This paper analyzes the present situation of health education in coronary artery disease,introduces six kinds of health education models which widely adopted now at home and abroad,such as health education path mode,PRECEDE-PROCEED model,and trans theoretical model.It aims to provide reference for the effective development of health education in coronary artery disease and puts forward prospect of the development of the information construction of health education in coronary artery disease.

Objective To study the effect of curcumin on the expression of inflammatory factors in model rats with chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS) type Ⅲ.Methods The rats were randomly divided into following groups: sham-operated group(sham), CP/CPPS model group(model), curcumin 50 mg group(cur-50 mg) and curcumin 100 mg group(cur-100 mg) and p38 inhibitor group(SB203580).Drug was given to each treatment group for 12 days by intraperitoneal injection.The levels of TNF-α, p38, COX-2 mRNA was measured by Real-Time PCR.The expressions of TNF-α and COX-2 proteins were measured by immunohistochemistry.The levels of p38, p-p38 and NF-κB proteins were detected by Western blot.Results NF-κB, p-p38, TNF-α, COX-2 proteins and TNF-α, p38, COX-2 mRNA in model group were higher than those in the sham group (P<0.01);cur-100 mg group and SB203580 group could significantly relieve these change in the model group (P<0.01);COX-2 mRNA and protein in cur-100 mg group decreased more than that in SB203580 group;p-p38 was positively correlated with NF-κB in curcumin groups and SB203580 group compared with model group (P<0.01).Conclusions Curcumin can reduce the expression of inflammatory cytokines NF-κB, TNF-α, COX-2 and p-p38 in rats with CP/CPPS type Ⅲ.

To strengthen the medical students in patient safety education is the first step to effectively prevent medical accidents.But at present patient safety education has not been given enough attention by domestic mnedical colleges.Research group consulted literature at home and abroad related to patient safety courses, according to the Medical colleges patient safety curriculum guide proposed by The World Health Organization (WHO) Alliance for Patient Safety, opened the patient safety course in undergraduate medical education with the goal to improve the medical students to maintain patient safety consciousness, ability and methods and implemented scene simulation teaching and formative assessment.We achieved good teaching effect and appraisal by students.

Objective To understand the experiences of the disabled ages occurring safety risks in the communities.Methods Phenomenology method was used.13 disabled ages who occurred safety risks in Chongqing participated in semi-structured and in-depth interview,regarding their experiences of occurring safety risks.The data were analyzed using Colaizzi method.Results Four themes were found,including enduring mental stress,lack of safety knowledge,expecting perfection of society support system,desiring to achieve self-worth.Conclusions The nurses in the communities should provide better guidance for the disabled ages as well as the caregivers,for improving their living quality.

Objective To investigate the current situation of clinical practice mode of intensive care unit specialist nurses in Chongqing city, and to provide reference for the construction of standardized clinical practice mode of intensive care unit specialist nurses. Methods A self-designed questionnaire was used to investigate and analyze the 509 students who participated in the training of intensive care unit nurses in Chongqing city. Results Clinical practice time was 2 months, the practice base could meet the clinical practice needs of clinical nurses, clinical practice and assessment methods were varied, but the time arrangement was not reasonable (6.67%, 29/435), the practice content lacked the pertinence (5.52%, 24/435), the teaching method was not reasonable (2.76%, 12/435), the teacher capital was not qualified (1.38%, 6/435) and so on. Conclusions The clinical practice base and teaching staff needs to be improved, and the clinical practice pattern of intensive care unit specialist nurses is good, but the ability to recognize it is not in place. Should be guided by the needs of nurses, under the supervision of the health department, construction of training bases, training in line with the requirements of clinical teachers, strengthen the ability of students to understand, to help study the clinical application of knowledge and skills.

Objective To monitor flow rate accuracy of the infusion pump and injection pump by using evidence-based methods , and to provide the basis for management and monitoring of medical clinical devices .Methods The flow rate accuracy of 188 infusion pumps and 253 injection pumps in the hospital were monitored ,the correction between instrument using length ,maintenance fre-quency and the flow rate accuracy were analyzed .And compared the impact of flow rate accuracy affected by instruments using length and maintenance frequency .Results The flow rate accuracy of infusion pumps and injection pumps in the hospital were 47 .34% and 79 .84% ,the instrument using length had no effect on flow rate accuracy (P> 0 .05) ,and the pass yield of maintain regular was higher the irregular maintaining ,the longer of maintenance cycle the lower of pass yield (P<0 .05) .Conclusion Using evidence-based methods in medical devices safety monitoring can help deep discover potential safety hazards ,identify problems and provide scientific solutions .

Objective To check the maneuverability of the nursing safety management indicators for skilled nursing facilities.Methods A hospital based skilled nursing facility in Chongqing was chosen as the main study target while a Red Cross nursing hospital in Shanghai which operated earlier was also included as a comparison object.Combination of files reference,on-the-scene observation,manager interviews,sampling survey and institution's self-assessment,scores of indicators were calculated,and the total scores for both institutions were calculated as well.Results The institution from Chongqing marked 81 points while the other one got 86 points.Conclusions The indicator system had good maneuverability and the weight of indexes was consistent with the practical requirement of safety management.This indicator system could provide reference for standardize skilled nursing facilities' management,but still needed further revision and consummation.

BACKGROUND:A steady method that can isolate endothelial progenitor cells(EPCs) need to be explored.Studies show that the EPCs riches in bone marrow with strong reproductive activity,which can be easily obtained.OBJECTIVE:To evaluate the isolating culture and biological characterization of EPCs derived from rat marrow.DESIGN,TIME AND SETTING:The in vitro cytology experiment was performed at the central laboratory of the First Affiliated Hospital of Chongqing Medical University from August to December 2008.MATERIALS:Twenty SD rats,with two-week-old,were obtained from Experimental Animal Center of Chongqing Medical University.METHODS:femurs and tibias of rats were isolated under sterile conditions,and then the bone marrow cavity was washed by 4 ℃ 0.01 mol/L phosphate buffer(PBS).Mononuclear cells were collected from marrow suspension by density gradient centrifugation,followed by cultured with M199 culture medium containing 20% fetal bovine serum to obtained cell suspension.The cells were vaccinated into culture flask(coated with fibronectin) with the density of 1?109/L,and incubated at 37 ℃ in 5% CO2 atmosphere.MAIN OUTCOME MEASURES:Morphologic change of cells was observed by an invert microscope,the expression of CD133,CD34 as well as KDR was detected by immunocytochemistry,and the flow cytometry was used to analyze the growth curve.RESULTS:The adherent cells formed clusters that were found to be characterized by density packed round cells surrounded by spindle-likes cells.Adherent cells were identified positive for CD133,CD34 and KDR by immunocytochemistry on days 4,7,10 and 14.CD133-positive cells reduced after 10 days.Flow cytometry analysis indicated that the number of CD133/KDR double positive cells gradually increased to the maximum at day 10 and then declined.KDR-positive cells gradually increased up with the time.EPCs growth curve showed that cells proliferated fast on days 3-6.CONCLUSION:EPCs can be isolated and cultured by density gradient centrifugation from marrow suspension of SD rats.These cells exhibit the characterization of endothelial cells,and can differentiate into endothelial cells.

objective To develop long-term care demand assessment indicators for the disabled elderly in nursing home.Methods The assessment indicators were formulated based on literature review,semi-structured interviews and expert meeting.Then,Delphi technique was used to identify the indicators through two rounds of consultation among 23 experts.Results The response rates of two rounds consultation were 74.19％ and 95.65％,and the authority coefficients of experts were 0.850 and 0.905.The coordination coefficients of two levels of indicators in the second round were 0.600 and 0.269,respectively (P＜0.001).The long-term care demand assessment indicators for the disabled elderly in nursing homes consisted of 5 first-level indicators and 40 seeond-level indicators.Conclusion The longterm care demand assessment indicators for the disabled elderly in nursing home is reliable and scientific,which can provide references for further developing long-term care contents for the elderly in the nursing home and standardizing quality of long-term care.

Objective To discuss the informatization of multi hospitals. Methods Considering about the current situation of in‐formation construction to the First Affiliated Hospital of Chongqing Medical University, a set of solution was put forward based on the all in one card together with the two level information platform. Results Initial trials proved the feasibility of the solution. Con‐clusion The informatization of the multi hospitals contributes a lot to the source sharing between the regional hospitals, the im‐provement of hospital management and a better medical service.