1.Toxoplasma gondii virulence prediction using hierarchical cluster analysis based on coding sequences (CDS) of sag1, gra7 and rop18
Didik T SUBEKTI ; Fitrine EKAWASTI ; Muhammad Ibrahim DESEM ; Zul AZMI
Journal of Veterinary Science 2021;22(6):e88-
Toxoplasma gondii consists of three genotypes, namely genotype I, II and III. Based on its virulence, T. gondii can be divided into virulent and avirulent strains. This study intends to evaluate an alternative method for predicting T. gondii virulence using hierarchical cluster analysis based on complete coding sequences (CDS) of sag1, gra7 and rop18 genes. Dendrogram was constructed using UPGMA with a Kimura 80 nucleotide distance measurement. The results showed that the prediction errors of T. gondii virulence using sag1, gra7 and rop18 were 7.41%, 6.89% and 9.1%, respectively. Analysis based on CDS of gra7 and rop18 was able to differentiate avirulent strains into genotypes II and III, whereas sag1 failed to differentiate.
2.Early outcome of cardiac surgery in dialysis-dependent end-stage renal failure patients
Kee Soon Chong ; Cheong Ping Pau ; Muhammad Ibrahim Azmi ; Mohamed Ezani Md Taib ; Jeffrey Jeswant Dillon
The Medical Journal of Malaysia 2020;75(1):43-46
Introduction: Preoperative dialysis-dependent renal failure
is a strong independent risk factor for in-hospital mortality
and morbidity after open heart surgery. This retrospective
study analyses the early outcome in dialysis-dependent
renal failure patients who underwent elective open-heart
surgery in the Institut Jantung Negara (IJN).
Methods: We retrospectively analyse a series of 228
consecutive postoperative patients with dialysis-dependent
(end stage renal failure (ESRF)) admitted to the adult
cardiothoracic ICU in IJN between January 2012 and
December 2016.
Results: The overall early mortality rate included 34 patients
(15.8%). Patients with ESRF underwent combined procedure
recorded a very high mortality rate at 56.3%. Twenty-four
patients (11.2%) needed resternotomy for postoperative
bleeding or cardiac temponade. Postoperative mediastinitis
rate was high, involving 13 patients (6%). The neurological
and gastrointestinal complications rate were recorded at
2.3% (5 patients) and 6% (13 patients) respectively. In the
group of patients (n=199) with sinus rhythm during the
preoperative period, 100 patients (50.3%) developed
postoperative AF. 77 patients (35.8%) stayed in hospital for
more than 14 days.
Conclusions: dialysis-dependent patients undergoing
cardiac surgery poses higher perioperative risk of mortality
and morbidity of 3-4 times higher compared to those
patients with normal renal function. IJN shows acceptable
perioperative risk of mortality and morbidity which is
comparable to other centres