Aims: This study is focused to explore Pseudomonas aeruginosastrain mass transport andassessment of the water quality.Methodology and results:Pseudomonas aeruginosahave been detected in the real location at the RBF study areabyusing molecular method. The bacterium strain was cultivated in the river water sample and column study was performed using soil aquifer collected from the RBF study site.Conclusions, significance and impact of the study:The column experiments show the removal of the bacteria was achieved almost 99%. Concentration of cultured bacteria was detected in range 0.26 g/Lto 0.33 g/Land reduced after flow through the soilpassage in range 0 to 0.148 g/L. This study also examines the water qualities that affected during the transport such as turbidity and color. The range of turbidity for initial concentration for both influents was 84-96 NTU was reduced to range 2.88-49.29 NTU. Meanwhile for color, the initial concentration was 51-58 PtCo was reduced to range 1-18 PtCo