Epithelial cellular adhesion molecule (EpCAM)is a transmembrane glycoprotein expressed in epithelial tissues and most epitheli-al-derived malignant tumors.EpCAMis associated with cell adhesion,migration,proliferation,and differentiation,as well as tumor devel-opment and progression.In recent years,EpCAMhas been identified as a stem cell marker of hepatocellular carcinoma (HCC).This paper elucidates the roles of EpCAMin the diagnosis,treatment,and prognosis of HCC,which shed light on the potential molecular targeted thera-py in future.

Objective To construct human EpCAM eukaryotic recombinant plasmid ,and to study the expression of EpCAM pro‐tein in HepG2 cells .Methods The recombinant plasmid ,named pcDNA3 .1(+ )‐EpCAMwas constructed by cloning EpCAM gene into eukaryotic vector pcDNA3 .1(+ ) .Then HepG2 cells were transfected with EpCAMrecombinant plasmid .The eukaryotic ex‐pression of EpCAM protein was verified by immunofluorescence ,Western Blotting and flow cytometry .Results The construction of EpCAM recombinant eukaryotic plasmid was identified by restriction enzyme digestion .The results of immunofluorescence , Western Blotting ,and flow cytometry consistently indicated that EpCAM protein were successfully expressed in HepG2 cells .Con‐clusion Human EpCAM eukaryotic vector is constructed and EpCAM protein could be expressed well in HepG2 cells ,which laid a foundation for further research on the function ofhepatocarcinoma cells highly expressing EpCAM .

ObjectiveTo explore the inductive action of docosapentenoic acid(DPA) on neurite outgrowth in PC12 cells in vitro.MethodsNeurite outgrowth in PC12 cells was examined after the treatment with different concentration of DPA using Motic Zamges Plus software mapping cell image system.Western blot was performed to detect the expression of β Ⅲ-tubulin regulated protein kinase,a neuronal marker as well as ERK and protein kinase B (Akt) phosphorylation.ResultsPC12 cell neurite formation rate was increased in a concentration dependent manner in the induction of DPA,increased by 2.4% (DPA 10 μg/ml,P>0.05),18.6% (DPA 30 μg/ml,P<0.05) and 25.0% (DPA 50 μg/ml,P<0.05) compared with that in the control group.DPA promoted the expression of β Ⅲ-tubulin (P<0.05) and the phosphorylation level of ERK and Akt (P<0.05,P<0.01).ConclusionDPA promotes PC12 cell neurites growth and its mechanism may be related to the activation of ERK and Akt signaling pathways.

Objective To study the application effect of comprehensive nursing intervention in elderly intestinal polyps enucleation. Methods A total of 78 elderly patients with intestinal polyps enucleation were randomly divided into con-trol group(n=39) and observation group (n=39), two groups were given routine nursing,observation group was given com-prehensive nursing intervention based on routine nursing. Results SAS and SDS scores of the observation group after nursing intervention were (37.43±5.63)scores and (48.82±7.46)scores, those were lower than (52.16±8.37)scores and (52.16±8.31)scores of the control group (P<0.05). Intestinal polyps knowledge awareness and nursing satisfaction of ob-servation group were 94.87%and 97.44%, higher than 79.49% and 82.05% of the control group (P<0.05). Conclusion Comprehensive nursing intervention in elderly patients with intestinal polyps enucleation during perioperative can effec-tively relieve patients' anxiety and depression, and improve the recognition of intestinal polyps and nursing quality.

Objective: To establish the quality standard for Shenzhi Xiaoping capsules. Methods: The characteristics of Astragali Radix were identified by microscopy. TLC was adopted to identify Astragali Radix, Fructus Aurantii and Glycyrrhiza uralensis. The con-tent of ginsenoside Rb1in Panax ginseng was determined by HPLC. Results: The microscopic characteristics were obvious. The TLC spots were clear with highly specific identification and good separation. The linear range of ginsenoside Rb1was 47. 06-376. 5 μg· ml-1(r=0. 999 9), and the average recovery was 102. 7% with the RSD of 1. 1% (n=6). Conclusion: The method is simple and specific, which can be used for the quality control of Shenzhi Xiaoping capsules.

Objective To investigate the psychological resilience status and its influencing factors of lung cancer patients. Methods A total of 182 patients with lung cancer were selected by convenient sampling method and were investigated the psychological resilience with Chinese Version Connor and Davidson′s resilience scale ( CD-RISC ) , the Chinese version of medical coping modes questionnaire ( MCMQ ) , and the hospital anxiety and depression Scale ( HAD) . Results The total score of resilience in 182 lung cancer patients acquired (65. 2 ± 15. 0). The total score of resilience in 82 preoperative patients was (68. 9 ± 15. 4), postoperative patients (63.5 ±13.6), chemotherapy patients (60.3 ±14.3); resilience status showed down trend (F=5.530,P<0. 05). Different educational background, marriage status, medical insurance type, treatment stage and cancer metastasis had difference of psychological resilience (P<0. 05). The average anxiety score of 182 cancer patients was (5. 72 ± 4. 52) and depression score obtained (4. 74 ± 4. 39). The correlation analysis presented that lung cancer patients′psychological resilience had negative correlation with anxiety, depression and surrender while it had positive correlation with confrontation (P <0. 05), but had no correlation with avoidance (P>0.05). Multiple regression analysis revealed treatment stages, copying strategies and depression were the main influencing factors which all explained 45. 8% of total variation. Conclusions Resilience of patients with lung cancer are at a lower level, which effected by many factors. Nursing staffs should pay attention to patients psychological status, take effective measures to help them to get higher level of psychological resilience, so as to improve quality of life.

Objective To understand the incidence and death patterns of viral hepatitis in China and provide evidence for the prevention and control of viral hepatitis.Methods The analysis was conducted on the incidence and death data of viral hepatitis published by National Health and Family Planning Commission during 2004-2013.Results The incidences of viral hepatitis in Guizhou,Yunnan,Tibet,Gansu,Qinghai,Ningxia and Xinjiang provinces (autonomous region) were high.The major forms were hepatitis B (80.63/100 000) and hepatitis C (9.68/100 000),accounting for 80.90％ and 9.25％ of the total reported viral hepatitis cases respectively.The incidences of hepatitis A and unidentified hepatitis decreased and the incidence of hepatitis B,C and E increased during this period.During the 10 years,10 008 deaths caused by viral hepatitis were reported (1 001 deaths per year).The reported deaths caused by hepatitis A,hepatitis E and unidentified hepatitis decreased during this period.The reported deaths caused by hepatitis B were in a downward trend,but the constituent in total cases remained high.The reported deaths caused by hepatitis C were in an upward trend.Conclusion During 2004-2013,the overall incidence of viral hepatitis showed no downward trend in China.The incidence of hepatitis B remained high,and the incidence of hepatitis C showed an obvious upward trend.The overall death rate and case fatality rate of viral hepatitis showed a downward trend,but hepatitis B remained the main cause of viral hepatitis related death,and the death caused by hepatitis C was in increase.Hepatitis B and hepatitis C are the major targets in the prevention and treatment of viral hepatitis in China,and the 7 western provinces (autonomous region) with high incidences are the key regions of the prevention and control.

Objective: To establish the quality standard for Fuzheng capsules. Methods: TLC was adopted to identify Astragali Radix and Glycyrrhiza uralensis. The method validation for Fuzheng capsules was conducted by microbial limit test as described in the appendixes of Chinese Pharmacopeia (2015 edition). The content of epimedii in Epimedium brevicornu was determined by HPLC. The chromatographic separation was carried out on an Agilent TC-C18(2) (250 mm×4. 6 mm, 5 μm) column. The mobile phase consis-ted of acetonitrile-water( 30: 70) with gradient elution at a flow rate of 1. 0 ml·min-1,and the injection volume was 5 μl. The detec-tion wavelength was 270 nm. Results: The spots in TLC were clear without any interference. The methods of plating and direct inocu-lation could be used for the microbial limit test. The linear range was 0.101-1.008 μg (r =0.999 7). The average recovery was 99. 36% with the RSD of 0. 81% (n=6). Conclusion: The method is simple with high specificity and good repeatability, which can be used as the quality control method for Fuzheng capsules.

OBJECTIVE:To analyze the rationality of proton pump inhibitors (PPIs) in the prevention of stress ulcer in perioperative orthopedic patients of our hospitals,and to provide reference for ratioual use of PPIs in clinic.METHODS:The medical records of discharged orthopedic patients were selected during Jan.1st-30th in 2016.Those records were analyzed statistically in respects of the use of PPIs,general information of patients,operation situation,the stressors and risk factors of stress ulcer.The rationality of the use of PPIs was analyzed comprehensively.RESULTS:Among 664 surgical patients,PPIs were used in 210 cases,and H2 receptor antagonist (H2RA) were used in 74 cases,with prophylactic rate of 42.77％.The main types of operation were fracture fixation (74 cases,35.24％),discectomy and discectomy (38 cases,18.11％).Among 210 patients receiving PPIs,all of them used PPIs,among which 68 cases (32.38％) had no medication indications,128 cases (60.95％) were medication timing error,74 cases (35.24％) were administration frequency error and 44 cases (20.95％) were more than 3 d prophylactic drug use.CONCLUSION:When orthopedic patients use PPIs for the prevention of stress ulcer,there are serious irrational phenomena in indications,species selection,usage and dosage,treatment course and so on.Clinicians and regulators should pay great attention to it so as to avoid the excessive application of PPIs.