Aims: This study aims to investigate the phylogroups, antibiotics susceptibility and biofilm formation among CVEC isolated from female with bacterial vaginosis. Methodology and results: High vaginal swab from girl with age (18-60 years) were collected and cultured on MacConkey agar, EMB agar and UTI chromogenic medium to recover CVEC and only the confirmed Escherichia coli will pass through rest of the assays like phylogrouping (by PCR), antibiotics susceptibility test and biofilm formation. The results revealed that only 32 (20.38%) of CVEC were recovered and among them only 3 (9.375%) of CVEC belong to intestinal subgroup A1 and the rest 29 (90.625%) assigned to extraintestinal phylogenetic group B2. CVEC isolates belong to B1 and D groups not reported. Antibiotics resistance results shown that, 32 (100%) for cefazolin, cephalothin, cefoxitin and metronidazole, 31 (96.9%) for erythromycin, 24 (75%) for fosfomycin, 20 (62.5%) for cefotaxime, 16 (50%) for ceftazidime, 14 (43.75%) for cefepeim, (28.1%) for aztreonam, 7 (21.9%) for streptomycin, 6 (18.75%) for meropenem, 5 (15.6%) for both imipenem and gentamicin, 2 (6.25%) for both ciprofloxacin and norfloxacin, amikacin 1 (3.1%) and no resistance stated for nitrofurantion (0.00%). TCP methods results display that 12 (37.5%) of CVEC were biofilm former while 20 (62.5%) were non biofilm former. Conclusion, significance and impact of study: This study concluded that, most of the CVEC belong to highly virulent phylogroup B2 and have the ability to resist multiple antibiotics and the ciprofloxacin, norfloxacin, amikacin and nitrofurantion still the best choice for treatment and CVEC have the ability to form biofilm which make the infection ruthless and hard to cure.
Vaginosis, Bacterial