0.45, as the normal parameters to assess left ventricular distolic filling in subjects above 40 year old.

Objective To evaluate the efficacy and safety of Qijialifei capsule,a kind of Chinese traditional medicine,as adjuvant chemotherapy for retreatment patients with smear positive pulmonary tuberculosis.Methods One hundred and sixty retreatment cases with smear positive pulmonary tuberculosis were divided into observation group (80 cases) and control group (80 cases).All patients received the therapy of 3SHRZE/6HRE.The patients in observation group took Qijialifei capsule.Then comparisons were made between two groups in the sputum tubercle bacillus conversion rate,focus improvement rate,cavity closing rate,the cellular immune function,and clinical symptom improvement.Results In 160 cases,77 cases in observation group and 78 cases in control group completed the treatment.At the end of intensified therapy period and at the end of the whole therapy the sputum tubercle bacillus conversion rate [79.2％ (61/77),92.2％ (71/77)] in observation group were significantly higher than those in control group [64.1％ (50/78),78.2％ (61/78)](P ＜ 0.05).The focus improvement rate,cavity closing rate in observation group [74.0％(57/77),26.0％(20/77)] were significantly higher than those in control group [52.6％ (41/78),11.5％ (9/78)] (P ＜ 0.05).At the end of intensified therapy period,the focus improvement rate,cavity closing rate in observation group [92.2％ (71/77),54.5％ (42/77)] were significandy higher than those in control group [76.9％ (60/78),29.5％ (23/78)] (P ＜ 0.05).By the end of the whole therapy,the number of CD3+,CD4+,CD4+/CD8+ of patients in observation group were significantly higher than those in control group (P ＜ 0.05).The disappearance of clinical symptoms in observation group was shorter than that in control group(P ＜ 0.05).The rate of side effects on Qijialifei capsule was only 1.3％(1/77).Conclusions Qijialifei capsule can improve the cellular immune function of the patients,can effectively alleviate the symptoms and can improve the sputum tubercle bacillus conversion rate,radiographic focus improvement rate and cavity closing rate.It is safe.

Objectives To test a simple method for genotyping of C.trachomatis isolates and to investigate the clinical significance of the genotypes. Methods A part of the chlamydial genome encoding the major outer membrane protein(omp1) was amplified by polymerase chain reaction (PCR). The products were digested by endonucleases to see the characteristic patterns, after silver staining on 10% polyacrylamide gels. Results The omp1 genes of 15 serovars of C. trachomatis were amplified by PCR,which generated an 871 base pair gene fragment. AluⅠ digestion of the product gave characteristic patterns for the 15 serovars,but group C presented closely similar patterns. A triple digestion with HpaⅡ, followed by HinfⅠ and EcoRⅠ, would allow the differentiation of serovars in group C. Analysis of 74 clinical isolates revealed serovars E, F, D, G as the most prevalent genital serovars in the studied populations. Serovars B, H, J were occasionally identified. A mixed infection with serovars F and D was seen in a clinical sample. No significant relationship was observed between clinical manifestations of urogenital chlamydial infections and serovars,however,serovar D was more often associated with high titer of anti chlamydial antibody than other serovars. Conclusion The omp1 genotyping technique seems to be promising for epidemiological studies.

Objective To investigate the current status of health education among nursing undergraduates,to analyze the existing problems and to bring up corresponding strategies for improving the health education ability for nursing undergraduates.Methods Totally 381 nursing undergraduates were recruited and investigated with a self-designed questionnaire.Aspects of students' cognition of health education,influencing factors of health education and the implementation of health education in clinic were investigated.Data were processed using SPSS 12.0 and percentages were calculated.Resuits 98.75％ students understood the importance of heath education in nursing work.However,systemic training courses of health education for nursing undergraduates were insufficient in both colleges and hospitals.Meanwhile,clinical teachers' ability of health education was limited.Only 15.59％students provided health education for patients independently.Conclusions Nursing colleges and practicing hospitals should strengthen health education consciousness of nursing undergraduates,enhance faculty construction and should set up related courses to train the health education skills.

Objective To explore the variation of serum D-lactic acid levels and the correlation between different intestinal viral and intestinal barrier function in children with hand, foot and mouth disease (HFMD). Methods The stools of 129 children with HFMD were collected and were detected by reverse transcription polymerase chain reaction. The children were divided into four groups according the fecal pathogens detection:enterovirus 71 (EV71) positive group, Coxsackie virus A16 (CoxA16) positive group, other intestinal virus positive group (EA positive group), and no intestinal virus group (EA negative group). Two ml venous blood and separation serum was collected in each group of children, and the level of serum D-lactic acid was detected further. Results There were 20 cases of other Coxsackie virus, and they were not grouped. Fifty-seven patients were in EV71 positive group, 26 patients were in CoxA16 positive group, 10 patients were in EA positive group, and 16 patients were in EA negative group. The level of D- lactic acid in four groups was 26.28 (17.02- 58.01), 14.43 (8.93- 20.66), 10.66 (6.64- 18.51) and 14.07 (11.83- 27.46) mg/L. The results of rank test showed the level of D-lactic in EV71 positive group was significantly higher than that in EA positive group (Z=-2.432, P=0.015) and EA negative group (Z=-3.092, P=0.002). Conclusions D-lactic acid level increases significantly in EV71 infection children and suggests that the illness is more serious and the intestinal barrier is damaged. Doctors should pay more attention to monitor and protect gastrointestinal function of children with HFMD.

AIM: To investigate therapeutic effects of recombinant human growth hormone(rhGH) on rat sepsis and its possible mechanisms. METHODS: Mean arterial pressure (MAP), levels of plasma TNF?,IL-1? and endotoxin,leukocyte count and survival rate within 1 week were determined after E. coli injection among control group,sepsis group and sepsis+rhGH group. RESULTS: (1)rhGH diminished the decrease of MAP, reduced plasma endotoxin and TNF? levels and increased neutrophil ratio in total leukocytes in sepsis rat. rhGH increased survival rate within 1 week on sepsis rat. (2)No changes were found in IL-1? level among the three groups. CONCLUSION: rhGH showed desirable beneficial effects on rat sepsis, which may attribute to: improving circulatory function; maintaining intestinal mucosa barrier, attenuating bacteria/endotoxin translocation and inhibiting the production and release of TNF?.

Objective:To investigate the change of apoptosis-associated genes in human lung adenocarcinoma cell lines A549/DDP cells,which were induced by the recombinant human interleukin-24(rhIL-24) combined with Cisplatin (DDP). Methods: Six genes expression level by GeXP genetic analysis system at the same time,after rhIL-24,DDP and rhIL-24+DDP were used to intervene in A549/DDP cells. Results:rhIL-24 could induce Bax gene,Caspase3 gene and Rb gene transcription up regulation;Bcl-2 gene and survivin gene transcription down regulation. But no regular change in the genes expression level of P53. Bax,Survivin and Rb were more obviously changed after rhIL-24 combined with DDP. Conclusion: RhIL-24 can induce apoptosis of A549/DDP cells through upregulated the genes expression level of Bax,Caspase3,Rb and down regulated of Bcl-2,Survivin.

To study the chemical constituents of Asarum himalaicum, fifteen compounds were isolated from a 70% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, and semi-preparative HPLC. By spectroscopic techniques including 1H NMR, 13C NMR, and HR-ESI-MS, these compounds were identified as 4-demethoxyaristolochic acid BII (1), aristolochic acid I (2), aristolochic acid Ia (3), 7-hydroxyaristolochic acid I (4), aristolochic acid IV (5), aristolic acid II (6), debilic acid (7), aristololactam I (8), 9-hydroxyaristololactam I (9), 7-methoxyaristololactam IV (10), (2S)-narigenin-5, 7-di-O-beta-D-pyranosylglucoside (11), 4-hydroxybenzoic acid (12), 3, 4-dihydroxybenzoic acid (13), 4-hydroxycinnamic acid (14), and beta-sitosterol (15). All of these compounds (1-15) were obtained from A. himalaicum for the first time. Among them, 1 was identified as a new compound, and compounds 3-6, 9, 12-14 were isolated from Asarum genus for the first time. Since the kidney toxicity of aristolochic acids and aristololactams has been reported, the result of this investigation suggests that it should be cautioned to use A. himalaicum as a medicine.

65% of many the Mao clean.Do contrast of clean the effect.RESULTS The Mao content is higher of many the Mao clean a product to clean effect an obvious good to content opposite and lower of; Apparatus in time processing of under the circumstance hydration meaning be not big. CONCLUSIONS The choice of exactitude clean a product,is assurance the apparatus clean quality and disinfect put out germ effect of importance factor.

Objective: To investigate effect of exogenous wild-type p53 gene on the cell growth and tumorigenicity of human gallbladder cancer cell lines. Methods: After identification of the genetic status of p53 gene of GBC-SD cell lines with the immunocytochemistry staining and the direct sequencing technique of PCR products, eukaryotic expressing plasmid pCMV-p53 was introduced by lipofectamine-mediated into GBC-SD cell lines. Growing transfected cells were selected by G418. The presence and expression of exogenous p53 gene was detected by PCR, RT-PCR and Western blot. The cellular proliferating ability was assessed using the cell growth curve and cloning assay. The xenograft in nude mice was performed to examine the effect of tumorigenicity. Results: P53 protein overexpression was showed in GBC-SD cell lines. A transversion of TAC→AAC at codon 126 of exon 5 was confirmed. PCR, RT-PCR and Western blot showed exogenous p53 gene had successfully transfected into GBC-SD cells and obtained high expression. The growth and proliferation of the cells were greatly decreased, and the tumorigenicity was significantly inhibited after transfection wtp53. Conclusion: The expression of exogenous wild-type p53 gene could effectively inhibit the growth of gallbladder cancer GBC-SD cells in vitro and in vivo.