Aims: Mobile phones are used extensively by healthcare workers (HCWs) who are unaware of the amount of contamination these phones carry and act as reservoirs for organisms causing hospital-acquired infections. This investigation was aimed to find the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) contamination and antimicrobial resistance patterns of isolates from HCW’s cellphones. Methodology and results: This study was a hospital-based cross-sectional study. A total of 120 samples were isolated from HCWs’ cellphones and subjected to culture and sensitivity as per the standard guidelines. Five (18.1%) out of 120 collected samples were from 11 lab technicians’ phones and were positive for S. aureus and 6.25% were obtained from 48 nurses’ cellphone swabs. Conclusion, significance and impact of study The findings of this study reveal that HCWs’ cellphones could be apossible source of infection since a high prevalence of MRSA was found on lab technicians and nurse cellphone sample swabs. On the other hand, all S. aureus isolates were resistant to methicillin and ceftazidime. Also, no significant relationship between the prevalence of MRSA was detected.