Dengue Fever is a major public health issue in Malaysia. This study aimed to assess knowledge, attitude and practice regarding dengue fever and effectiveness of health education programme among 204 students of Alam Shah Science School, Cheras/Kuala Lumpur. Study design was a cross sectional study followed by an interventional (pre-post) Study. The self-administered questionnaire included questions on sociodemographic factors, knowledge, attitude and practice regarding dengue fever. Majority of respondents were Malays (96.6%), monthly family income more than RM 3000 (64.6%) and lives in urban area (64.6%). There was no significant association between knowledge status and socio-demographic factors (p-value>0.05). Most of the respondents had good knowledge (63.2%) good attitudes (79.9%) regarding dengue fever. However, practices to prevent dengue was poor (74.0%). Only about one-fifth of the respondents (18.2%) believed that dengue fever is a flu-like illness. Knowledge score was significantly increased after health education programme (p value <0.001). There is a need to increase health education activities through campaigns and mass media to increase knowledge regarding dengue fever. This would help to inculcate positive attitudes and cultivate better preventive practices among the public to eliminate dengue fever in the country.