OBJECTIVE:To enhance pharmaceutical enterprises' awareness on social responsibility and facilitate their understanding of their social responsibility.METHODS:The motivations for pharmaceutical enterprises to bear social responsibility and their social responsibility at different levels were analyzed by combining theory with practice.RESULTS & CONCLUSIONS:The motivations for pharmaceutical enterprises to bear social responsibility arise from both interior and exterior factors.In order to facilitate the understanding of pharmaceutical enterprises at different stages of development about their social responsibility and achieve a long-term development by realistically assuming their responsibility meanwhile promoting social welfare,it is advisable to divided their social responsibility into 3 levels:basic level,medial level and high level.

Objective To observe effect of home nursing on prevention and control of asthma. Methods Emergency patients(85 cases) were divided into the treatment group(42 cases) and the control group (43 cases).The control group received routine medical treatment after emergency management.The treatment group was given family intervention based upon routine medical treatment,the family intervention included regular visits or telephone followup,short message or internet multimedia, etc.These interventions supplied correct medication method,self-monitoring of asthma, self first-aid at acute attack,how to seek medical emergency treatment and psychological support,ete.The followup continued for 1 year. The asthma control test (ACT) was adopted to compare the clinical effect of the two groups. Results The control rate of the treatment group was greatly improved compared with that of the control group (P < 0.05). Conclu-sion Family intervention played a pivotal role in the improvement of control rate of asthma.

OBJECTIVE: A im To investigate the role of qercetin(Que) -induced autophagy in the death of hepatocellular carcinoma cell line SMMC-7721 cells. METHODS: After treatment with different concentration of Que, the growth inhibition of the SMMC-7721 cells were assessed by MTT colorimetric assay. The fluorescent staining was applied to identify the autophagy after Que treatment. Lactate dehydrogenase (LDH) leakage and Western blot analysis were used to study the autophagic mechanisms involved in death of SMMC-7721 cells. RESULTS: The proliferation of SMMC-7721 cells were significantly inhibited in a dose and time-dependent manner after Que treatment. Autophagy was also induced in MCF-7 cells as detected by MDC staining and Fluorescent staining in the early phase. The autophagy specific inhibitor 3-MA or chloroquine potentiated Que's cytotoxicitiy in SMMC-7721 cells when administered 1h before Que; The expression of cathepsin B increased after Que treatment. CONCLUSION: Que can significantly inhibit the growth of the SMMC-7721 cells by inducing the autophagy, which is a protection mechanism that can reduce the cytotoxicity induced by Que in SMMC-7721 cells. The activation of Cathepsin B is considerable in autophagy process.

Objective To establish a new method for the identification of Cortex Phellodendri. Methods A HPLC fingerprint at 220 nm has been established for identification of Cortex Phellodendri, using a Merck-Lichrospher RP-C_(18) column(4.6?250mm, 5?m) and ACN(A) with the buffer of 0.3% H_3PO_4-NH(CH_2CH_3)_2 (B) in gradient as the mobile phase. The results were compared with the chromatograms of three species of Rbizoma Coptidis under the same conditions. Results HPLC fingerprint of Cortex Phellodendri at 220nm consists of 14 specific peaks. The steady appearance of the peaks and their relative contents were considered as important signs for the identification of this crude drug. The chromatograms of Rhizoma Coptidis were obviously different from the fingerprint of Cortex Phellodendri. Conclusion This method has been proven to be feasible for the identification of Cortex Phellodendri.

Objective To establish a method for determination of Berberine and Phellodendrine in Cortex Phellodendri(CP).Methods Reverse-phase HPLC method was used for determination of Berberine and Phellodendrine in different batches of CP.The chromatographic conditions were:Merck-lichrospher RP-C18 column(4.6?250 mm,5 ?m),flow rate being 0.8 mL/min,column temperature at 25 ℃,detection wavelength at 284nm for Phellodendrine and 245nm for Berberine.Results The content of Berberine was 4 times and that of Phellodendrine in Chuan CP 2～3 times as much as that of Guan CP.Conclusion The method is proved to be feasible for quality assessment of Cortex Phellodendri.Limited contents of Berberine and Phellodendrine should be laid out for the great difference in Chuan CP and Guan CP.

Atherosclerosis is a chronic inflammatory vascular disease developing from early fatty streaks to highly rupture-prone unstable plaques.Many cellular and molecular events are involved in each step.With the development of targeting probe technology,molecular magnetic resonance imaging techniques are expected to revolutionize the treatment strategies of atherosclerosis in the near future.Many desirable molecular probes targeting various components of plaque have emerged in recent years.This article reviews the molecular magnetic resonance imaging techniques of atherosclerosis and their application.

Objective To explore the clinical nursing methods for the treatment of the skin degloving injury in foot by the combination of free femoral anterolateral skin flap transplantation and vacuum sealing drainage (VSD) technique. Methods From January 2010 to January 2014, 18 cases of the skin degloving injury in foot were treated by debridement and implant skin, negative pressure drainage and irrigation at first period, then the operation to repair the foot wound by transplant femoral anterolateral skin flap were done at second period. Nurses applied the key technology of VSD into postoperative nursing. Nurses should master the observation method and characteristics for the flow of blood to the flap skin, the method for foot position nursing, afford effective analgesic care, and flap local high flow oxygen therapy,improve the local tissue oxygen partial pressure and introduce the foot early functional exercise during postoperative. Results Eighteen cases flaps all survived. Clients were followed-up for 6 to 12 months,the discovery showed that all of the clients had good flap blood supply,the skin was soft and elastic,and the function of foot were recovered well. Conclusions The combination of of free femoral anterolateral skin flap transplantation and VSD technique apply into foot degloving injury repairation postoperative care,by application of high quality nursing, it really can improve the success rate of surgery and reduce the pain for clients.

Objective: To study the expression of F1 ATPase-α in non-small cell lung cancer and its clinical significance. Methods: expression level of F1 ATPase-α was examined by immunohistochemical Envision method in 38 NSCLC samples and the adjacent normal tissues, 7 benign tumors and chronic pneumonia samples. (2) F1 ATPase-α mRNA expression were detected in 12 fresh samples of NSCLC and the adjacent normal tissues by PT-PCR. (3) The expression of F1 ATPase-α on the pericellular membrane of A549 cells was observed by immunofluorescence. Results: (1) Immunohistochemistry analysis showed median and higher expression of F1 ATPase-α in 36 of NSCLC specimens and 11 adjacent normal tissues; lower expression was detected in all the benign samples. The overexpression of F1ATPase-α in lung adenocarcinoma tissues were significantly higher than that in the lung squamous cancer (P<0.05). The expression of F1 ATPase-α in NSCLC was not associated with the histology type, location, differentiation degree, size, invasion and metastasis. (2) The relative level of ECTO-F1ATPase-α was 0.31±0.12 in the adjacent normal tissues and 0.54±0.19 in NSCLC tissues (P<0.01). (3)The conspicuous positive expression of F1 ATPase-α on the pericellular membrane of A549 cells was not observed on normal bronchial epithetial cells. Conclusion: (1) NSCLC has a higher expression of F1 ATPase-α and the expression is on the pericellular membrane of A549 cells, which may provide a new target for molecular therapy of NSCLC.

Objective To explore the pathological characteristics, diagnosis and operative treatment of Mirizzi Syndrome(MS). Methods The clinical data of 95 cases of Mirizzi Syndrome admitted to our hospital in recent 12 years were reviewed retrospectively. Results All of the 95 cases underwent operative therapy. In the 95 patients, simple cholecystectomy was performed in 2 patients, cholecystectomy and choledochotomy with T tube drainage in 58 patients, cholecystectomy and choledoscopic bile duct examination via cystic duct in 15 patients, partial cholecystectomy plus repair of choledochal fistula in 18 patients, and cholecystectomy plus repair of the injured choledochus in 2 patients. 65 cases were folloned up for 1-5 years, and all of them have been in good health. Conclusions Mirizzi Syndrome consists of varied pathology. The selection of rational operation for different types of Mirizzi Syndrome can give satisfactory results.