Introduction: The use of ionizing radiation in diagnostic radiography might lead to hazards such as genetic work practices on radiation protection could minimize these risks. Thus. Therefore, we conducted this study to assess the knowledge and use of radiation safety precautions among healthcare workers (HCWs) of the Diagnostic Radiology Department who are exposed to ionizing radiation in the workplace. Methods: This is a cross-sectional study of 93 HCWs from Egypt. Data collection was through a quasi-self-administered questionnaire and performance checklist. Safety measures in different units of the Diagnostic Radiology Department were assessed using a workplace observation checklist. Results: More than 90% of the study participants had good knowledge about radiation hazards, and all HCWs reported good knowledge about exposure dose and monitoring. Furthermore, 87% of participants had good knowledge of personal protective equipment (PPE), and 74.2% adequately used PPE during work. All doors and walls of Diagnostic Radiology Department units were composed of lead material, and a radiation safety officer was available. Conclusion: The majority of HCWs were aware of occupational health and safety measures and had good knowledge about radiation hazards. All Diagnostic Radiology Departments need to continue professional development by providing more workshops, training courses, preparation, and posters on the protection and safety toward ionizing radiation regarding the most recent regulations to improve the knowledge and performance of their staff.