1.Orthogonal experimental research on the impacts of mandibular plane angle to the stress distribution of dental implants
Weibin XING ; Shuxian ZHENG ; Shiyi YANG ; Minhong HOU
Chinese Journal of Tissue Engineering Research 2017;21(14):2192-2197
BACKGROUND: In clinical practice, the selection of dental implants with different specifications is based on the patient' s mandibular plane angle, and the long-term success rate is different in dental implants with different specifications. Therefore, it is reasonable to assume that the mandibular plane angle could affect the mandibular stress distribution of dental implants, thus affecting the success rate of dental implantation.OBJECTIVE: To perform an orthogonal experimental study on the mechanical effects of different implant specifications, mandibular plane angles and mandibular bone densities on stress distribution of the first mandibular molar implants.METHODS: A L9(33) orthogonal experiment was designed with 3 three-level factors, including mandibular plane angle (14°, 22°, 34°), implant diameter (6.6, 8.2, 9.6 mm), and mandibular bone density (types II, III, IV). Different dental implants with different combinations were implanted into an alveolar bone model, and placed onto a pressure testing machine under 500 N load. Then, strain (stress) data were collected in real-time and analyzed.RESULTS AND CONCLUSION: The optimal combination was as follows: low angle; 4.8 mm in diameter; mandibular bone with type II bone density. The relations of all the three factors were the diameter of dental implant > the mandibular plane angle > mandibular bone density. To conclude, the mandibular plane angle has some certain influences on the stability of dental implants. If the biting force is the same, dental implant bears the largest stress under the high angle,subsequently followed by the average angle and the low angle.
2.Flushing methods, temperature and flushing time of sodium hypochlorite affect the clearance effect on the smear layer of root canal
Minhong HOU ; Min CHEN ; Lei LI ; Shasha ZHANG ; Dongyan ZHOU ; Ligeng WU
Chinese Journal of Tissue Engineering Research 2015;(43):6918-6923
BACKGROUND:In root canal preparation, the clearance effect of sodium hypochlorite for root canal irrigation on the smear layer is associated with the contact time and contact area of irrigation fluid, the amount of irrigation fluid, irrigation fluid temperature, irrigation fluid concentration, flushing methods and so on. OBJECTIVE:To investigate the clearance effect of 3% sodium hypochlorite on the smear layer of root canal under different flushing methods, flushing temperature and flushing time. METHODS:Totaly 180 isolated front teeth with single root were colected and root canal preparation was conducted using motorized ProTaper. The root canal was flushed with 3% sodium hypochlorite. According to different flushing methods, these root canals were randomly and evenly divided into root canal active flushing group, passive ultrasound swished group and side opening flushing needle group. According to the flushing temperature and the flushing time after replacing a file, each group was randomly divided into six subgroups: 20℃ 30 seconds group, 20℃ 60 seconds group, 37 ℃ 30 se group. The clearance effect on the smear layer of root canal was observed using scanning electron microscope. RESULTS AND CONCLUSION:The general clearance effect on the smear layer in the root canal active flushing group was better than that in the passive ultrasound swished group and side opening flush acupuncture group (P< 0.05). At the same time, the clearance effect on the smear layer in the 60℃ group was better that that in the 37 ℃ group and 20℃group (P < 0.05). At a certain temperature, the clearance effect on the smear layer in 60 seconds group was better than that in the 30 seconds group (P < 0.05). The clearance effect on the smear layer in the 1/3 root cap zone was better than that in the 1/3 root and 1/3 apical root zone (P < 0.05). These results show that the best clearance effect on the smear layer can be achieved as folows: 3% sodium hypochlorite irrigation at 60℃ for 60 seconds.
3.Effect of berberine on alveolar bone absorption in diabetic rats
International Journal of Biomedical Engineering 2020;43(3):171-176
Objective:To study the effect and mechanism of berberine intervention on alveolar bone absorption in diabetic rats.Methods:Diabetes rat model was established by one-time intraperitoneal injection of streptozotocin (35 mg/kg). Sixty healthy female Sprague-Dawley rats without specific pathogens were divided into normal group, diabetic group and berberine group according to the random number table method, 20 rats for each group. The rat models in the berberine group and the diabetic group were given berberine (200 mg/kg) and an equal volume of sodium chloride injection by gavage, once a day, for 4 consecutive weeks. The rat models in the normal group were not treated. Fasting blood glucose (FBG), fasting insulin level (Fins) , hormone resistance index (HOMA-IR) and insulin sensitivity index (ISI), as well as the serum calcium, phosphorus content, alkaline phosphatase activity and osteocalcin content of the rats were measured. The pathological changes of periodontal tissues were detected by HE staining. The expression of BMP-2/Smad pathway (BMP-2, Smad1, Smad4) was detected by RT-PCR and Western blot.Results:Compared with the diabetes group, the FBG level of rats in the berberine group [(9.88±1.23) mmol/L vs. (21.66±2.41) mmol/L], Fins levels [(11.63±3.63) mIU/L vs. (21.64±5.58) mIU/L] , HOMA-IR (7.58±0.96 vs. 20.85±1.95) were reduced, and ISI (-5.10±0.16 vs. 6.15±0.18), serum calcium levels[(1.855±0.125) mmol/L vs. (1.317±0.104)) mmol/L], serum phosphorus levels [(3.087±0.278) mmol/L vs. (2.526±0.226) mmol/L], serum ALP levels [(130.681±18.963) U/L vs. (99.874±31.968) U/L] and osteocalcin levels [(112.368±9.752) ng/ml vs. (78.645±10.973) ng/ml] were all increased, and the differences were statistically significant (all P<0.05). The results of hematoxylin-eosin staining showed that there was no bone resorption in the alveolar bone of the rats in the normal group, bone resorption was found in the alveolar bone of the rats in the diabetes group, and the bone resorption of the alveolar bone in the berberine group was significantly reduced. RT-PCR and Western Blot results showed that compared with the diabetes group, the BMP-2, Smad1, Smad4 gene mRNA and protein in the alveolar bone tissue of the berberine group were significantly increased (all P<0.05). Conclusions:Berberine intervention can reduce FBG levels in diabetic rats and improve insulin resistance, which may reduce alveolar bone resorption in diabetic rats by up-regulating the BMP-2/Smad signaling pathway.
4.Application of the team-based learning combined with situational simulation model in standardization training of psychiatric nurses
Linping GUO ; Mingru HOU ; Minhong YAO ; Shilan LIANG ; Linke JI
Chinese Journal of Modern Nursing 2017;23(20):2683-2687
Objective To discuss the implementation effects of the team-based learning combined with situational simulation in the standardized training in psychiatric.Methods A total of 70 psychiatric nurses in the standard training period from June to August 2015 were randomly divided into control group and experimental group, 35 respectively. Nurses in the control group received traditional teaching mode, while nurses in the experimental group used the teaching mode of the team-based learning combined with situational simulation. After the training, the theoretical knowledge, the comprehensive ability of emergency response, and the ability of team work of nurse in two groups were evaluated.Results After 6 months of standardized training, the scores of theoretical knowledge (87.13±7.50) and comprehensive ability of emergency response (95.57±4.19) of nurses in the experimental group were significantly higher than those in the control group (t=8.52, 7.81;P<0.01). The scores of different dimensions of team work ability were (6.58±2.11) of gregariousness, (5.16±2.65) of antorhythmicity, (4.73±2.18) of belongingness and (5.84±1.87) of valuable, which were significantly better than those in the control group (t=-3.36, -6.55, -5.89, -4.51;P<0.05).Conclusions The team-based learning combined with situational simulation model not only increases the knowledge level and comprehensive ability of emergency of nurses, but also improves the team work ability, which can enrich the forms and contents in psychiatric standardized training.
5.Application of AIDET communication mode in the therapy of modified electroconvulsive
Mingru? HOU ; Minhong YAO ; Jingxia FEI ; Yan CAI ; Huihong WU
Chinese Journal of Modern Nursing 2015;21(5):565-567,568
Objective To evaluate the effects of AIDET ( Acknowledge, Introduce, Duration, Explanation, Thanks ) communication mode in the modified electroconvulsive therapy. Methods Firstly, according to the procedure of the modified electroconvulsive therapy, we established the standard phrases of AIDET communication mode. Secondly, 100 cases of hospitalized psychiatric patients from August to October 2013 accepted the modified electroconvulsive therapy and were divided into the control group and the observation group, with 50 cases in each. The control group used the traditional lecture-style communication, while the observation group was given the AIDET communication processes and standard terms. The anxiety of two groups before and after the treatment was compared by the Hamilton Anxiety Scale ( HAMA ) , in addition with the treatment adherence and the satisfaction of nursing. Results Comparison of the HAMA scores in the two groups before the treatment was not significantly different (P >0. 05). After using the two different communication modes, the HAMA scores of somatic anxiety, mental anxiety and the total score of the observation group were significantly lower than those in the control group (t=5. 99, 6. 51, 10. 87, 10. 23, 6. 19, 7. 94, respectively;P<0. 05). The treatment adherence and the satisfaction with nursing of the observation group were significantly higher than those in the control group (Z =5. 77,χ2 =7. 16, respectively;P <0. 05). Conclusions The AIDET mode of communication can effectively reduce the patient′s anxiety, and improve the patients′adherence, treatment compliance and satisfaction.
6.Application of team-based learning combined with situational simulation in clinical teaching of psychiatry
Mingru HOU ; Minhong YAO ; Shilan LIANG ; Linping GUO
Chinese Journal of Modern Nursing 2017;23(5):715-719
Objective To investigate the implementing effect of team-based learning combined with situational simulation in clinical teaching of psychiatry.Methods A compendium was selected from "Psychiatric nursing",which was about the prevention and nursing of psychiatric crisis. We designed curriculums and wrote the scripts,and organized professional scenario walkthroughs. Eventually,we made them into videos and audio data. Firstly,60 nursing students were given team-based learning combined with situational simulation based on traditional teaching for 4 weeks. After teaching,the results of the theory and emergency skills examination and teaching satisfaction were compared.Results After 4 weeks,the professional theoretical examination of nursing students was (88.93±8.20) pints,and it was significantly higher than (83.27±5.31) points before teaching;meanwhile the emergency skills examination was (93.48±5.99) points,which was significantly higher than (85.87±7.34) points before training (t=-7.91,-9.12;P<0.01). The score of satisfaction of nursing students with teaching was (97.21±1.75),which was higher than (83.82±8.23) before training (t=10.78,P<0.01).Conclusions The team-based learning combined with situational simulation teaching not only cultivates nursing students' autonomous learning interest,which could improve independent study ability and effectiveness,but also improves their teamwork skills,which could improve clinical teaching quality and satisfaction.