ObjectTo investigate the chemical constituents in the rhizoma of Belamcanda chinensis (L.) DC. MethodsThe chemical constituents were isolated and purified by solvent extraction together with various chromatographic techniques. The structures were elucidated on the basis of chemical evidence and spectral data. ResultsThree compounds were isolated from the EtoAC extracts of the rhizome of B. chinensis which were isorhamnetin (Ⅹ), hispidulin (Ⅺ), dichotomitin ( ⅩⅡ); four compounds were isolated from n-BuOH extracts, which were iridin ( ⅩⅢ), tectoridin (ⅩⅣ), daucosterol ( ⅩⅤ), vittadinoside or stigmasterol-3-O-glucoside ( ⅩⅥ). ConclusionCompound Ⅺ is isolated from this medicinal plant for the first time.

Objective To study rDNA ITS sequence similarities and differences between the de-virus seedlings and outside seedlings of Pseudostellaria heterophylla. Methods The sequences of ITS region (including ITS1, ITS2, and 5.8S) from nine de-virus seedlings and four outside seedlings were studied by the method of DNA sequence analysis. Results There were the same sequences in 5.8S region between the de-virus seedlings and outside seedlings of P. heterophylla. Variable sites lay in the initial position of ITS1 and termination of ITS2 basically. The de-virus seedlings from Liyang (Jiangsu Province) and Xuancheng (Anhui Province) had more variable sites. Phylogenetic dendrogram based on ITS was constructed by using UPGMA methods, which showed the inherent relation at the level of molecular biology. Conclusion The analysis of rDNA ITS of P. heterophylla has further clarified the inherit stability of de-virus seedlings at the molecular level.

AIM　The aim is to investigate the chemical constituents of Belamcanda chinensis. METHODS　The chemical constituents were isolated and purified by solvent extraction together with various chromatographic techniques. The stuctures was elucidated on the basis of chemical evidence and spectral data. RESULTS　Eight compounds were isolated from the ethanol extract of the rhizome of Belamcanda chinensis (L.)DC..Among them,six isoflavones were elucidated as irilone(Ⅰ)、genistein(Ⅱ)、tectorigenin(Ⅲ)、 irigenin(Ⅳ)、dimethyltectorigenin(Ⅴ)、irisflorentin(Ⅵ). CONCULSION　 ⅠandⅡ were isolated from this medicinal plant for the first time.

To study the chemical constituents of the fruits of Polygonum orientale L. , silica gel and ODS column chromatography methods were used to separate and purify the constituents. Their structures were elucidated on the basis of physicochemical properties and spectral analysis. Three compounds were identified as 28-O-β-D-glucopyranosyl-3β,7β-dihydroxy-lup-20 (29) -en-28-oate ( 1 ), 5,7-dihydroxychromone (2) and naringenin (3). Compound 1 is a new triterpenoid saponin and others were isolated from the fruits of this plant for the first time.

Object To define the molecular characters of differentiating Bupleurum chinense DC., B. scorzonerifolium Willd. from their allied species B. marginatum Wall. ex DC., B. smithii Wolff var. parvifolium Shan et Y.Li. and B. longiradiatum Turoz. Methods General DNA was isolated from fresh leaves of Bupleurum L. species by CTAB. The random amplified polymorphic DNA (RAPD) was used to identify them. Results Five Bupleurum L. species can be identified by primer OPA-1(5'-CAGGCCCTTC-3'), OPD-8 (5'-GTGTGCCCCA-3'), OPD-11 (5'-AGCGCCATTG-3'). Conclusion The method of RAPD can be used to identify the Bupleurum L. species and its allied species.

Aim: To study new chemical constituents of the leaves of Ginkgo biloba L. Methods: Isolation and purification were carried out by several chromatographic methods. The structures of the compounds were elucidated by detailed analysis of their UV, IR, MS, ~1H NMR and ~(13)C NMR spectra Results: A new ginkgolide, ginkgolide N( 1, 7, 10-trihydroxy-3,14- dehydroginkgolide, Ⅱ), along with a known compound, was isolated from the leaves of G. biloba. The structure of the known one was elucidated as ginkgolide L(Ⅰ). Conclusion: Compound Ⅱ was a new compound. The complete spectroscopic data of compound Ⅰ were reported for the first time.

Objective To evaluate Cica-Beta Test kit for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa (PAE)in the clinical microbiology laboratory.Methods A total of 82 imipenem-resistant PAE clinical isolates from litera-ture[5]was dectected to metallo-β-lactamase (MBLs)by PAE-MHT and Cica-Beta Test kit.Results The sensitivity,speci-ficity and accuracy rate of PAE-MHT was 84.6%,97.2% and 97.6%,and the sensitivity,specificity and accuracy rate of Cica-Beta Test kit was 76.9%,100% and 96.3%,respectively.Two methods had a good consistency.Conclusion Two methods are simple,quick for detecting to metallo-β-lactamase-producing Pseudomonas in clinical laboratories.

To evaluate the differences of Ophiopogonjaponicus from different cultivations, the metabolomics based method was conducted to compare the effects of Hangmaidong and Chuanmaidong (Chinese name) on plasma endogenous metabolites of normal rats. Data were collected by ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS), and were analyzed by multivariate statistical method, such as Principal Component Analysis and Orthogonal Signal Correction Partial Least Square Discriminant Analysis. Results revealed that the plasma metabolites profiling of low and middle dose group of Chuanmaidong were similar to the control group, but different from the high dose group obviously. Meanwhile the high, middle and low dose groups of Hangmaidong were different from control group notably, and the difference is dose dependent. Lysophosphatidylcholines, the possible endogenous metabolites which contribute to the classification most significantly, are closely related to cardiovascular system diseases. Compared with the group of Chuanmaidong, Hangmaidong has greater impact on the plasma metabolic profiling of normal rats. Hangmaidong and Chuanmaidong showed significant differences pharmacodynamically.

OBJECTIVETo propose the seed quality standard of Belamcanda chinensis.

METHODThe seed purity, 1000-grain weight, water content, vitality, germination rate of seed of B. chinensis from different producing areas were measured, and seed characteristics were observed. Cluster analysis was used to analyze to data.

RESULTThe first level seed was lustrously dark and thoroughly rounded, seed purity was above 98%, 1000-grain weight was above 28.00 g, water content was lower than 12.8%, vitality was over 90%, germination rate was over 85%. The second level seed was dark and relatively rounded, seed purity was above 92%, 1000-grainweight was above 20.00 g, water content was below 12.8%, vitality was between 70%-90%, germination rate was between 65%-85%, the third level seed was puce, seed purity was above 86%, 1000-grain weight was above 20.00 g, water content was below 12.80%, vitality was over 50%, germination rate was above 40%.

CONCLUSIONThe seed purity of B. chinensis was almost above 90%, and 1000-grain weight was between 15.27 and 30.76 g. The vitality and the germination rate of B. chinensis seeds from different sources varied obviously.

Cluster Analysis ; Germination ; Iridaceae ; chemistry ; growth & development ; Medicine, Chinese Traditional ; standards ; Quality Control ; Seeds ; chemistry ; growth & development ; Water ; analysis