Child ; Female ; Forecasting ; Humans ; Mass Screening ; Metabolic Diseases ; diagnosis ; etiology ; Pediatrics ; trends ; Pregnancy ; Prenatal Diagnosis

Neonatal stroke is one of the main causes of neonatal death and neurological sequalae. However,the therapeutic strategies for neonatal stroke have not been proven effective. Research advances of neonatal stroke including its incidence,clinical manifestation,neuroimaging are discussed in this review,and the diagnostic evaluation and managements to minimize the sequelae of neonatal stroke are also discussed.

[Objective] To study the clinical effect of the decoction of warming kidney and notifying spleen added with 1a(OH)D3 on senile osteoporosis.[Method] Administer 180 cases with the decoction and 1a(OH)D3 3 tablets;test the BMD change of lumbar vertebra and left thighbone before and after treatment,record the low back pain as well.[Result] After 3 courses,BMD was increased by 2.02% for low back,it was by 1.82% for thighbone;there’s difference between before and after treatment;82 cases were markedly relieved on low back pain(45.56%),76(42.22%)much relieved;there’s no marked difference on Ca,P and AKP.[Conclusion]The decoction and 1a(OH)D3 can improve bone concentration,increase bone intensity,relieve pain and symptoms.

Purified T lymphocytes of syngenic mice were obtained by nylon-wool filtration followed by EAC rosette sedimentation.Passive intravenous transfusion of immune T lymphocytes to normal syngenic mice showed a mild degree of protection. After being challenged with B. anthracis spores, the survival rate of the recipients of immune T lymphocytes was 77.8%,while that of normal T lymphocytes was 42. 9%.Neonatally thymectomized mice offered less humoral immune response than the sham-thymectomized mice after immunization of adsorbed antigen. The G M T of the former was 1:1.4, and that of the latter was 1:3.0 as determined by counterimmunoelectropho-resis.Reconstitution of T lymphocytes of the thymectomized mice improved the production of specific antibody. This result suggests the helping function of T lymphocytes during the process of formation of antibody.Humoral immunity appears to be the major factor in the protection against B. anthracis infection, as the immune serum offered better protection than immune splenic cells.

Objective:To isolate and screen the active marine microorganisms from East China Sea and to identify the phenotypes of the isolated active strains.Methods: The samples were widely collected from East China Sea and the active strains were screened out through an anti-bacterial model and a cytotoxic model.The isolated strains were subjected to phenotypical study,salt-aggregation test and rDNA ITS sequencing.Neighbor-joining phylogenetic trees were drawn by comparing the results of rDNA ITS sequencing with sequences of described species in the BLAST server of the National Center for Biotechnology Information(NCBI).One of the strains,subsequently named XD20,was identified to species level.Results: XD20,a halophilic strain with a highest tolerable salt concentration of 10.0% and suitable concentration range of 2.5% to 7.5%,was collected and isolated on the site of C01(122?26′30″,30?43′48″) 30 meters under water.XD20 showed an inhibitory effect on the growth of Candida albicans and a cytotoxic activity toward SMMC7721 and HeLa cells.XD20 produced arthroconidia by mycelium break and the arthroconidia had similar size and morphology to those of Wallemia sebi.rDNA ITS sequencing showed that the sequence of XD20 had a higher similarity to those of Wallemia sebi and the differences were located in ITS1 and ITS2 areas,both belong to variable region.Conclusion: XD20,an active halophilic strain fungus,has been obtained from the sea for the first time,and has been identified to be Wallemia sebi(Fr.) Arx.

Objective To obtain stable arsenic-resistance cells,the fetal bone marrow mesenchymal stem cells(BMSCs)were exposed to low-level arsenite for 18 weeks. Methods Cells from 4 months fetal bone marrow were cultured in ?-MEM medium to obtain BMSCs.After 24h cytotoxicity test of the fetal BMSCs,we chosed 1?mol/L NaAsO_2 to be the dose under which the cell death-rate was 5%-10%.The fetal BMSCs were exposed to low-level arsenite.MTT was used to detect the survival rate and IC_(50) of arsenic-exposed cells and the control cells,which can reflect the change of arsenic tolerance.A hydride generationatomic fluorescence spectrometry method was used to detect arsenic in the arsenic-resistance cells and the control cells.In order to study the mechanism of arsenic-resistance,we also examined the intracellular GSH and GST content in the arsenic-resistance cells and the control cells. Results The fetal BMSCs were continuously exposed to 1?mol/L NaAsO_2.Parallel cells were cultured in medium without arsenic provided passage-matched control.After the fetal BMSCs were continuously exposed to low level NaAsO_2 for 18 weeks,cells exhibited dramatic resistance to acute arsenite toxicity.Compared to control cells,arsenic-resistance cells showed reduction in arsenic accumulation in cells.The GSH levels and GST activity of arsenic-resistance cells were higher than that of control cells.Conclusion Acquisition of stable arsenic-resistance in BMSCs was available by using cell culture and adding low-level arsenic.Our research established a solid basis for further study about the mechanisms of arsenic-resistance in human being.

Objective: To establish a two-dimensional polyacrylamide gel electrophoresis(2-DE) technique for serum proteomics.Methods: Various conditions of serum proteomic 2-DE were adjusted and optimized.Results: A steady 2-DE technique was established.Conclusions: Our established 2-DE technique of serum proteins can be effectively applied in serum proteomics of diseases.

0.05).The differences of necrosis rates of tumors in all the three groups(0.36?0.18 in group A,0.11?0.09 in group B and 0.10?0.06 in group C) had statistically shown the same results mentioned above. Conclusion: Intratumor injection of ~(90)Y-SAg is a safe,feasible and effective method to treat liver cancer in mice.

Objective:To establish a method for isolation,purification,and culture of human multipotent adult progenitor cells(MAPCs) in vitro,so as to lay a foundation for the application of hMAPCs in tissue-engineering research and clinical medicine.Methods: The mononuclear cells were obtained from bone marrow of healthy volunteers by gradient centrifugation and were subjected to adherence culture.The adherent cells were then subjected to magnetic activated cell sorting(MACS)(depletion selection with CD45 and GlyA microbeads).Then the purity of selected cells was identified by flow cytometer.The growth of the purified cells was observed and the expression of CD29,CD44,CD34,and HLA-DR was analyzed by flow cytometers.Results: (1)Averagely(5-10)?10~(4)/ml hMAPCs could be separated from 1?10~(6)/ml bone marrow mononuclear cells by MACS.The cell viability was similar before(96.7?1.7%) and after(96.0?2.4%) isolation.(2)The isolated MAPCs grew well in the self-designed culture medium and could be passaged for 20 generation.(3)The purity of the CD45~-and GlyA~-cells separated from bone marrow adherent cells was more than 98% as determined by flow cytometer.(4) In hMAPCs,the positive rate was 99.2% for CD29,98.3% for CD44,1.2% for CD34,and 5.3% for HLA-DR.Conclusion:(1)The bone marrow-derived hMAPCs can be purified by MACS through depletion selection of CD45 and GlyA microbeads.(2)The hMAPCs can be expanded in vitro in self-designed medium,maintaining a non-differentiation state for a long time.

OBJECTIVE To investigate the quasispecies diversity and the dynamics of quasispecies evolution in patients with chronic Hepatitis C virus(HCV) infection.METHODS Changes of HCV quasispecies before and after interferon(IFN) therapy were detected by heteroduplex mobility analysis(HMA) and by automatic sequencer.RESULTS We found that no changes in viral complexity were observed with therapy,but there were marked changes in viral divergence.The proportion of major pretreatment variants was different in HVR1 in all patients.During the treatment,HCV quasispecies changed significantly.CONCLUSIONS HMA is a simple,rapid and reliable method for detecting HCV quasispecies.Responsiveness to interferon may be related to the changes in viral divergence,but not related to the numbers of viral variants.