint after cartilage injury
Yiping MU ; Muller OTTO ; Wullker NICOLAS
Chinese Journal of Tissue Engineering Research 2013;(26):4833-4840
10.3969/j.issn.2095-4344.2013.26.012
3.Ability of endophytic fungi isolated from Nepenthes ampullaria to degrade polyurethane
Shirley Bong Wuan Lii ; Changi Wong ; Muller
Malaysian Journal of Microbiology 2017;13(3):172-179
Aims: Waste electric and electronic equipment (WEEE) are among the fastest growing waste products worldwide and
solutions to their remediation are urgently needed. Bioremediation is a green approach that is helpful to minimize
environmental pollution associated with Electronic waste (E-waste). The present study aimed at exploring the potential
of endophytic fungi from Nepenthes ampullaria for bioremediation purposes of the plastic component in E-waste,
polyurethane (PUR) polymers.
Methodology and results: Endophytic fungal isolates were assessed for their ability to degrade PUR as well as their
ability to utilise PUR as sole carbon source. Nine (9) out of 150 isolates demonstrated the ability to efficiently degrade
polyurethane in solid medium and the top three (3) isolates were able to grow on PUR as the only carbon source. These
three isolates were identified using ITS1 and ITS4 and found to be closely related to the genus Pestalotiopsis. The top
two of the three isolates were then assessed for their esterase enzyme activity as well as changes in their proteome
when grown with and without PUR. The highest enzymatic activity was found to be 1850.4 U/mL when tested using pnitrophenol
acetate as the substrate. Analyses of the 2-dimensional electrophoresis profile revealed changes in the
abundance of proteins when treated with polyurethane.
Conclusion, significance and impact of study: This study is to our knowledge the first on endophytes isolated from N.
ampullaria that can degrade PUR, and also their proteomes. Results obtained from this study can in the future help to
reduce polyurethane wastes. Besides degrading PUR polymer, endophytic fungi produce potential valuable proteins that
may find broad applications in bioremediation applications.
4.Aedes aegypti disregard humidity-related conditions with adequate nutrition
Canyon, D.V. ; Muller, R. ; Hii, J.L.K.
Tropical Biomedicine 2013;30(1):1-8
Weather variations have clear associations with the epidemiology of dengue fever
and populations of Aedes aegypti mosquitoes. Data on humidity associations, however, lags
with respect to its effect on host-biting, nectar-seeking and survival. This experimental study
on Ae. aegypti, sourced from the arid tropics, investigated the effect of low and high relative
humidity and diet in relation to host-biting, temporal variations in feeding frequency, and
mosquito mortality. In each environmental setting, 10 replicates, containing one male and
five female mosquitoes, were challenged with different nutritional sources every six hours
over 12 days. Results showed that host-biting did not diminish in low humidity and was six
times higher than expected. Sucrose feeding was observed to significantly moderate hostbiting
and water alone was inadequate for survival. The high host-biting rates help to explain
the intensity of dengue epidemics, while the ability of the mosquito to disregard adverse
humidity-related conditions helps to explain how dengue epidemics in arid tropical regions
can be just as devastating as those in the wet tropics.
5.New Developments in the Immunological Understanding and of Serodiagnosis in Syphilis.
Yonsei Medical Journal 1985;26(1):18-23
There are many serologic tests for syphilis. By means of the usual serologic tests, it is not possible to differentiate between patients who need therapy and those who are cured. In this paper I want to discuss the scientific developments and demonstrate the results of immunologic research in syphilis, which makes it possible to differentiate between treated and untreated cases.
Antibodies, Bacterial/analysis
;
Chromatography, Gel
;
Electrophoresis, Starch Gel
;
Fluorescent Antibody Technique
;
Hemagglutination Tests
;
Human
;
Immunoglobulin G/analysis
;
Immunoglobulin M/analysis
;
Syphilis/immunology*
;
Syphilis Serodiagnosis*
;
Treponema pallidum/immunology
6.'A life threatening scratch on little toe' - at most clinical suspicion the essential key in management of snake bite.
George PETER ; Panicker I GEORGY
Asian Pacific Journal of Tropical Biomedicine 2012;2(2):163-165
Snake bites are one among the under reported clinical emergencies from tropical countries. There are variations in clinical presentation of snake bites and its toxic features differ with the species and type of bite. There are lots of controversies in the treatment guidelines which often makes it difficult to manage. We report the case of a severe hemotoxic snake bite who presented to the outpatient service of our hospital with a trivial foot injury. Even though snakebites are familiar clinical situations for an emergency physician from tropics, we report this case as most are under reported. We also intend to emphasize the excellent outcome of appropriately diagnosed and treated cases of snake bite.
Adult
;
Animals
;
Anticoagulants
;
toxicity
;
Antivenins
;
therapeutic use
;
Blood Coagulation
;
Humans
;
Male
;
Snake Bites
;
blood
;
drug therapy
;
pathology
;
Snake Venoms
;
toxicity
;
Viperidae
7.Ixora coccinea Linn.: traditional uses, phytochemistry and pharmacology.
Manjeshwar Shrinath BALIGA ; Poruthukaran John KURIAN
Chinese journal of integrative medicine 2012;18(1):72-79
Ixora coccinea Linn., (Rubiaceae) commonly known as jungle of geranium and red ixora, is an evergreen shrub found throughout India. Depending on the medical condition, the flowers, leaves, roots, and the stem are used to treat various ailments in the Indian traditional system of medicine, the Ayurveda, and also in various folk medicines. The fruits, when fully ripe, are used as a dietary source. Phytochemical studies indicate that the plant contains important phytochemicals such as lupeol, ursolic acid, oleanolic acid, sitosterol, rutin, lecocyanadin, anthocyanins, proanthocyanidins, glycosides of kaempferol and quercetin. Pharmacological studies suggest that the plant possesses antioxidative, antibacterial, gastroprotective, hepatoprotective, antidiarrhoeal, antinociceptive, antimutagenic, antineoplastic and chemopreventive effects, thus lending scientific support to the plant's ethnomedicinal uses. In the present review, efforts are made in addressing its ethnomedicinal uses, chemical constituents, and validated pharmacological observations.
Animals
;
Humans
;
Medicine, Traditional
;
Phytotherapy
;
Plant Extracts
;
chemistry
;
pharmacology
;
Reproducibility of Results
;
Rubiaceae
;
chemistry
8.Screening of endophytic fungi for biofuel feedstock production using palm oil mill effluent as a carbon source
Moritz Muller ; Edwin Sia Sien Aun ; Joanne Yeo Suan Hui ; Julie Wong Wei Ming ; Jenny Choo Cheng Yi ; Changi Wong
Malaysian Journal of Microbiology 2017;13(3):203-209
Aims: Palm oil mill effluent (POME) is a major agricultural waste product of Malaysia. The aim of this study was to
identify endophytic fungi capable of producing biofuel feedstock utilizing POME.
Methodology and results: Endophytes were isolated from the Nipah palm tree, Nypa fruticans, and exposed to
different POME concentrations (25%, 50% and 75%), with and without the addition of nutrients. The utilization of glucose
was measured using the Dinitrosalicylic Acid assay whereas the lipid content in the fungal cells was extracted using the
Bligh and Dyer method with slight modifications. Three endophytic fungi that displayed the highest growth on POME
were identified using ITS 1 and 4 primers and found to be related to Pestalotiopsis sp., Lasiodiplodia theobromae and
Rhizoctonia bataticola. Nutrient addition caused an average increase of 8 times in biomass, indicating nitrogen
requirement for cell proliferation. The highest POME concentration (75%) resulted in lower biomass yield. Furthermore,
all fungal samples in high POME concentration and nutrient conditions showed a decrease in lipids accumulated per
milligram of biomass whereby lipid synthesis was enhanced under nitrogen limitation (25% without nutrients).
Conclusion, significance and impact of study: In conclusion, all fungal samples can be classified as oleaginous
microorganisms with Pestalotipsis sp. being the most efficient (up to 70% of its biomass). This is to our knowledge the
first study that shows the potential use of Pestalotiopsis sp., L. theobromae and R. bataticola for the utilisation of POME
as biofuel feedstock and could in the future potentially provide an alternative approach to the treatment of POME with
value-added effect.
9.What’s new in breast pathology 2022: WHO 5th edition and biomarker updates
Kristen MULLER ; Julie M. JORNS ; Gary TOZBIKIAN
Journal of Pathology and Translational Medicine 2022;56(3):170-171
The 5th edition WHO Classification of Breast Tumours (2019) has introduced changes to our practices. Highlights are presented below, with a focus on modifications to morphological subtype categorization. In addition, we summarize important updates to ER and PR testing made in the 2020 ASCO/CAP guidelines, and briefly discuss PD-L1 and Ki-67 testing in breast cancer.
10.Testicular sperm extraction in azoospermic patients with gonadal germ cell tumors prior to chemotherapy--a new therapy option.
Mark SCHRADER ; Markus MULLER ; Bernd STRAUB ; Kurt MILLER
Asian Journal of Andrology 2002;4(1):9-15
BACKGROUNDIn view of the high cure rates in patients with testicular germ cell tumors (TGCT), increasing clinical importance is attached to protection of fertility. Long-term infertility due to cytostatic therapy may be expected in more than 50% of the patients at a cumulative dose of cisplatin > 0.6 g/m2. The standard procedure for fertility protection in cancer patients includes cryopreservation of ejaculated spermatozoa. Considering that some patients have tumor-induced azoospermia, we examined the usefulness of testicular sperm extraction before therapy.
METHODA survey of the literature served as a basis for investigating biological and clinical aspects of the impact of chemotherapy on male fertility. A study of our patient population also enabled us to explore the option of extracting sperm from the contralateral healthy testis prior to treatment in 14 azoospermic patients with testicular germ cell tumors.
RESULTSWe were able to successfully recover haploid germ cells in 6/14 testicular biopsies from azoospermic patients with testicular germ cell cancer prior to treatment. Maturation arrest was found in 3/14 cases and Sertoli-cell-only syndrome in the rest. None of the patients had secondary healing or a treatment delay because of the testicular biopsy.
CONCLUSIONSince the post-therapeutic fertility status is difficult to predict in cancer patients, we think that TESE should be regarded as a general option prior to cancer treatment and offered to azoospermic cancer patients. New guidelines should be established in this connection.
Antineoplastic Agents ; adverse effects ; Germinoma ; complications ; drug therapy ; pathology ; Humans ; Infertility, Male ; chemically induced ; etiology ; therapy ; Male ; Oligospermia ; pathology ; Spermatozoa ; cytology ; drug effects ; Testicular Neoplasms ; complications ; drug therapy ; pathology