Objective To investigate the effect of quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa on the apoptosis of Caco-2 cells and its mechanism. Methods Caco-2 cells were incubated with 3OC12-HSL for 4 h and then examined by MTT method for cytoactivities. Flow cytometry was used to analyze apoptosis rate of Caco-2 cells. Expression of apoptosis associated proteins p-p38/MAPK and NF-κB were detected by Western blot. Results After exposure to 3OC12-HSL for 4 h, cytoactivities of Caco-2 cells was reduced(P<0.05) with dose-dependent pattern, and higher dose of 3OC12-HSL leaded to increasing apoptosis rate of Caco-2 cells(P<0.05). 3OC12-HSL raised expression of apoptosis associated proteins p-p38/MAPK and NF-κB detected by Western blot. Conclusion Quorum sensing autoinducer 3OC12-HSL can effect cytoactivities of Caco-2 cells and may induce its apoptosis by enhancing the expression of p-p38/MAPK and NF-κB protein.

A high performance liquid chromatographic method for the determination of phenoxyacetic acid and 2,4-dichlorophroxyacetic acid in environnmental waters was described.Phenoxyacetic acid and 2, 4-dichioropheroxyacetic acid in sample were enriched on Sep-Pak C18 column,and methanol was employed as elutent.The shim-pack CLC ODS (150mm×6.0mm i.d.) column was used as separation column at 40°C and eluted with methanol-water (9:1,V/V)(pH 3.0) at flow rate of 1.0mL/min,the detection wavelength was 275 nm.The recoveries of the two compounds were＞98%.The detection limit was 0.04mg/L.

Objective To explore the effects of Levoeitirize dihydrochloride on interleukin-13(IL-13)and interleukin-18(IL-18)in the serum of the patients with cough variant asthma(CVA).Methods 70 cases with CVA were randomly devided into control group of 35 cases and treatment group of 35 cases.Control group was given Chlort rimeton and the treatment group was given Levocitirize dihydroehloride.The levels of IL-13 and IL-18 in the serum were measured before and after treatment.Results After treatment,the concentrations of IL-13 and IL-18 in patients in the treatment group were(46.7±17.3)ng/L and(145.2±27.1)ng/L，and those in the control group were(98.5±30.7)ng/L and(179.6±30.5)ne/L，which were significantly improved.Conclusion The treatmem of Levoeitirize dihydrochloride could improve the CVA through improving the production of IL-13 and IL-18.

Objective To explore the effects of sodium ferulate injection on CTGF, MMP-9 and TIMP-I in the serum of the patients with idiopathic pulmonary fibrosis(IPF). Methods 46 cases with IPF were randomly divid-ed into control group of 20 eases and treatment group of 26 cases. Control group was given the usual treatment and the treatment group was given the usual treatment and sodium ferulate injection. The levels of CTGF, MMP-9 and TIMP-1 in the serum were measured before and after treatment. Results After treatment,the concentration of CTGF,MMP-9 and TIMP-1 in patients in the treatment group was significantly improved than that in the control group(P < 0.05). Conclusion The treatment with sodium ferulate injection could improve the IPF through improving the production of CTGF,MMP-9 and TIMP-1.