1.Molecular characterization of ascaris from Tibetan pigs by three mitochondrial markers of nad1, cox1 and cox2
Luo, H.Q. ; Zhang, H. ; Li, K. ; Lan, Y.F. ; Wang, X.Q. ; Khalid, M. ; Mujeeb, R. ; Huang, S.C. ; Li, J.K.
Tropical Biomedicine 2017;34(3):576-582
Ascaris is a helminthic parasite, which infects a wide range of host species
causing ascariasis, a predominant disease worldwide. This parasite causes significant economic
losses to the pig industry. The current study was designed to determine the Ascaris nematode
by the genetic characterization of three mitochondrial (mt) genes namely NADH dehydrogenase
subunit 1 (nad1), cytochrome oxidase subunit 1 (cox1) and cytochrome oxidase subunit 2
(cox2). A high infection rate of Ascaris nematode has been found in Tibetan pigs at the
slaughter houses in Tibet Autonomous Region of China. The nad1, cox1 and cox2 genes
sequences collected from adult Ascaris individuals were amplified by polymerase chain
reaction. The cloned-amplicons and the positive products were sequenced and phylogenetic
analysis was performed. The results indicated that the Ascaris infecting the Tibetan pigs
were Ascaris suum (A. suum). This is the first report on the isolation, identification and
genetic characterization of three mitochondrial genomes (nad1, cox1, and cox2) of A. suum
originated from Tibetan pigs at high altitudes in Tibet.
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