Introduction: cAMP Response Element Binding (CREB) and Brain Derived Neurotrophic Factor (BDNF) protein levels tend to be a critical mediator for the beneficial effects of diet on neurogenesis and cognitive function. Increased fruit intake has been associated with improved cognitive function. However, in Malaysia, most adolescents still haven't met the recommended intake of fruits and far less is known on how their adequacy of fruit intake can be affected to their protein levels of CREB and BDNF. Methods: A cross sectional study was done on 352 students aged 14 and 16 years old involving 11 secondary schools in Kuala Terengganu, Marang and Hulu Terengganu Districts. A validated MyUM adolescent FFQ was used for fruit intake assessment. Blood sample to determine the level of CREB and BDNF proteins. Results: A total of 53.7% of adolescents consumed an adequate intake (≥ 2 servings/day) of fruits. Serum level BDNF was significantly (p<0.001) higher in the adolescent with adequate fruit intake (389.05±204.96 pg/mL) compared with inadequate (< 2 servings/day) group (118.60 ± 49.04 pg/mL) and was correlated positively with fruit intake (r=0.62, p<0.001). The median of CREB protein level was significantly (p<0.021) higher among inadequate intake of fruits (19.85±23.60 ng/mL) relative to adequate group (14.71± 17.9 ng/mL) and was negatively correlated with fruit intake (rs (8) =-0.07, p<0.001). Apple, papaya, watermelon, orange, pear, guava, mango, banana and durian were significant factors for BDNF protein level. Conclusion: High serum protein level of BDNF in the adolescent with adequate intake of fruit could have a role in controlling neuronal survival, and synaptic function in the central nervous system.

Introduction: This study was carried out to determine the prevalence of vegetable intake and its associated personal, socio-environmental and physical-environmental factors among Malay adolescents in rural Terengganu. Methods: This study was a cross-sectional study conducted from 1st January 2019 to 31st May 2019 at selected secondary schools in rural Terengganu in Marang and Hulu Terengganu districts. A total of 240 students were recruited by multistage random sampling method in schools and based on inclusion criteria. A validated questionnaire was developed consisting of four (4) sections; demographic data, personal, socio-environmental and physical-environmental factors of vegetable intake. Reliability test found the validity and internal consistency of the questionnaire to be acceptable with a Cronbach Alpha value of 0.762. Results: The respondents consisted of 53% males and 47% females with mean age of 15.01 (1.00) years old. Majority of the respondents had normal BMI status (48%) follows by underweight (20%), overweight (16%) and obese (16%). Majority (83%) had inadequate vegetable intake per day. Factors that were associated with the vegetable intake among adolescents were liking (p=0.01), intention (p=0.01) and habit (p<0.01) for personal factor. An availability of vegetable at leisure place (p=0.01) was significant for physical-environmental factor. Conclusion: Majority of the adolescents did not meet the recommended intake of vegetable daily. Therefore, development of intervention programs to promote intake of vegetables among adolescents should consider their liking, habits, intention and the availability at leisure place compare to others in schools or any organizations.

Introduction: The objective of this study was to examine the levels of knowledge, attitudes, and practices of adolescent’s vegetable intake in rural Terengganu, Malaysia. Methods: This study is a cross-sectional study conducted at selected secondary schools in the districts of Marang and Hulu Terengganu from January 2019 until May 2019. By using multistage random sampling in schools and based on inclusion criteria, a total of 160 students was recruited. A self-administered questionnaire consisting of demographic information, knowledge, attitude and practice of vegetable intake was developed by the researchers. The reliability test showed that the validity and internal consistency of the questionnaire were acceptable with a Cronbach Alpha value of 0.701, 0.702 and 0.708 for knowledge, attitudes and practices, respectively. Results: The findings showed that respondents had ‘good’ knowledge (41%), ‘good’ attitudes (41%) and ‘fair’ practices (52%) towards the vegetable intake. There were a significant difference in the mean knowledge scores between male and female respondents (p=0.041) based on the Independent T-test. A positive correlation was observed between attitude and knowledge (r=0.38), attitude and practices (r=0.25) (p<0.05). The result also showed that respondent’s intake of vegetables was significantly correlated with knowledge (p=0.014) and attitudes (p=0.006). Conclusion: The findings showed that only 9% adolescents achieved adequate intake of vegetables per day based on recommendation by Malaysian Dietary Guideline. Therefore, if these population did not change their food behavioural intake, the incidence of chronic diseases was expected to rise during their adulthood.

Introduction: Aquilaria malaccensis, also known as “Pokok Karas” in Malaysia, is widely used in Southeast Asian countries for the treatment of joint pain, diarrhoea and inflammatory diseases, and has shown beneficial effects as an anticancer agent. The aim of this study was to investigate the effect of ethanol leaf extracts of A. malaccensis on MCF-7 cells. Methods: MTT-based cytotoxic and antiproliferative assay was used to determine the outcome of ethanolic extract toward MCF-7 cells. The mode of cell death was determined by the AO/PI double staining assay and the depolarisation of the mitochondria membrane potential. Results: IC50 value of the extract against MCF-7 cells treated for 72 hours was 4.1 ± 2.08 µg/mL, while the IC50 value for doxorubicin was 2.92 ± 0.12 µg/mL. The extract showed a lower cytotoxic effect against the NIH/3T3 cells and inhibited the growth of MCF-7 cells in a dose dependent manner. AO/PI double stain showed that the ethanolic extract of A. malaccensis leaves induced MCF-7 cells into apoptotic cell death. The present study showed that the ethanolic extract of A. malaccensis induced apoptosis through mitochondrial pathway as indicated by its ability to take up JC-1. Conclusion: The study found that ethanolic extract obtained from A. malaccensis leaves is cytotoxic on MCF-7 cells, resulting to apoptotic cell death of the cells.