A postulated suppression of T lymphocyte function by clonidine (an adrenogic receptor agonist) was explored by utilizing mitogen induced lymphocyte transformation of mouse spleen cells in vitro. Added at the onset of the culture or 24h later, clonidine (10-(?)-10-8 M ) did not suppress T lymphocyte transformation induced by ConA or PHA. It implies that there is a difference in the sensitivity for clonidine between T and B lymphocyte, as clonidine has been suggested to suppress the SRBC induced primary immune response of mouse spleen cells in a dose dependent manner.

Objectives To study the relationship between hope and quality of life(QOL)in nasopharyngeal carcinoma(NPC) patients undergoing radiotherapy(RT). Methods One hundred and ten NPC patients undergoing radiotherapy participated in the investigation by the Herth Hope Index(HHI)and the Functional Assessment of Cancer Therapy-head and neck scale(FACT-H&N),to look into the correlations between their hope and QOL.Result The scores on hope and NPC for the QOL patients during radiotherapy were(34.80±2.64)and(91.34±13.19),respectively and they were positively correlated(r=0.253,P<0.01). Conclusions The hope of the NPC patients is at the medium level.Their hope is positively correlated with QOL.The higher level the hope is,the higher the QOL.

Objective To evaluate the influence of pressing technique on the malposition of catheter dur- ing the operation of peripherally inserted central catheter (PICC). Methods Totally, 169 patients with lung cancer were randomly divided into treatment group (n = 88), in which the external and internal jugular veins were pressed at the supraclavicular fossa by the assistant when the catheter was navigated in 25 cm, and control group (n = 81), in which the patients were called to turn their angle of mandible to supraclavicular fossa when the cathe- ter was sent in. Results There were only one patient whose catheter was sent in external jugular vein in treatment group and nine patients whose catheters were sent in external and internal jugular vein in control group. The inci- dence of malposition of catheter was significantly different between two groups (1.1% vs. 11. 1% , X2 =7. 3636, P < 0. 01). Conclusion The pressing technique can reduce the incidence of malposition of catheter into external and internal jugular vein during the operation of PICC.

Objective To investigate the influence of peer education on maintenance knowledge of patients with peripherally inserted central catheter (PICC) outside the hospital.Methods 74 patients with PICC were divided into the intervention group and the control group with 37 patients in each group according to the random digit table,the intervention group received peer education and the conventional health education,while the control group only received routine health education.The maintenance knowledge of PICC was investigated with questionnaires and the results were analyzed.Results xfter intervention,the maintenance knowledge of the intervention group was significantly higher than that of the control group [(36.95±2.84) scores vs.(31.78 ± 4.79) scores,t=5.639,P＜0.05].The incidence rate of complications in the intervention group was lower than that of the control group [21.6％ (8/37) vs.62.2％(23/37),x2=12.491,P＜0.01].Conclusions The peer education can improve patients' maintenance knowledge about PICC and reduce the occurrence rate of the complications of PICC.

0.05).In comparison with pre-treatment,WOMAC scores,pain index,and physiological integral scores in treatment and control groups after the first and the last treatment decreased significantly(P

Mechanism of Immunomodulatory actions of total glucosides of paeony ( TGP ) was studied by McAb indirect immunofluorescence. TGP 5 mg/( kg?d ) ip for 8d suppressed DTH, that was enhanced by cyclophosphamide( Cy), and decreased L3T4+/Lyt-2+ 〔 (Th/i)/(Ts /c) 〕 cell ratio in ICR mice .TGP 5 mg/ ( kg?d ) ip for 5d enhanced DTH that was suppressed by Cy, and increased the L3T4+/Lyt-2 + cell ratio in ICR mice. The same dose of TGP ip for 5d promoted the hemolysin production of spleen cells, that was suppressed by Cy, increased the L3T4+/Lyt-2 + cell ratio in BLAB/c mice. The results suggest that immunomodulatory actions of TGP might be brought about by altering Th/Ts cell ratio.

AIM: To observe the supplementation action of the crude and processed Epimedium Sapittatum Maxim. METHODS: The effects of different extracts of Epimedium Sagittatum Maxim. on the castrated mice were observed. RESULTS: The aqueous and alcohol extracts of the crude and processed Epimedium Sagittatum maxim. showed same marked inhibition on atrophy of castrated mice. CONCLUSION: The crude and processed Epimedium Sagittatum maxim. both have the supplementation action and show no marked difference in action.

Objective To explore the effects of mental practice on upper extremity function after stroke. Methods Thirty sub-acute stroke patients were randomly divided into a treatment group ( n=15 ) and a control group (n=15). The patients in the control group were treated with conventional therapy. The patients in the treat-ment group were treated with motor imagery therapy in addition. All patients were assessed using the Fugl-Meyer mo-tor assessment (FMA) and the motor assessment scale (bIAS) before treatment and after 2, 4 and 8 weeks of treat-ment. Results After 2 weeks of treatment, average MAS scores in the treatment group improved significantly com-pared with before treatment, but there was no significant difference between the two groups. After 4 weeks, FMA and MAS scores in the two groups had improved, and the FMA scores in the treatment group were significantly higher than those of the control group. After 8 weeks, the FMA and MAS scores of both groups had further improved significant-ly, but the average FMA and MAS scores in the treatment group were now significantly higher than those in the control group. Conclusions Mental practice can improve the functional performance of the upper extremities of stroke pa-tients.

Aim To investigate the effect of zacopride ( Zac) on cardiac arrhythmia in isoproterenol ( ISO)-in-duced myocardial hypertrophic rats and the underlying electrophysiological mechanisms .Methods ① Fifty-one rats were randomly divided into control group ( n=17 ) , ISO group ( n=17 ) and ISO +Zac group ( n =17 ) .Rat model with cardiac arrhythmia and hypertro-phy was established by intraperitoneal ISO ( 5 mg?kg -1 ) injection.②ECGs were recorded to observe the effects of Zac on arrhythmia in model rats .③ Whole-cell patch clamp was applied to record inwardly rectifi-er potassium current(IK1), resting membrane potential ( RMP ) and amplicated delayed afterdepolarizations (DADs).Results ① Echocardiographic examination showed that , left ventricular end-diastolic dimension (LVEDD) and left ventricular end-systolic dimension (LVESD) significantly decreased in rats in ISO group compared with control group , whereas left ventricular posterior wall end-diastolic thickness ( LVPWd) and in-terventricular septum end-diastolic thickness ( IVSd ) increased ( P<0.05 ) , suggesting rat model of isoprot-erenol-induced myocardial hypertrophy was successfully established .② ECGs showed that 88.89% of rats in ISO group had ventricular premature beats ( VPBs ) , which significantly decreased to 11.11% after the ap-plication of Zac ( P <0.05 ) .③ Values of RMP de-creased from ( -71.05 ±1.27 ) mV in control group to (-69.38 ±1.21 ) mV in ISO group ( P<0.05 ) . After Zac administration , RMP significantly increased to ( -73.86 ±1.33 ) mV compared with control and ISO group(P<0.05).④DADs and TA incidence sig-nificantly decreased from 88.24% in ISO group to 11.76%in ISO+Zac group ( P<0.05 ) .⑤ Compared with control group , IK1 density was markedly reduced in ISO group, whereas Zac could effectively rescue IK1 suppression to normal level .Conclusions Zac, as a selective IK1 channel agonist , can significantly inhibit cardiac arrhythmia in isoproterenol-induced myocardial hypertrophic rats , which is mainly attributed to in-creased RMP by enhancing IK1 and subsequent suppres-sion of DADs.

Objective To investigate the effect of berberine on the radiosensitivity of cervical cancer cells.Methods 5,10,15,20 μmol/L of berberine were used to treat cervical cancer cell lines of Siha,HeLa,Caski.DMSO was applied as control of drug treatment.Cell proliferation was detected by the CCK-8 method,and then the half inhibitory concentration of berberine was calculated.Cell apoptosis and cell cycle distribution were detected by flow cytometry.Protein expressions of Cleaved Caspase-3,Cyclin B1,CDK1,STAT3 and p-STAT3 were detected by Western blot.Cervical cancer cells of Siha were treated by berberine with a half inhibitory concentration for 24 h and then irradiated with 0,2,4,6,8 Gy of X-rays.Cell clone assay was used to detect cell survival.Results Berberine could inhibit the growth of cervical cancer cells with a half inhibition concentration of(16.84 ± 3.52),(23.54 ± 8.67),(21.86 ± 6.35)μmol/L for Siha,Caski,and HeLa cells,respectively.The berberine at 17 μmol/L could induce apoptosis (t =56.847,P ＜ 0.01) and G2/M phase arrest (t =47.251,P ＜ 0.01) in Siha cells,which also inhibited the expressions of Cyclin B1,CDK1 and p-STAT3 and promoted the expression of cleaved Caspase-3,but did not influence the expression of STAT3 in cervical cancer cells.Treatment of cells with 17 μmol/L berberine increased the radiosensitivity of cervical cancer cells with a sensitivity enhancement ratio of 1.55.Conclusions Berberine can inhibit cell proliferation,promote apoptosis,block cell cycle,and increase radiosensitivity of cervical cancer cells.