1.The effect of regulator proteins on the IFN-γ/TLR9 synergistic signal transduction
Baljinnyam T ; Khulan O ; Erkhembayar Sh ; Baasansuren E ; Jawkhlan B ; Batkhishig ; Enkhsaikhan L ; Galindew B ; Tsewelmaa N ; Baigalmaa B ; Hongorzul B ; Sodnomtsogt L ; Nyambayar D ; Batbaatar G ; Monhbat B ; Munkhtuwshin N ; Bilegtsaikhan Ts
Health Laboratory 2018;8(1):8-13
Introduction:
When human body encounters external pathogens primary/innate immunity cells are activated by recognizing them and secondary/adaptive immunity is activated consecutively. Immune cell surface receptors, called Toll-like receptors (TLRs) recognize and bind pathogens. In our previous study, we revealed that there is a synergistic action between TLR9 and IFN-γ signaling in the endothelial cells.
Purpose:
To determine the role of negative and positive regulatory proteins on the IFN-γ/TLR9 synergistic signaling pathway
Materials and Methods:
This study was held in the Core Laboratory, Science Technology Center, Mongolian National University of Medical Sciences (MNUMS). In this study, murine endothelial cell (END-D) culture was used. The negative and positive regulator protein expression was detected by Western blotting.
Result:
Result of immunoblotting assay indicated that CpG DNA enhanced IFN-γ positive regulator protein p38 phosphorylation in the endothelial cells. Treatment by TLR9 ligand CpG DNA and IFN-γ increased p38
activation in 0.5 hour and 1 hour. CpG DNA inhibited IFN-γ negative regulator SOCS1 protein expression in 4 hr and 8 hr. Therefore, TLR9 ligand CpG DNA increased IFN-γ signal transduction in the endothelial cell line.
Conclusion
TLR9 ligand CpG DNA has decreased IFN-γ negative regulator protein SOCS1 expression. CpG DNA has increased IFN-γ positive regulator protein p38 phosphorylation.
Result Analysis
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