Phospholipase A2(PLA2)is a class of enzymes with the ability to catalyze hydrolysis of lipoproteins and glycerophospholipid in the membrane, mainly including secreted PLA2 ( sPLA2 ) , cytosolic PLA2 ( cPLA2 ) , Ca2+-independent PLA2 ( iPLA2 ) and lipoprotein-associated phospholipase A2 ( Lp-PLA2 ) .They are closely related to many diseases.The studies of the role of PLA2 in cardiovascular diseases have been a hot topic in recent years.This paper focuses on the recent advances in research on the effects of PLA2 on cardiovascular diseases in animal experiments.

Background Hypertension is a multigenetic inheritable disease.Gene therapy by regulating gene expression showed long term effects and less side effects,and has been emerged to be a potential and prospective treatment.Objective To investigate the effects of retroviraladeno-associated virus vector containing shRNA targeted on the AT1R gene (rAAV-AT1R-shRNA) on blood preesure(BP) in renal hypertensive rats,and the inhibitory effect of RNA interference (RNAi) on AT1R mRNA expression in renal hypertensive rats.Methods Two-kideny one-clip (2K1C) renal hypertension (RH) were established in SD rats and randomly to receive rAAV-AT1R-shRNA,1.5?109 particles/mL(n=12,ip),as treated group or retroviral vector (rAAV-EGFP),2.9?109 particles/mL,ip,as vehicle group,normal SD rats served as controls (n=12).SBP was measured before and after treatment.Animals were euthanized and blood,brain,heart,liver,kidney,aorta and adrenal gland were collected to identify the sites of rAAV-AT1R-shRNA expression by fluorescence microscope.Angiotensin Ⅱwas assessed by radioimmunology.Results 24 hours after single injection of rAAV-AT1R-shRNA,SBP was reduced by (22.3?5.5)mmHg compared to before intervention (P

Anthrax is a fatal infectious disease of human and livestock and is caused by Bacillus anthracis. To establish a simple and specific assay for the clinical diagnosis of B. Anthracis infection, two oligonucleotide primers specific for the cap region of plasmid pXO2 and two specific for the pag region of plasmid pXO1 were designed and synthesised for polymerase chain reaction (PCR). The fluorescence dye SYBR Green I incorporated into the double strands was used to quantitate, and the specificity of PCR product was confirmed by the melting curve. The results showed that 0.8?mol/L primers and 3.0mmol/L Mg 2+ were optimal for this quantitative PCR assay. The sensitivity of this assay was 10 3 copies per millitier B. anthracis could be specifically distinguished from other B. cereus group of bacteria. with this assay. SYBR Green I real-time PCR appeared to be a rapid, accurate and specific methad for quantitative analysis of B. anthracis.

Objective:To evaluate the interventional effects of Yufeining on lung function in patients with chronic obstructive pulmonary disease (COPD). Methods:38 patients with COPD were divided into therapeutic group (20 cases) and control group (18 cases) randomly. Therapeutic group was treated with oral Yufeining pill half a year and then was followed up 6 months without drug. Control group was not given any medicine during the observed one year. Routine lung function was measured at before 、6 months and 12 months after treatment. Total symptom score (TSS) of traditional Chinese medicine、the scores of quality of life (QOL) were recorded simultaneously. Blood gas analysis was measured only at before and 6 months after. Results:FEV 1 was 1 032?0 336L after 6 months treatment in therapeutic group, while FEV 1 was 0.982?0.353L before treatment (P

[Objective]To determine the effect of Oxfenmino hydrochloric acid on delayed allergic reaction in mouse. [ Method ] 120 ICR mice weighing 18~22g, half female and half male, were randomly and averagely divided into 6 groups according to weight:control group and model group (treated with distilled water at a dosage of 10ml/kg), three Oxfenmino hydrochloric acid groups (treated with Oxfenmino hydrochloric acid at a dosage of 10.0mg/kg, 5.0mg/kg and 2.5mg/kg respectively) and positive drug group (treated with CsA at a dosage of 15mg/kg); they were orally administered one time every day. After a continuous week of administration, all the groups were smeared evenly with 1%DNFB solution on the abdomen for allergy except the control group. Five days after allergy, 1%DNFB solution was smeared to right ear of all mice to stimulate the allergic reaction except that the control group was smeared with non-allergenic substance. 24 hours after attack, the auricle swelling, spleen index and thymus index in right mouse were determined. [ Result] Different dosages of Oxfenmino hydrochloric acid benefited to reduce auricle swelling, spleen index and thymus index in mouse with allergic reaction induced by DNFB at different degree. [ Conclusion] Oxfenmino hydrochloric acid can inhibit the delayed allergic reaction in mouse induced by DNFB and depress the ability of specific cell-mediated immunity.

AIM: To study the effect of propolis on the expression of CD54 and activation of NF-?B p65 in lung tissue of acute lung injury (ALI) rats. METHODS: 40 male Wistar rats were divided into 5 groups: normal control, model control, dectancyl group, water soluble derivative of propolis (WSP) group and ethanol extracted propolis (EEP) group. ALI animal model was performed by oleic acid and LPS twice attack. The pathologic slice was observed with light microscope and the NF-?B p65 activity and CD54 expression were tested by immunohistochemistry (SABC and SP). RESULTS: Both EEP and WSP antagonized the lung edema, decreased the inflammation and inhibited the expression of CD54 and activation of NF-?B p65. CONCLUSION: The increase in the expression of CD54 and the activation of NF-?B p65 in the lung tissues of ALI were involved in the formation of ALI. Propolis ameliorated the lung damage, which maybe related to the inhibition of CD54 expression and NF-?B p65 activation.

Objective: To investigate the anti-inflammatory effect and the possible mechanism of water and ethanol extracts of propolis. Method: Forty male Wistar rats were divided into 5 groups: normal group, model group, medicine groups, two groups treated with water and ethanol extracts of propolis. The acute pleurisy model was established by injecting carrageenan. The effects of propolis on acute pleurisy was studied by counting leukocytes, measuring the content of MDA, lysozyme and activity of SOD in serum and the content of NO, protein and PGE2 in pleural effusion. Results: The propolis solutions extracted by water and ethanol presented obvious effect on inflammation. It could antagonize the purulent pleurisy, reduce the number of leukocytes and the content of MDA, lysozyme and activity of SOD in serum and the contents of NO, protein and PGE2 and decrease the inflammation. Conclusion: Propolis displays anti-inflammatory effects by decreasing the action of NO and PGE2 and preventing the activation of protein kinase.

Objective To establish a rapid specific quantitative assay for Bacillus anthracis detection. Methods According to the principle of complex probe quantitative assay, the primers and quantitative probes targeted at chromatosome DNA rpoB were designed and applied to detect Bacillus anthracis. The influence factor of quantitative PCR were determined. Results The optimal system of this method was aquired: the length of quenching probe is 15mer,the ratio of fluorescent probe to quenching probe is 1/2 and the concentrtion of Mg 2+ is 3 mmol/L.The sensitivity of this assay for Bacillus anthracis is 10 3 copies. It can distinguish Bacillus anthracis from other closely related Bacillus. Conclusion The method can rapidly quantitatively detect the Bacillus anthracis with high sensitivity and specificity, it can be applied to clinical diagnosis.

Objective To investigate the value and clinical significance of MRI in diagnosis of subcortical trabecular injury in knee.Methods45 patients with obvious pain but no fracture diagnosed by X-ray after knee trauma were scanned with MRI to analyze whether having injuries in subcortical trabecular and knee joint accessory structures.ResultsAll of the 45 cases had normal radiographic results in X-ray examination, but subcortical trabecular injury was found by MRI. MRI demonstrated irregular low signal in the subcortical region on both T1WI and T2WI. The high signal in fat suppressed T22subcortical trabecular and knee joint accessory structures.