Objective To compare the pharmacokinetics on rabbit after left gastric regional arterial infusion chemotherapy with peripheral intravenous administration.Methods 18 rabbits were seperated into 6 time groups at random and 5 Fu (50mg/kg) was infused through left gastric artery. Blood of portal vein and peripheral vein were sampled at different times. Finally all rabbits were killed and partial stomach tissues were sampled. Blood and stomach tissue were analysed with high efficacy liquid chromatography after disposed. Control group members were infused 5 Fu (50 mg/kg) by ear marginal vein and then blood and stomach tissue samples taken at different times were analysed with the same method. Results The drug concentration time curve of portal vein and peripheral vein both reached the peak at the fifth minute after drug administration in the two methods, then declined quickly and slowed down after 30 minutes, according with two house models. The drug concentration in portal vein of the experimental group was obviously higher than in the peripheral vein and also higher than in the portal vein of the controll group maintaining for a longer period. Drug concentration in stomach tissue of experimental group was 11 times of that of control group at the fifth minute and was similar after 2 hours in both methods groups.Conclusions Drug concentration in blood of portal vein and stomach tissue can maintain a higher level in a longer period with the same local effect after left gastric arterial infusion chemotheraphy showing high chemotherapeutic efficiency.

Objective The CT features of hepatic abscess (HA) were studied to differentiate form hepatocellular carcinoma(HCC).Methods The CT and operative-pathological findings of 78 cases with HA were analyied and compared with the CT appearances of a series proven cases of HCC.Results None of 25 cases had the so-called typical findings such as the marginal"double-target sign"and the even liquidizing necrotic area and containing gas zone within the lesion,53 cases had the central even liquidizing necrotic area,the marginal "Double-target sign",and containing gas zone within the lesion.Most of a typical hepatic abscess were early HA,and the precontrast CT showed nonspecific features.There were characteristic features on contrast CT.They were peripheral multicyst sign,sharp-edged sign spider-like sign,continued enhancement sign.Conclusion The CT features of HA are very valuable for the diagnosis of HA and the differential diagnosis from HCC.

OBJECTIVETo standardize the experimental procedure of the gene test for autosomal dominant cerebellar ataxias (ADCA), and provide the basis for quantitative criteria of the dynamic mutation of spinocerebellar ataxia (SCA) genes in Chinese population.

METHODSGenotyping of the dynamic mutation loci of the SCA1, SCA2, SCA3, SCA6 and SCA7 genes was performed, using florescence PCR-capillary electrophoresis followed by DNA sequencing, to investigate the variation range of copy number of CAG tandem repeat of the genes in 263 probands of ADCA pedigrees and 261 non-related normal controls. Based on the sequencing result, the bias of the CAG copy number estimation using capillary electrophoresis with different DNA controls was compared to analyze the technical detailes of the electrophresis method in testing the dynamic mutation sites.

RESULTSPCR products containing dynamic mutation loci of the SCA genes showed significantly higher mobility than that of molecular weigh marker with relatively balanced GC content. This was particularly obvious in the SCA2, SCA 6 and SCA7 genes whereas the deviation of copy number could be corrected to +/-1 when known CAG copy number fragments were used as controls. The mobility of PCR products was primarily related to the copy number of CAG repeat when the fragments contained normal CAG repeat. In the 263 ADCA pedigrees, 6 (2.28%) carried SCA1 gene mutation, 8 (3.04%) had SCA2 mutation and 81 (30.80%) harbored SCA3 mutation. The gene mutation of SCA6 and SCA7 was not found. The normal variation range of the CAG repeat was 17-36 copies in SCA1 gene, 13-30 copies in SCA2, 14-39 copies in SCA3, 6-16 copies in SCA6 and 6-13 copies in SCA7. The heterozygosity was 76.1%, 17.7%, 74.4%, 72.1% and 41.3%, respectively. The mutation range of the CAG repeat was 49-56 copies in SCA1 gene, 36-41 copies in SCA2, 59-81 copies in SCA3. Neither homozygous mutation of an SCA gene nor double heterozygous mutation of the SCA genes was observed in the study.

CONCLUSIONThe copy number of the CAG repeat in SCA genes could be calculated accurately based on the result of florescence PCR-capillary electrophoresis when limited amount of known repeat copy number controls were used. Our result supported that the notion that SCA3 gene mutation was the most common cause for ADCA, and the obtained data would be helpful for establishing quantitative criteria of the dynamic mutation of the SCA genes in Chinese.

Adolescent ; Adult ; Aged ; Ataxin-7 ; Ataxins ; Base Sequence ; Calcium Channels ; genetics ; Cerebellar Ataxia ; genetics ; Gene Dosage ; Genes, Dominant ; Genetic Variation ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Mutation ; Nerve Tissue Proteins ; genetics ; Trinucleotide Repeats ; Young Adult