Objective To determine the effect of thiazolidinediones (TZDs) on early retinopathy in rats with experimental diabetes. Methods In 40 rats, diabetic models were set up in 36 by one-off intraperitoneal injection with streptozotocin (STZ), and other 4 were in the normal control group. Twenty-four diabetic rats with the disease-duration of more than 6 months underwent intravitreous injection (with rosiglitazone or pioglitazone in 10 rats, respectively), and the rest 4 rats weren′t injected with drugs as the diabetic positive control group. Immunohistochemical treptomycin-avidin-biotin-complex (SABC) method, in situ hybridization of retinal vascular endothelial growth factor (VEGF) mRNA, and TdT-dUTP terminal nick-end labelling (TUNEL) were performed on the ocular paraffin section to detect the cellular apoptosis. The difference of VEGF expression and cellular apoptosis between TZDs and control group was observed and analyzed. Results The results of immunohistochemical staining and hybridization in situ were negative in the normal control group. The positive expression rate of VEGF was lower in rosiglitazone and pioglitazone group than which in the diabetic positive control group, and there was no obvious differences of positive expression of VEGF mRNA and cellular apoptosis between the 2 groups. Conclusion TZDs (rosiglitazone and pioglitazone) may inhibit the positive expression of VEGF protein in retina of STZ-induced diabetic rats to some extent, but not affect the growth of VEGF in retina.

Her-2( human epidermal growth fact or receptor 2) is a type of 185 kD across the membrane glycoprotein with tyrosine kinase activity,play an important function in the cell divisions across a cell membrane proliferation signal transduction,and finally affect biological activities of tumor cells from multiple ways,such as a tumor cell proliferation and adhesion,transfer and differentiation and other related gene regulation.Recent studies show that Her-2 overexpression rate of gastric cancer patients is about 12％ ～ 35％,and Her-2 is a gastric cancer poor-prognostic factors,while Her-2 monoclonal antibody-Trastuzumab is expected to become a new standard gastric cancer treatment for the patients with Her-2 overexpressed.

Objective:To understand the difference of prevalence rate and main symptoms of northwest dryness syndrome(NDS) of the long staying and temporary residents at Xinjiang by comparing the cotton workers in Shihezi come from mainland with inhabitant of Xinjiang,under the environment of NDS.Methods:To design questionnaire according to the Chinese medicine theories and modern epidemiology method,and carry out sampling questionnaire in cotton workers of Shihezi,Hetian,Urumqi,Yili and Leshan,then carry out syndrome differentiation with computation and statistical analysis about NDS.Results:The distributing date,syndromic types and main symptoms scores of NDS in the cotton workers in Shihezi and other areas were obtained from 3658 questionnaires.The highest attack rate of NDS on cotton workers was in Hetian.The attack rate in Sichuan was higher than that in Shizihe(P0.05).The higher scores,symptoms of DNS in Shizihe were the main symptoms of dryness syndrome,but the higher scores,symptoms of NDS in Hetian,Yili and Urumqi were the characteristic symptoms.The external-dryness score of cotton workers in Shihezi was obviously higher than that in other areas(P

Objective To conduct a comparative research on the differences and mechanisms of the effects of Con-bercept and Ranibizumab on Rhesus choroidoretinal endothelial cells ( RF/6A cells) proliferation, migration effects induced by VEGF.Methods RF/6A cells were divided into six groups, namely control group, VEGF group, Con-bercept group , Ranibizumab group , Conbercept +VEGF group and Ranibizumab +VEGF group .CCK-8 assay , Transwell chambers , Annexin V-FITC/PI double staining flow cytometry , Western blot and real-time PCR were used to detect cell proliferation , cell migration , cell apoptosis , the levels of AKT , p-AKT, P38MAPK and p-P38MAPK proteinexpressionandtherelativeexpressionofAKTmRNAandP38MAPKmRNA.Results 1)Thecellpolifera-tion decreased in a concentration-dependent manner by Conbercept and Ranibizumab treatment .The optimal con-centration and effect time of Conbercept and Ranibizumab were determined as 225 μg/mL and 24 h.2)Cell prolif-eration and cell migration were significantly decreased in Conbercept group and Ranibizumab group , but meaning-fully increased in VEGF group .Compared with VEGF group , Conbercept+VEGF group and Ranibizumab +VEGF group decreased .3 ) Cell apoptosis decreased in VEGF group , but increased in Conbercept and Ranibizumab group.Compared with VEGF group, cell apoptosis increased in Conbercept and Ranibizumab+VEGF group. 4)There was no differences in the expression of AKT and P38MAPK among groups.The expression of p-AKT, p-P38MAPK, AKT mRNA and P38MAPK mRNA were down-regulated in Conbercept and Ranibizumab group , while up-regulated in VEGF group.The expression of p-AKT, p-P38MAPK, AKT mRNA and P38MAPK mRNA were down-regulated in Conbercept and Ranibizumab +VEGF group .Conclusions Conbercept and Ranibizumab inhibit cell proliferation , migration and related protein expression , but accelerate cell apoptotis .Nevertheless , there is no statistical significance between the impacts of Conbercept and Ranibizumab on the cells .

Using a spectral analysis of heart rate variability (SAHRV) in supine and standing positions were performed in a group of 65 patients with migraine and in a control group of 50 healthy volunteers in order to quantitative evaluate the function of autonomic nervous system(ANS). The result revealed that the integrals of middle frequency bands of amplitude spectra of standing patients were smaller than that of controls (P

Contents of laboratory diagnosis is complex.Traditional teaching method cannot arouse students' interest in study and the teaching effect is poor. Teachers should choose different teaching emphasis for students in different specialty,increase and decrease theory teaching contents according to the demands of students' future actual work,introduce case analysis,train students' capability of clinical logical thinking,enhance students' perceptual knowledge of theory through the training of experiment skills and using PBL teaching method to make students truly participate in teaching course so as to arouse their enthusiasm,These reforming measures will benefit the improvement of teaching quality.

Objective To evaluate the security and the reliability of ligating the cystic duct and the cystic ar- tery with common silk suture as the substitute for titanic or biological clamps in laparoscopic cholecystectomy (LC) us- ing the suture and knot instrument developed by the authors. Methods One hundred and two laparoscopic chole- cystectomies were performed by the authors using the instrument. Results All patients were discharged in the post- operative fourth or fifth day without any complication. Conclusion The instrument used for LC was safe and reliable.

Objective: To study the nature and flavour of LIU Wan-su’ drug application and the tendency of cold or warm in his prescription; analyze LIU Wan-su’s medical sect by the index of prescription metrology. Methods: Combining the traditional documents with prescription metrology, selecting three doctors before or after LIU Wan-su in Jin and Yuan Dynasty as comparison to expose the characteristic of prescription metrology in his prescription. Results: Wen tonic frequency of each prescription on average, LI Dong-yuan(1.10),ZHU Dan-xi(0.97), LIU Wan-su (0.77), ZHANG Zi-he(0.65); Han tonic frequency of each prescription, LI Dong-yuan (1.34),ZHANG Zi-he (1.00), LIU Wan-su (0.94), ZHU Dan-xi(0.80);Cool and warm drug ratio of four sects in Jin and Yuan Dyanstay, ZHU Dan-xi (0.97),LI Dong-yuan(0.83), LIU Wan-su (0.82), ZHNAG Zi-he (0.58).Cool and warm prescription ratio, ZHANG Zi-he(1.50), LIU Wan-su (0.87), LI Dong-yuan(0.76), ZHU Dan-xi (0.50).Conclusion: As the exponent of "theory of fi re and hot", it will be better to classify LIU Wan-su as " Hejian sect" since his drug application was not limited within cold and cool. To some extent, it is more important to judge a Chinese traditional doctor by syndrome identifi cation rather than only by his drug frequency.

Fluorescence imaging of animal in vivo is to tag cell or DNA with fluorescence reporter gene, and then detect the fluorescence using sensitive optics apparatus. With this system, researcher can observe tumor growth, metastasis and angiogenesis in vivo, and reliable information for tumor diagnosis and treatment will be provided for researcher.

Near-infrared technology can be used as an auxiliary means for the early diagnosis of cancer and for surgical resection of tiny lesion that can not be discriminated by naked eyes,but it can not satisfy the purpose when used alone.Thus,we have developed an integrated technique by combining near-infrared and other technologies,and extended the scope of its application.This article reviews the advances of several integrated techniques applied to tumor research,including double labeled technology of nuclide and near-infrared,the combination of near-infrared and fluorescence resonance energy transfer,the combined use of near-infrared and endoscopy,and the integration of near-infrared with carbon nanotubes.