Objective Metrological literature analysis of breast reconstruction from 1987 to 2008 in China,in order to illuminate the process of breast reconstruction's development and to predicts the mian tendency of breast reconstruction in the future.Methods Papers about breast reconstruction from 1987 to 2008 on Chinese Medical Current Content (CMCC) and Chinese Bio-medical Literature Database (CBMdisc) were Metrologically analyzed according to journal,institute,surgeon method,optimal time,content and so on.Results In all 247 collected,the first place in order of first jourual,institute,author,area,surgeon methods,optimal time were as follow:Chinese Journal of Aesthetic and Plastic Surgery (15 papers),China Chinese Academy of Medical Sciences and Peking Union Medical College (46 papers),Qi Fazhi (9 papers),Beijin(56 papers),latissimus dorsi perforator flap breast reconstruction,and transverse rectus abdom in is myocutaneous flap breast reconstruction,immediate reconstruction.Conclusions This paper which outlines some features of papers published in China on breast reconstruction in rencent 21 years provides a credible resource for further study.The immediate latissimus dorsi perforator flap breast reconstruction would be the main trend of it's further development.

Objective To explore the efficacy of somatostatin and pantoprazole sodium in treatment of severe acute pancreatitis. Methods 124 cases with severe acute pancreatitis admitted in the People's Hospital of Zhoushan City from Jan 2011 to Jan 2014 were randomly divided into control group and combination group,each had 62 cases. Patients in control group were treated with pantoprazole sodium,while in combination group were treated with somatostatin and semi Pantoprazole sodium. Results The total effective rate in combination group was 95.16%,higher than 64.52% in control group. the difference was significant(P<0.05 ). The recovery time and the incidence of complication in combination group was significantly lower than control group (P <0.05 ). Conclusion Somatostatin and pantoprazole sodium can effectively improve the treatment efficiency of SAP patients,shorten recovery time,and reduce the incidence of complication.

Total glucosides of paeony (TGP, 50 mg/kg 7d, ig) could enhance the episode duration of slow-wave sleep (SWS) in normal rats, and restore the sleep parameters in the insomniac rats induced by caffeine ( 1 2. 5 mg/kg 7 d,ip)nearly to the normal level. It (50 mg/kg 3 d,ig) also increased significantly thetotal time of SWS and paradoxical sleep (PS) in the swimming rats (water temperature 25?1℃, swimming time 30 min). These results suggest that TGP probably improve the sleep of rats under the different states.

Objective To summarize the literature of autologous stem cell transplantation (ASCT) for the treatment of diabetic foot from 2004 to 2009 in China through analyzing its development, the main operative methods as well as the future development tendency. Methods Papers about breast reconstruction from 1987 to 2008 on Chinese Medical Current Content (CMCC) and Chinese Bio-medical Literature Database (CBMdisc) were metrologically analyzed according to journal, institute, operative method, time, content and so on. Results In all 105 papers collected, the first place in terms of journal, institute, author, area, operative method was as follows:Chinese Journal of Clinical Rehabilitation (6 papers), Beijing Capital Medical University (10 papers), Gu Yongquan (9 papers), Beijin (17 papers). Autologous bone marrow stem cell transplantation (ABMSCT) and autologous peripheral blood stem cell transplantation (APBSCT) are the main operative methods. Conclusions This paper provides a credible resource for further study through analyzing papers published in China on stem cell transplantation for treatment of diabetic foot in recent years. ABMSCT and APBSCT must be the main trends in the future. Before formulating strict indications, ABMSCT and APBSCT will be the main operation modes of ASCT. Quantifying indications of ASCT and coupling with other drugs in order to make better role of ASCT will be the main subject of the study.

To established an effective GC-MS /MS method for the contents determination of the residual DEHP in injection equipment, and investigate the effect of the pretreatment on the measurement. To simulate the clinical conditions of use, under the condition of 37 degrees C balance extraction, extract liquor by chloroform extraction, then the extract followed by analysis of GC-MS /MS. The method was simple, rapid, sensitive and accurate. The limits of quantitation (LOQ, S/N = 5) of cyclohexanone was 0.075 μg/mL, The spiked average recoveries ranged from 92% to 98%. The relative standard deviations (RSDs) of the method ranged from 1.01% to 1.61%, The method was simple, fast, sensitive and accurate, and may serve as a mass control method for residual DEHP in injection equipment.
Cyclohexanones ; chemistry ; Diethylhexyl Phthalate ; chemistry ; Equipment Contamination ; Gas Chromatography-Mass Spectrometry ; Injections ; instrumentation ; Limit of Detection ; Plasticizers ; chemistry

AIM To study the effect of astragalosides (AST) on pr ol iferation and collagen production of hepatic stellate cells (HSC). METHO DS The proliferation and collagen production of rat hepatic stellate cell s, HSC-T6, stimulated with Kupffer cell-conditioned medium (KCCM), mixed sera containing new bovine serum (NBS) and rat serum (RS) were measured with 3H -TdR and 3H-proline incorporation. RESULTS Both of the p roliferation and collagen production of HSC-T6 stimulated with KCCM at the dilu tion of 1∶4 were suppressed after being treated with AST (16, 32, 64, 128 and 2 56 mg?L -1 ). When HSC-T6 cells were stimulated with mixed sera containing 10% NBS and 3% RS, their proliferation was suppressed after being treated with AST(32, 64, 128 and 256 mg?L -1 )for 48 h and so was the collagen produ ction after being treated with AST(16, 32, 64, 128 and 256 mg?L -1 )for 72 h. CONCLUSION The suppression of hepatic stellate cell prolif eration and collagen production by astragalosides may be one of the mechanisms f or the depression of hepatic fibrosis by astragalosides.

Objective To observe the effect of Xijiaodihuang decoction on the release of inflammatory mediators and prognosis in elderly patients with sepsis.Methods A prospective randomized controlled study was conducted. Seventy-four patients with sepsis admitted to the Department of Geriatrics of Changshu Hospital of Traditional Chinese Medicine (TCM) Affiliated to Nanjing University of TCM from March 2015 to February 2017, and they were divided into a TCM treatment group and a control group randomly, 37 cases in each group, there were 2 patients transferred to other department during the period of study to continue treatment, 2 patients discharged automatically and 1 case transferred to other hospital in the TCM treatment group; and there were 3 patients transferred to other hospital, 1 patient discharged automatically, 1 patient dead in 7 days after entering the control group and 1 patient transferred to other department during the period of observation to continue treatment in the control group. Finally, 32 patients were in TCM treatment group and 31 patients in the control group, completing the study. All patients in the two groups received conventional treatment of sepsis, patients in the TCM treatment group took Xijiaodihuang decoction 100 mL concentrated [rhinoceros horn (replaced with buffalo horn) 30 g, rehmannia root 24 g, peony 12 g, tree peony bark 9 g] taken orally or by nasal feeding, and patients in the control group received the same amount of normal saline, the two groups were treated 1 time a day for consecutive 7 days to complete 1 therapeutic course, then the treatment efficacy was evaluated in the two groups. The differences of serum interleukins (IL-1β, IL-6), tumor necrosis factor-α (TNF-α), white blood cells (WBC), C-reaction protein (CRP), lactate levels between the two groups were compared before treatment and on the 3rd, 7th and 14th day after treatment, the incidence of staying in intensive care unit (ICU), time of staying in ICU and 28-day mortality were also observed. The 28-day survival rate between two groups was analyzed by Kaplan-Meier survival curve.Results After treatment in the two groups, the levels of IL-1β, IL-6 and TNF-α had a tendency of increase at first and then decrease. After treatment for 14 days, the IL-1β was significantly lower in the TCM treatment group than that of the control group (ng/L: 83.27±21.84 vs. 96.73±26.33), the levels of IL-6 and TNF-α in TCM treatment group were obviously lower than those in the control group since 7 days after treatment [IL-6 (ng/L): 48.27±24.13 vs. 62.15±24.34, TNF-α (μg/L): 1.41±0.31 vs. 1.96±0.29]. IL-6 and TNF-α were still lower than those in the control group until 14 days after treatment [IL-6 (ng/L): 29.25±18.57 vs. 56.24±23.61, TNF-α (μg/L) 1.35±0.28 vs. 1.83±0.22, allP < 0.05]. There was no significant difference in WBC and CRP between the two groups before and after treatment (bothP > 0.05). After treatment, the lactate in the control group was gradually decreased, while in the TCM treatment group, the lactate level after treatment presented a tendency firstly decreased and then elevated, and after treatment for 3 days in TCM treatment group, the level of lactate began markedly lower than that in the control group (mmol/L: 1.26±0.43 vs. 2.01±0.59,P < 0.05). The ICU hospitalization rate and ICU length of stay in the TCM treatment group were significantly lower than those in the control group [56.25% (18/32) vs. 83.87% (26/31), (10.2±5.4) days vs. (13.5±5.8) days], and the 28-day mortality was also obviously lower in TCM treatment group than that in control group [9.37% (3/32) vs. 29.03% (9/31)]. Kaplan-Meier survival curve analysis showed that the 28-day survival rate in TCM treatment group was significantly higher than that in control group (P = 0.045).Conclusion Xijiaodihuang decoction can reduce the levels of IL-1β, IL-6, TNF-α and lactate in serum, reduce the incidence of staying in ICU, decrease the time of staying in ICU and improve the prognosis of elderly patients with sepsis.

Analyzed the purity of the Perfluorooctane used for Ophthalmic operation using the FID (Flame Ionization Detector) method after the qualitative analysis of that. Research and discussion of the 1--hydrogen perfluorocarbon octane, which is the typical impurity of Perfluorooctane. The standard recovery of the 1--hydrogen perfluorocarbon octane is 93%-97% within the range of linearity, and the RSD (relative standard deviation) is below 2%. The above test method has the advantages of easy operation, stability and accuracy. So it is a good reference support to bring the specification of quality control of the Perfluorooctane which is used for Ophthalmic operation into YY of medical device.
Fluorocarbons ; analysis ; chemistry ; Ophthalmology ; instrumentation ; methods ; Quality Control

Cerebral ischemia/reperfusion injury （CIRI） is a common feature and the main pathophysiological mechanism of ischemic stroke（IS）， which is caused by a blood reperfusion injury in ischemic brain tissues. It can aggravate brain tissue injury and cause irreversible brain damage， seriously affecting the quality of life or even the life of patients. Hence， we must find out the exact mechanism as well as the effective therapeutic drugs and targets for CIRI. The Chinese medicine effective in Xingnao （restoring consciousness） and Kaiqiao （opening orifices） has been widely used in the treatment of CIRI and serves as a classic therapy for IS. In recent years， scholars have conducted extensive and in-depth studies on the mechanism and therapeutic targets of Chinese medicine in Xingnao and Kaiqiao. They found that those drugs could interfere with a series of changes after IS and achieve the remarkable curative effect. This study summarized the effect and mechanism of Chinese medicine in Xingnao and Kaiqiao in the treatment of CIRI， including reducing the inflammatory response and oxidative stress， alleviating brain edema and the toxicity of excitatory amino acids， reducing cell apoptosis， promoting angiogenesis and neurovascular remodeling， and improving blood-brain barrier injury. It is expected to provide references to clarify the mechanism and important targets of those drugs in resisting CIRI and ideas for the in-depth investigation and application of brain protection of Chinese medicine in Xingnao and Kaiqiao.

Due to the reform of medical system, sudden infectious diseases and the increasing demand of patients, the problems of insufficient allocation of human resources and low quality of nursing human resources in China not only affect the treatment and prognosis of patients, but also hinder the development of nursing itself. In order to improve the current situation of nursing staffing in China, this paper summarizes the RAFAELA system of nursing resource allocation in Finland and the need for improvement. In order to promote the improvement and development of the optimal allocation of nursing human resources in China.