1.Analysis of Nucleosides in Different Parts of Cordyceps Sinensis
Zhengming QIAN ; Miaoxia ZHOU ; Mintian SUN ; Zhu LIU ; Xiaojing ZHANG ; Wenqing LI ; Guangrong LI ; Wenjia LI
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(11):2390-2394
A HPLC method for simultaneous determination of uridine, inosine, guanosine, adenosine and cordycepine
in Cordyceps sinensis was developed. The sample solution was prepared with 0.5% phosphoric acid solution by ultrasonic extraction. The separation was performed on a ZORBAX SB-AQ (150 mmí4.6 mm, 5 μm)column with gradient elution by 0.1% formic acid solution and acetonitrile, with column temperature 30℃, at a flow rate of 0.8 mL/min, and detected at wavelength of 260 nm. The result of method validation showed that the developed method had high accuracy and good repeatability. This method has been successfully applied for analysis of 5 kinds of nucleosides in different parts of C. sinensis. The results indicated that content of nucleosides in stroma is higher than that in insect body and whole C. sinensis.
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