Aim To investigate the antagonistic action o f total saponins of panaxnotoginseng(PNS) on cardiac hypertrophy and its nervous mechanism.Methods (1)cardiac hypertrophy of rats due to pressure overload was induced by constricting of abdominal aorta. There were five groups in the experiments. The rats in Group A(control group)were sham operated . Group B was aorta-constricted group.The rats in Group C,D,E were given ip PNS 50,100,150 mg?kg?d -1 for three weeks respectively. Three weeks later, We measured the heart-weight(HW),left ventricular weight(LVW), the ratio of HW/BW,LVW/BW (LVI) and the cardiomyocyte diameters(MD) after dyeing by HE color.(2)The effects of PNS on the fast excitatory postsynaptic potential(f-EPSP),membrane depolarization induced by application of acetylcholine (ACh),membrane potential and membrane resistance of the isolated Stellate ganglion(SG)of the rats were investigated by means of intracellular recording techniques. Results (1)HW/BW, LVI and MD of Group E were significantly lower than that of Group B(P0.05).(2)At the concentration of 0.10 ～0.16 g?L -1, PNS reversibly depressed the amplitude of f-EPSP, but the ACh depolarization,membrane potential and membrane resistance were not significantly altered by PNS. Conclusion PNS can prevent cardiac hypertrophy due to pressure overload in rats and this action may underline its inhibitory action on presyn aptic effect of regulating calcium influx.

Objective To establish a method for the determination of Paracetamol in Zihua Ganmao Capsule. Method Samples were separated on a Kromasil-C18 column with the mobile phase of methanol- water (35∶65), and the detection wavelength was set at 249 nm. Result The calibration curves were linear in the range of 0.165～0.99 ?g (r=0.999 9) for Paracetamol. The average recovery was 98.41% and RSD= 0.64% (n =6). Conclusion The method is accurate and repeatable, and can be used for the quality control of Zihua Ganmao Capsule.

Aim To study the effect of rhynchophylline on NMDA receptor 2B subunit(NR2B) mRNA expression in the nucleus accumbens(NAc) and amygdala(Amy) of amphetamine-induced condictioned place preference(CPP) rats.Method 56 male SPF grade Sprague-Dawley rats were randomly divided into control group,amphetamine-induced model group,model group with ketamine,model group with low,middle,high dose of rhynchophylline,and control group with rhynchophylline(n=8 for each group) The time of rats' staying in white compartment was measured in the 5th day,then NR2B mRNA expression in NAc and Amy of each rat was detected with in situ hybridization technology.Results The rats exhibited CPP after treated with 2 mg?kg-1?d-1 amphetamine for 4 d.Ketamine and rhynchophylline eliminated CPP effect,and the effect of rhychophylline showed a dose-dependence.In amphetamine-induced model group,NR2B mRNA expression in NAc and AMY of rats was significantly increased.Ketamine,middle and high dose of rhynchophylline blocked the increase of NR2B mRNA expression induced by amphetamine.Rhynchophylline had no effect on the basal level of RN2B mRNA expression in normal rats.Conclusion Inhibition of NR2B mRNA expression in NAc and AMY may contribute to the antagonistic effect of rhychophylline on amphetamine-induced CPP.

The development of DNA chip has been strongly driven by modern approaches of life science and microelectronics. The broad of DNA chip applications include the detection of pathogens,the detection of mutations in genes involved in human diseases or affected during cancer progression, sequence analysis, the pattern of gene expression monitoring and pharmacy studies .This review explains the technology, its scope, and impact on medicine, as well as its cost and possible limitations.

Objective To understand the implementation status and effect of prevention and control of malaria in Libo Coun?ty so as to provide the evidence for improving the malaria elimination working. Methods The data about malaria from the county CDC and county hospital were collected and 16 villages from 8 townships were randomly sampled and 10 villagers of each village were investigated. Other information about the prevention and control of malaria was also investigated. Results The inci?dence of malaria was decreasing annually from 5.75 per 10 000 in 2008 to zero in 2012. The malaria monitoring could be well conducted in the county and township levels. The infection source could be controlled in time. The utilization rate of anti?mosqui?to facilities in the residents was 93.25%and the awareness rate of knowledge about malaria prevention and control was 40.13%. Conclusions The implementation and effect of prevention and control of malaria are satisfactory in Libo County but the medi?um control is limited and the active protection consciousness of the residents is not strong. Therefore the task of malaria elimina?tion is still very arduous.

Objective To introduce the clinical application of the improved interosseous dorsal antidromic artery issular flap in the emergency treatment of the soft tissue defects in hand trauma. Methods 16 patients were received the interosseous dorsal antidromic artery flap for soft tissue defects in the hand with emergency treatment. Results All 14 flaps survived, tip necrosis occurred in 2 cases which healed without complications. Conclusion It was simple technical and shorten time in hospital with improvement of the emergency operative procedure of interosseous dorsal antidromic artery flap, and could reduce the impact of frequency of anatomic variations and vascular complications.

AIM: A determination was created of sinomenine and tanshinnone Ⅱ A in Shuanghuoqufengzhentong Wine (Caulis Sinomenii, Radix Salviae Miltiorrhizae, etc.) at the same time by HPLC. METHODS: The method contained a Waters ? PORASIL TM P/N27477 column with methanol water ethylene diamine (90∶10∶0.125) as mobile phase, UV detection wavelength was set up at 262nm. RESULTS: The average recovery of sinomenine was 97.9%, the RSD was 2.83%. The average recovery of Tanshinnone Ⅱ A was 99.0%, The RSD was 1.96%. CONCLUSION: The method is rapid, accurate, sensitive. This study provides a testing method for the quality control of similar products.

The human ?3-adrenoceptor plays an important role in the regulation of energy mobilization and utilization. A missense mutation in codon 64 of the gene for the ?3-adrenoceptor with a replacement of tryptophan to arginine (Trp64Arg) was found. This polymorphism influences receptor function when expressed in artificial cell lines or in isolated human fat cells. It is associated with an earlier onset of type 2 diabetes mellitus, features of insulin resistance and tendency to gain weight. Recent evidences suggest that Trp64Arg has additive and interactive effects with a number of other candidate gene variants. The functional significance of human ?3-adrenoceptor polymorphism and its association with metabolic diseases are reviewed in this paper.

Objective To determine the expression of E-cadherin-catenins complex(E-cad,?-cat,?-cat and ?-cat) in early gastric cancer and their relationships with biological characteristics of early gastric cancer by evaluating the invasion and the lymph nodes metastasis of eraly gastric cancer(ECG).Methods 53 cases of EGC,who accepted radical operations from January 1997 to December 2000 in Sir Run Show Hospital were included in this study.According to the invasion depth and lymph nodes metastasis,they were divided into different sub-groups for discussion.Also the normal gastric mucosa of 10 cases with extra-gastric disease were selected as control group.Results All four proteins(E-cad,?-cat,?-cat and ?-cat) were normally expressed in control group.In early gastric cancer,the abnormal expression rates of E-cad,?-cat,?-cat and ?-cat were 64.2%,69.8%,66% and 73.6%,respectively.In the sub-group of mucosal invasion,the abnormal expression rates were 61%(14/23),65.2%(15/23),47.8%(11/23) and 56.5%(13/23),respectively.But in the sub-group of submucosal invasion,the abnormal expression rates were 66.7%(20/30),73.3(22/30)%,80%(24/30) and 86.7%(26/30),respectively.In sub-group of no lymph node metastasis,the abnormal expression rates were 61.4%(27/44),65.9%(29/44),63.6%(28/44) and 70%(31/44) respectively.In sub-group of lymph node metastasis,the abnormal expression rates were 77.8%(7/9),88.9%(8/9),77.8%(7/9) and 88.9%(8/9) respectively.Abnormal expression of ?-cat and ?-cat had significantly difference between the sub-group of mucosal invasion and the submucosal invasion(P