OBJECTIVE To investigate the mechanism of Yifei xuanfei jiangzhuo formula （YFXF） against vascular dementia （VD）. METHODS The differentially expressed genes of YFXF （YDEGs） were obtained by network pharmacology. High-risk genes were screened from YDEGs by using the nomogram model. The optimal machine learning models in generalized linear， support vector machine， extreme gradient boosting and random forest models were screened based on high-risk genes. VD model rats were established by bilateral common carotid artery occlusion， and were randomly divided into model group and YFXF group （12.18 g/kg， by the total amount of crude drugs）， and sham operation group was established additionally， with 6 rats in each group. The effects of YFXF on behavior （using escape latency and times of crossing platform as indexes）， histopathologic changes of cerebral cortex， and the expression of proteins related to the secreted phosphoprotein 1 （SPP1）/phosphoinositide 3-kinase （PI3K）/protein kinase B （aka Akt） signaling pathway and the mRNA expression of SPP1 in cerebral cortex of VD rats were evaluated. RESULTS A total of 6 YDEGs were obtained， among which SPP1， CCL2， HMOX1 and HSPB1 may be high-risk genes of VD. The generalized linear model based on high-risk genes had the highest prediction accuracy （area under the curve of 0.954）. Compared with the model group， YFXF could significantly shorten the escape latency of VD rats， significantly increase the times of crossing platform （P＜0.05）； improve the pathological damage of cerebral cortex， such as neuronal shrinkage and neuronal necrosis； significantly reduce the expressions of SPP1 protein and mRNA （P＜0.05）， while significantly increase the phosphorylation levels of PI3K and Akt （P＜0.05）. CONCLUSIONS VD high-risk genes SPP1， CCL2， HMOX1 and HSPB1 may be the important targets of YFXF. YFXF may play an anti-VD role by down-regulating the protein and mRNA expressions of SPP1 and activating PI3K/Akt signaling pathway.

Objective:To develop the Surgical Readiness Scale for Female Patients with Stress Urinary Incontinence (SUI) and test its predictive performance.Methods:Based on the theory of "patient-centered" during the perioperative period, an initial scale was formed through literature review, semi-structured interviews, expert consultation, and pre-survey. From January 2021 to December 2022, convenience sampling was used to select 585 female SUI patients who underwent elective surgical treatment at the First Affiliated Hospital of Zhengzhou University as participants for a survey to test the reliability and validity of the scale.Results:A total of 585 questionnaires were distributed, and 560 valid questionnaires were collected, with a valid response rate of 95.73% (560/585). The Surgical Readiness Scale for Female Patients with SUI included four dimensions and 28 items. The content validity index of the scale was 0.975. Cronbach's α coefficient of the total scale was 0.909, and Cronbach's α coefficients of each dimension were 0.928, 0.822, 0.958, and 0.880, respectively. The total split-half reliability coefficient was 0.712, and the split-half reliability coefficients for each dimension were 0.921, 0.808, 0.941, and 0.841, respectively. Two exploratory factor analysis showed a cumulative variance contribution rate of 64.25%. Confirmatory factor analysis showed that the chi-square degree of freedom ratio was 1.680, the mean square root of asymptotic residuals was 0.056, the goodness of fit index was 0.840, the modified goodness of fit index was 0.811, and the comparative goodness of fit index was 0.944. The convergence validity and discriminant validity values were within a reasonable range, indicating good fit of the model.Conclusions:The Surgical Readiness Scale for Female Patients with SUI has good reliability and validity, and can be used as an effective evaluation tool for the surgical readiness of female SUI patients.

Objective:To explore the application effect of non-immersive virtual reality technology in preoperative visit of gynecological robotic surgery patients, and to provide reference for improving the preoperative visit effect in the operating room.Methods:By adopting a quasi-experimental study, a total of 80 patients who underwent Da Vinci robot-assisted gynecological surgery in the First Affiliated Hospital of Zhengzhou University were selected. The eligible patients from January to March 2022 were selected as the control group (39 cases), and the patients from April to June 2022 were selected as the observation group (41 cases). The control group used routine preoperative visit sheet and education video for preoperative visit, while the observation group used preoperative visit sheet combined with virtual reality technology for preoperative visit. The anxiety and tension, the degree of coordination of body position and the satisfaction of preoperative visit were compared between the two groups.Results:After intervention, the anxiety score, blood pressure and heart rate of the observation group patients were (38.12 ± 2.47) points, (76.37 ± 7.53) mmHg(1 mmHg=0.133 kPa), (119.20 ± 5.88) mmHg, and (79.51 ± 8.38) times/minute, respectively, which were significantly lower than the control group′s (44.56 ± 5.10) points, (82.77 ± 8.97) mmHg, (125.74 ± 8.21) mmHg, and (84.92 ± 7.48) times/min, and the differences were statistically significant ( t values were 3.04-7.25, all P<0.05). The proportion of patients in the observation group who did not require assistance and needed partial assistance and needed the full assistance of itinerant nurses in position placement were 51.22% (21/41), 41.46% (17/41), 7.32% (3/41), while those in the control group were 23.08% (9/39), 56.41% (22/39), 20.51% (8/39), and the difference was statistically significant ( Z=2.74, P<0.05). The satisfaction score of preoperative visit in the observation group was (2.66 ± 0.48) points, which was higher than (2.18 ± 0.56) points in the control group, and the difference was statistically significant ( t=-4.12, P<0.05). Conclusions:Non-immersive virtual reality technology makes the preoperative interview of Da Vinci robotic surgery more vivid, which can alleviate the preoperative anxiety of patients, improve the autonomy of patients' position placement and the satisfaction of patients with the preoperative interview.

Objective:To explore the feelings and experiences of female patients with stress urinary incontinence during preoperative self-readiness.Methods:This is a qualitative research. From September to December 2021, a total of 12 female patients with stress urinary incontinence who underwent elective surgery in the Urology Surgery of the First Affiliated Hospital of Zhengzhou University were selected by objective sampling, and semi-structured interviews were conducted. The interview data were sorted and analyzed through Braun's six-step thematic analysis.Results:A total of four themes were extracted including lack of surgical knowledge, high expectation of surgical outcome, heavy psychological burden of surgery, and high demand for external support. The lack of surgical knowledge included three sub-themes, namely, the lack of understanding of informed consent of surgery, the passive acceptance of informed consent information of surgery, and the bias of surgical information recall. The high expectation of surgical outcome included three sub-themes, namely, complete cure of urinary leakage, unrestricted physical and social activities, and getting rid of the negative emotion of "social cancer". The heavy psychological burden of surgery included two sub-themes, namely, concerns about the effects and complications of surgery and the tension of public health events. The high demand for external support included three sub-themes, namely, the need for emotional communication with medical and nursing staff, the need for experience sharing with patients, and the need for emotional support from family members and friends.Conclusions:Medical and nursing staff should pay attention to improving the surgical knowledge of female patients with stress urinary incontinence, respect their autonomy of informed consent, correctly guide patients to establish surgical expectations, and provide them with multi-dimensional emotional support, so as to promote the improvement of patients' preoperative readiness.

Objective : To establish the liver conditional knockout mouse model ofNod2gene infected byKlebsiella pneumoniae(K.pneumoniae),and to explore the role and mechanism ofNod2gene in the process of liver abscess caused byK.pneumoniaeinfections. Methods : Nod2flox/floxmice were obtained by self-crossing ofNod2flox/+mice, andAlb-Cre+mice were hybridized withNod2flox/+to obtainNod2flox/+;Alb-Cre+mice, then the above two genotypes mice were crossed to obtain liver conditional knockout mice ofNod2gene(Nod2flox/flox;Alb-Cre+) and negative control mice in the same litter(Nod2flox/flox).The genomic DNA of mice toe was extracted and amplified by polymerase chain reaction(PCR).The genotypes of offspring were identified by agar-gel electrophoresis and the livers of mice were extracted.Real-time fluorescence quantitative PCR(RT-qPCR) and Western blot were used to verify the knockout efficiency ofNod2gene in the liver.Both experimental group and control group mice were infected withK.pneumoniae,and the survival rate and pathological changes of livers were observed at different time points, and mRNA expression levels of Tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β) and C-X-C motif chemokine ligand 1(CXCL1) in the livers of experimental group and control group were detected by RT-qPCR 24 h postK.pneumoniaeinfections. Results : The expression of NOD2 mRNA in the liver ofNod2flox/flox;Alb-Cre+mice decreased, and the Western blot results showed that the expression of NOD2 protein decreased.Compared with the control group, the survival rate of mice infected withK.pneumoniaein the experimental group decreased(median survival time=60.5 h,P=0.046 9) and the liver tissue showed more serious pathological damage, furthermore the mRNA expression levels of TNF-α,IL-1β and CXCL1 in the livers of experimental group were lower than those of the control group, and the difference was statistically significant(P<0.05). Conclusion NOD2 plays a protective role in the process of liver abscess induced byK.pneumoniaeinfections.

By reviewing the concept, content, evaluation tools and significance to clinical practice of preoperative patients' preparedness for selective surgery, this paper aims to provide some reference for enriching the content of preoperative patients' preparedness and improving the surgical satisfaction and quality of life for selective surgery.

Objective To establish a scientific and reasonable evaluation index system of nursing quality for cystoscopy. Methods After 2 rounds of Delphi survey technique, get the consensus of the evaluation indicators of the initial evaluation of the 18 experts,and ultimately build a system for nursing quality evaluation index during cystoscopy. Results After 2 rounds of expert consultation, the evaluation index system based on the three level structure of the medical quality framework includes:3 first-level indicators,8 second-level indicators,33 third-level indicators. Conclusions The evaluation index system established in this study is helpful to standardize the nursing behaviors of nurses in the examination process,improve the quality of nursing service in the process of cystoscopy, and improve the accuracy and reliability of inspection.

Objective To establish a quality indicator system of nursing services for patients with urodynamics. Methods Delphi method was used in the study, and the quality indicator system of nursing services for patients was based on two rounds of consultation among 16 experts. Results The quality indicator system of nursing services for patients with urodynamics consisted of 3 first-level indicators, 8 second-level indicators and 29 third-level indicators. Experts had the same opinions on the indicators. Conclusions Nursing behavior for patients with urodynamics could be standardized based on the quality indicator system, and therefore guide nursing work and improve nursing quality. Further theoretical and empirical study is needed to verify the quality indicator system.

A new method was established for the direct, rapid and quantitative analysis of pesticide residues, dimethoate chlorothalonil and malathion by low temperature plasma ( LTP) ionization miniature ion trap mass spectrometer. The LTP ionization probe and sample inlet of ion trap mass spectrometry were enclosed in a metal cavity. With non-contact heating, the samples placed on the sample platform were desorbed into gaseous phase and ionized by LTP ionization probe. The results showed that closed ionization had an edge over the opened ionization. The quantitative analysis of 3 pesticides within the range of 0. 5-10 mg/L was realized by optimizing heating time and flow rate of air, and the relative standard deviations of signal intensity is less than 11%. LODs of pesticide, which were obtained within 5 s, were as low as several hundred pictograms. The results showed that the method could be used for the analysis of pesticide residue on green and organic fruits or vegetables.

OBJECTIVEThe non-specific antitumor immunity effect of 4-1BBL-B7-H3 gene was investigated by establishing an oral squamous cell carcinoma human peripheral blood lymphocyte-severe combined immunodeficient (SCID) mice chimeric model.

METHODSForty mice were randomly divided into five groups. All groups, except the non-immune reconstitution group (group D), had reconstructed human partial immune system. The control group (group A) was injected with Tca8113 cells. The Ad4-1BBL-B7-H3 group (group B) was injected with Tca8113 cells transfected by adenovirus containing 4-1BBL-B7-H3 gene. The empty vector group (group C) was injected with Tca8113 cells transfected by adenovirus containing an empty vector. The non-immune reconstitution group (group D) was injected with Tca8113 cells. The non-tumor group (group E) was injected with PBS. The tumor volumes in each group were measured weekly. Human IgG in blood was obtained through the tail vein and was determined by enzyme-linked immunosorbent assay. Human CD3+ and D56 lymphocytes were assessed by flow cytometry. Model animals were killed on the ninth week. Differences in the expression of the natural killer group 2 member D (NKG2D) and toll-like receptor 2 (TLR2) in tumor tissues of each group were observed by immunohistochemical method. 4-1BBL-B7-H3 gene expression in mice tumor tissues was detected by reverse transcription polymerase chain reaction (PCR) and the expressions of major histocompatibility complex 1 class related molecule (M1C) A, M1CB, and TLR2 were detected by real-time quantitative PCR.

RESULTSThe tumor volumes of group B were remarkably lower than those in the other groups (P < 0.05). Human IgG and CD3+ and CD56+ lymphocytes were detected in the peripheral blood of immune-reconstituted mice. These lymphocytes were remarkably higher in group B than those in groups A, C, and E (P < 0.05). Higher NKG2D and TLR2 expression were observed in group B tumor than those in the other groups. The stable expression of 4-1BBL-B7-H3 gene in group B was proven. The expression of M1CA, M1CB, and TLR2 were significantly higher in the group B tumor than those in groups A, C, and D (P < 0.05).

CONCLUSIONThe high 4-1BBL-B7-H3 gene expression in tumor tissues could successfully induce the proliferation of CD3+ and CD56+ lymphocytes. This expression can also directly or indirectly activate TLR2 and up-regulate the expression of NKG2D and its ligands (M1CA and M1CB), which result in an effective antitumor immune response.

4-1BB Ligand ; Adenoviridae ; Animals ; Carcinoma, Squamous Cell ; Disease Models, Animal ; Genetic Vectors ; Humans ; Mice ; Mice, SCID ; Mouth Neoplasms ; Neoplasms ; Transfection