1.Antioxidant potential of phenolic extracts of Mimusops elengi
Shahwar Durre ; Raza Asam Muhammad
Asian Pacific Journal of Tropical Biomedicine 2012;(7):547-550
Objective:To evaluate the antioxidant potential of the phenolic extracts of Mimusops elengi (M. elengi) L. (Sapotaceae). Methods:The extract of stem bark and seeds of M. elengi were prepared in methanol and acetone:water (7:3). The acetone: water was further partitioned with ethyl acetate and n-butanol. Antioxidant activity of the extracts and partitioned fractions of M. elengi was evaluated in terms of radical scavenging potential (DPPH), inhibition of lipid peroxidation [ferric thiocyanate (FTC)], and total antioxidant activity (phosphomolybdate method). Total phenolics content were calculated using Folin-Ciocalteu reagent. Results:The stem bark extract partitioned with ethyl acetate exhibited highest amount of total phenols (98.0 mg GAE/g dry weight), among all other extracts, with 92.0%DPPH radical scavenging activity at concentration of 0.5 mg/mL, while methanol extract (stem bark) had maximum inhibition of lipid peroxidation (62.0%) and total antioxidant activity (771.0 mg/g GAE/g). A positive correlation occurred between total phenols and radical scavenging activity (R2= 0.922 9) and total antioxidant activity (R2= 0.945 1). Conclusions: Our study suggested that antioxidant activity of stembark extract of M. elengi is due the presence of phenolic compounds. Furthermore, the bark extract is a valuable source of natural antioxidants.
2.Epidemiological survey of helminths of goats in southern Punjab, Pakistan
Muhammad Mazhar Ayaz ; Muhammad Asif Raza ; Saeed Murtaza ; ; Saleem Akhtar
Tropical Biomedicine 2013;30(1):62-71
The main purpose of this study was to investigate the prevalence of helminths of goats such as Fasciola hepatica, Haemonchus contortus, Paramphistomum cervi,
Oesophagostomum columbian, Cotylophoron cotylophorum, Monezia expansa, Oestertagia oestertagi and Oestertagia circumcincta. The overall prevalence of all species of helminthes was 52% in goat. The study was designed to investigate the factors of helminths prevalence
on the basis of sex and age of goat with the help of Chi-square. All the results obtained were
non-significant due to some factors which directly affects the prevalence of helminths.
3.Anatomical Variations Of Cystic Artery: Telescopic Facts
Muhammad Zubair ; Lubna Habib ; Masoom Raza Mirza ; Muhammad Ali Channa ; Mahmood Yousuf ; Muhammad Saeed Quraishy
The Medical Journal of Malaysia 2012;67(5):494-496
The introduction of laparoscopic cholecystectomy has
stimulated a renewed interest in the anatomy of Calot’s
triangle 1. This triangle is a focal area of anatomical
importance in cholecystectomy and a good knowledge of its
anatomy is essential for both open and laparoscopic
cholecystectomy 2, 3. This triangle was described by Calot in 1891 as bounded by the cystic duct, the right hepatic duct and lower edge of liver 4. In its present interpretation the upper border is formed by the inferior surface of the liver with the other two boundaries being the cystic duct and the common hepatic duct 2,5. Its contents usually include the right
hepatic artery (RHA), the cystic artery, the cystic lymph node (of Lund), connective tissue and lymphatics 5,6. The cystic artery is a branch of the RHA and is usually given off in Calot’s triangle 7.
Anatomic variations in Calot’s triangle are common.
Variations in cystic artery anatomy, based on its origin,
position and number are well described 3, 8 because of its
importance in avoiding inadvertent bleeding and its
consequences. The reported incidence of these variations is
from 25 to 50 % in various studies 3,9 with the magnified
laparoscopic view having increased the frequency of
recognition of these variations. The methods of retraction
used in the laparoscopic procedure gives a different view of the area, thus introducing the term ‘laparoscopic anatomy’7.
Accurate knowledge of cystic artery anatomy and its
variations can reduce the likelihood of uncontrolled intraoperative bleeding, an important cause of iatrogenic extra hepatic biliary injury and conversion to open
cholecystectomy 3, 7, 8. The incidence of conversion to open surgery due to vascular injury is reported to be 0-1.9% and its mortality 0.02% 3, hence these variations should stay in surgical conscience to prevent procedure related morbidity.
We aim to present the variations in cystic artery seen in
laparoscopic cholecystectomy in our patient population.
4.Ferric reducing antioxidant power of essential oils extracted from Eucalyptus and Curcuma species
Shahwar Durre ; Raza Asam Muhammad ; Bukhari Sana ; Bukhari Gulshan
Asian Pacific Journal of Tropical Biomedicine 2012;(z3):1633-1636
Objective: Eucalyptus and Curcuma species are well reputed for their traditional medicinal uses in south east Asia, therefore, the present study was designed to determine reducing potential of their essential oils. Method: Essential oils of the selected medicinal species Eucalyptussideroxylon, E. teriticornis, E. citriodora, Curcuma longa and C. aromatic were extracted using hydro distillation method, separated with diethyl ether and dried over anhydrous sodium sulphate. Column chromatography of Curcuma aromatica was carried out and six fractions were collected using gradient solvent system of n-hexane-ethyl acetate. Ferric reducing antioxidant power (FRAP) of oils were evaluated using standard protocol and results were expressed in μM equivalent to FeSO4.7H2O. Results: The essential oil of Eucalyptus sideroxylon was found to possess highest reducing potential among the Eucalyptus species. Curcuma longa essential oil showed most significant reducing potential with 138.4±1.1 FRAP equivalents. Conclusions:It was concluded that the all essential oil and the column fractions of C. aromatica possess significant reducing capacity ranged from 95.8±1.0 to 152.4±1.4 μM in a dose dependent manner.
5.Clinical Characteristics and Visual Outcomes of Cat Scratch Disease
Jane SHI ; Muhammad Raza CHEEMA
Korean Journal of Ophthalmology 2025;39(3):205-212
Purpose:
To describe the clinical presentation, ocular complications, treatment, and visual outcomes in a series of 17 patients from New Zealand with neuroretinitis secondary to Bartonella henselae.
Methods:
Retrospective cross-sectional single-center study from 2001 to 2024. Data was extracted from a clinical database of all patients treated for B. henselae over the past 23 years. Statistical analysis was performed using IBM SPSS Statistics ver. 26.0.
Results:
Seventeen patients (19 eyes) were included with 11 (65%) being female. The mean age at presentation was 28.3 ± 12.3 years (range, 13–60 years). The mean presenting visual acuity (Early Treatment Diabetic Retinopathy Study testing) was 50 ± 23 (range, 4–90). The mean final visual acuity was 78 ± 12 (range, 45–90). Eleven patients (65%) had cats or kittens at home, with only one (6%) recounting a history of being scratched. Eight patients (47%) demonstrated the characteristic macular star at presentation, with all eventually developing macular star within a mean of 6 days. Four patients (24%) had macular oedema, three (18%) had vasculitis, two (12%) had uveitis, two (12%) had disc granuloma, and one (6%) had multifocal chorioretinitis. Six patients (35%) were treated with rifampicin and doxycycline, one (6%) with rifampicin and azithromycin, two (12%) with doxycycline only, two (12%) with co-trimoxazole monotherapy, and one patient (6%), who was breast-feeding, was treated with erythromycin. Four patients (24%) did not have receive any treatment.
Conclusions
The characteristic macular star is not always manifest at initial presentation for patients with neuroretinitis secondary to B. henselae, nor is there always a preceding history of cat exposure. Not all patients require treatment to have a good visual outcome, and the immune status of the patient is important.
6.Clinical Characteristics and Visual Outcomes of Cat Scratch Disease
Jane SHI ; Muhammad Raza CHEEMA
Korean Journal of Ophthalmology 2025;39(3):205-212
Purpose:
To describe the clinical presentation, ocular complications, treatment, and visual outcomes in a series of 17 patients from New Zealand with neuroretinitis secondary to Bartonella henselae.
Methods:
Retrospective cross-sectional single-center study from 2001 to 2024. Data was extracted from a clinical database of all patients treated for B. henselae over the past 23 years. Statistical analysis was performed using IBM SPSS Statistics ver. 26.0.
Results:
Seventeen patients (19 eyes) were included with 11 (65%) being female. The mean age at presentation was 28.3 ± 12.3 years (range, 13–60 years). The mean presenting visual acuity (Early Treatment Diabetic Retinopathy Study testing) was 50 ± 23 (range, 4–90). The mean final visual acuity was 78 ± 12 (range, 45–90). Eleven patients (65%) had cats or kittens at home, with only one (6%) recounting a history of being scratched. Eight patients (47%) demonstrated the characteristic macular star at presentation, with all eventually developing macular star within a mean of 6 days. Four patients (24%) had macular oedema, three (18%) had vasculitis, two (12%) had uveitis, two (12%) had disc granuloma, and one (6%) had multifocal chorioretinitis. Six patients (35%) were treated with rifampicin and doxycycline, one (6%) with rifampicin and azithromycin, two (12%) with doxycycline only, two (12%) with co-trimoxazole monotherapy, and one patient (6%), who was breast-feeding, was treated with erythromycin. Four patients (24%) did not have receive any treatment.
Conclusions
The characteristic macular star is not always manifest at initial presentation for patients with neuroretinitis secondary to B. henselae, nor is there always a preceding history of cat exposure. Not all patients require treatment to have a good visual outcome, and the immune status of the patient is important.
7.Clinical Characteristics and Visual Outcomes of Cat Scratch Disease
Jane SHI ; Muhammad Raza CHEEMA
Korean Journal of Ophthalmology 2025;39(3):205-212
Purpose:
To describe the clinical presentation, ocular complications, treatment, and visual outcomes in a series of 17 patients from New Zealand with neuroretinitis secondary to Bartonella henselae.
Methods:
Retrospective cross-sectional single-center study from 2001 to 2024. Data was extracted from a clinical database of all patients treated for B. henselae over the past 23 years. Statistical analysis was performed using IBM SPSS Statistics ver. 26.0.
Results:
Seventeen patients (19 eyes) were included with 11 (65%) being female. The mean age at presentation was 28.3 ± 12.3 years (range, 13–60 years). The mean presenting visual acuity (Early Treatment Diabetic Retinopathy Study testing) was 50 ± 23 (range, 4–90). The mean final visual acuity was 78 ± 12 (range, 45–90). Eleven patients (65%) had cats or kittens at home, with only one (6%) recounting a history of being scratched. Eight patients (47%) demonstrated the characteristic macular star at presentation, with all eventually developing macular star within a mean of 6 days. Four patients (24%) had macular oedema, three (18%) had vasculitis, two (12%) had uveitis, two (12%) had disc granuloma, and one (6%) had multifocal chorioretinitis. Six patients (35%) were treated with rifampicin and doxycycline, one (6%) with rifampicin and azithromycin, two (12%) with doxycycline only, two (12%) with co-trimoxazole monotherapy, and one patient (6%), who was breast-feeding, was treated with erythromycin. Four patients (24%) did not have receive any treatment.
Conclusions
The characteristic macular star is not always manifest at initial presentation for patients with neuroretinitis secondary to B. henselae, nor is there always a preceding history of cat exposure. Not all patients require treatment to have a good visual outcome, and the immune status of the patient is important.
8.Clinical Characteristics and Visual Outcomes of Cat Scratch Disease
Jane SHI ; Muhammad Raza CHEEMA
Korean Journal of Ophthalmology 2025;39(3):205-212
Purpose:
To describe the clinical presentation, ocular complications, treatment, and visual outcomes in a series of 17 patients from New Zealand with neuroretinitis secondary to Bartonella henselae.
Methods:
Retrospective cross-sectional single-center study from 2001 to 2024. Data was extracted from a clinical database of all patients treated for B. henselae over the past 23 years. Statistical analysis was performed using IBM SPSS Statistics ver. 26.0.
Results:
Seventeen patients (19 eyes) were included with 11 (65%) being female. The mean age at presentation was 28.3 ± 12.3 years (range, 13–60 years). The mean presenting visual acuity (Early Treatment Diabetic Retinopathy Study testing) was 50 ± 23 (range, 4–90). The mean final visual acuity was 78 ± 12 (range, 45–90). Eleven patients (65%) had cats or kittens at home, with only one (6%) recounting a history of being scratched. Eight patients (47%) demonstrated the characteristic macular star at presentation, with all eventually developing macular star within a mean of 6 days. Four patients (24%) had macular oedema, three (18%) had vasculitis, two (12%) had uveitis, two (12%) had disc granuloma, and one (6%) had multifocal chorioretinitis. Six patients (35%) were treated with rifampicin and doxycycline, one (6%) with rifampicin and azithromycin, two (12%) with doxycycline only, two (12%) with co-trimoxazole monotherapy, and one patient (6%), who was breast-feeding, was treated with erythromycin. Four patients (24%) did not have receive any treatment.
Conclusions
The characteristic macular star is not always manifest at initial presentation for patients with neuroretinitis secondary to B. henselae, nor is there always a preceding history of cat exposure. Not all patients require treatment to have a good visual outcome, and the immune status of the patient is important.
9.Clinical Characteristics and Visual Outcomes of Cat Scratch Disease
Jane SHI ; Muhammad Raza CHEEMA
Korean Journal of Ophthalmology 2025;39(3):205-212
Purpose:
To describe the clinical presentation, ocular complications, treatment, and visual outcomes in a series of 17 patients from New Zealand with neuroretinitis secondary to Bartonella henselae.
Methods:
Retrospective cross-sectional single-center study from 2001 to 2024. Data was extracted from a clinical database of all patients treated for B. henselae over the past 23 years. Statistical analysis was performed using IBM SPSS Statistics ver. 26.0.
Results:
Seventeen patients (19 eyes) were included with 11 (65%) being female. The mean age at presentation was 28.3 ± 12.3 years (range, 13–60 years). The mean presenting visual acuity (Early Treatment Diabetic Retinopathy Study testing) was 50 ± 23 (range, 4–90). The mean final visual acuity was 78 ± 12 (range, 45–90). Eleven patients (65%) had cats or kittens at home, with only one (6%) recounting a history of being scratched. Eight patients (47%) demonstrated the characteristic macular star at presentation, with all eventually developing macular star within a mean of 6 days. Four patients (24%) had macular oedema, three (18%) had vasculitis, two (12%) had uveitis, two (12%) had disc granuloma, and one (6%) had multifocal chorioretinitis. Six patients (35%) were treated with rifampicin and doxycycline, one (6%) with rifampicin and azithromycin, two (12%) with doxycycline only, two (12%) with co-trimoxazole monotherapy, and one patient (6%), who was breast-feeding, was treated with erythromycin. Four patients (24%) did not have receive any treatment.
Conclusions
The characteristic macular star is not always manifest at initial presentation for patients with neuroretinitis secondary to B. henselae, nor is there always a preceding history of cat exposure. Not all patients require treatment to have a good visual outcome, and the immune status of the patient is important.
10.Prevalence of Ascaridia galli in white leghorn layers and Fayoumi-Rhode Island red crossbred flock at government poultry farm Dina, Punjab, Pakistan
Hafiz Allah Bachaya ; Muhammad Asif Raza ; Muhammad Ashraf Anjum ; Imran Ahmad Khan ; Abdul Aziz ; Zahid Manzoor ; Shaukat Hussain Munawar
Tropical Biomedicine 2015;32(1):11-16
Poultry farming not only provides high nutritious food but also creates employment
opportunity for rural masses. Documented evidences elaborates that helminth parasitism is
most deciduous problem of chickens especially in developing world. Ascaridia (A.) galli, a
nematode of small intestine, has been considered as the most common and important parasite
of chicken. The present study was carried out to investigate prevalence and severity of A.
galli in White Leghorn layers (housing type: battery cage and deep litter, 50 each) and
Fayoumi-Rhode Island Red crossbred (male and female: 50 each) flock rearing at Government
Poultry Farm, Dina, Punjab, Pakistan. Two hundred faecal samples were examined by using
standard parasitological and McMaster egg counting technique. The overall prevalence was
24.5% at farm, 13% in White leghorn layer (battery cage=2%, deep litter=24%) and 36% in
Fayoumi-Rhode Island Red (male=34%, female=38%). It was also observed that White leghorn
layer rearing in deep litter had more severe infection (EPG=1920) of A. galli compare with
battery cages birds (EPG=500). Parasite prevalence was significantly related with sex (P<0.05)
in Fayoumi-Rhode Island Red and male birds had less number of average parasites (0.34±0.47)
as compared to females (0.38±0.490). Additionally, female birds were under serious threat of
infection (EPG=2270) compared with its counterpart (EPG=1250). Given the high infection
rates, particular attention should be paid to management and provision of feed supplement to
White leghorn layer housing in deep litter and female bird of Fayoumi-Rhode Island Red
crossbred.