Objective To observe the effect of treating neonatal jaundice with Qing Kai Ling injection plus photoradiation. Methods 583 cases were randomly recruited into a control group and a treatment group.342 cases in the treatment group received Qing Kai Ling injection plus photoradiation treatment, while 241 cases in the control group received only photoradiation treatment. TBIL, DBIL regression time of jaundice and symptom improvement were observed in both groups. Results After the treatment, TBIL and DBIL's level in the treatment group was lower than the control group (P< 0.05) . symptoms improving in the treatment group was also significantly better (P=0.0087) . The recovery time for the treatment group was shorter than the control group (P= 0.0068). Conclusion Qing Kai Ling injection plus photoradiation is good in treating neonatal jaundice.

Objective:To study the role of serum chemokines and their receptors expression in pathogenesis of recurrent genital herpes(RGH).Methods:Serum levels of interleukin 8(IL 8),interferon ? inducible protein 10 (IP 10),monocyte chemotactic protein1(MCP 1),macrophage inflammatory protein 1?(MIP 1?),regulated upon activation,normal T cell expressed and secreted factor(RANTES) and macrophage derived chemokine(MDC)were determined by enzyme linked immunosorbent assay in 20 patients with RGH and 30 normal controls, meanwhile, a two color immunofluorescent staining of chemokine receptors CCR2,CCR5,CXCR1 and CXCR3 on peripheral blood CD3 +T lymphocytes of patients with RGH were analyzed by flow cytometer.Results:Serum levels of RANTES were lower in patients with RGH than that in controls(P

Objective To study the roles of phenotype switch of HPV16 antigen-specific CD8~+ cy- totoxic T cells (CTLs) in peripheral blood as well as the cellular immune response of human T lympho- cytes to viral peptides in the pathogenesis of condyloma acuminatum.Methods HPV16E7~(11-20) (HLA-A*0201/YMLDLQPETT) antigen-specific CTLs were stained with fluorescent-labeled recombinant MHC class I-peptide pentamer complex,and co-stained with CD69 (activated CTL) and CD45RO (mem- ory CTL) surface markers,in peripheral blood from 10 patients with condyloma acuminatum and 15 normal controls.The CTLs were quantitated by flow cytometric analysis.Meanwhile,peripheral blood mononuclear cells of 13 healthy donors were incubated for 7 days with HPV16E7_(11-20) peptide,recombinant human inter- leukin 7 and interleukin 2 to yield antigen-specific CD8~+ CTLs and their other surface markers.An irrelevant peptide,HBVcore_(18-27) (FLPSDFFPSV),was used as an isotype control.Results The percentages of periph- eral blood HPV16E7 antigen-specific CD8~+CTLs,activated CTLs and memory CTLs of total CD8~+ T cells were increased significantly in patients with condyloma acuminatum (0.76%?0.43%,0.36%?0.20%,and 0.33%?0.15%,respectively) than those in normal controls (0.24%?0.07%,0.17%?0.05% and 0.15%?0.06%,respectively) (P＜0.01,P＜0.05,and P＜0.01,respectively).For T cells in vitro stimulated with viral peptide for 7 days,the percentages of peptide-specific CD8~+ CTLs,activated CTLs and memory CTLs were significantly higher (0.75%?0.16%,0.35%?0.15% and 0.33%?0.18%,respective- ly) in comparison with those in non-stimulated group (0.24%?0.06%,0.16%?0.03% and 0.13%?0.04%,respectively ) (P＜0.001,P＜0.01 and P＜0.01,respectively ).Conclusions These results,in vivo and in vitro,demonstrate that the proliferation of CD8~+ T cell clones induced by HPV infection could generate antigen-specific CD8~+ CTLs,causing a highly efficient and specific killing of viral-infected target cells directly,which may play an important role in antiviral T cell-mediated immune response.

Objective To investigate peripheral blood dendritic cells (DC) responding to herpesvirus infection in the patients with recurrent genital herpes and the expression of immune molecules on mature DC induced by the cytokines in vitro. Methods Using three-color flow cytometry, we studied the phenotype expression of CD11c, CD123, CDla, CD80, CD86, CD40 and CD83 on the two subsets of DC(CD11c+DC and CD123+DC) in peripheral blood. The monocytes isolated from the blood were cultured with recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-?) from 20 patients with recurrent genital herpes (RGH) and 18 healthy controls. Results The number of CD11c+ DC in the peripheral blood increased significantly in the peripheral blood cells of RGH patients (2.54% ? 1.19%) in comparison with that of healthy controls (1.77% ? 0.83%) (P 0.05). Conclusions Peripheral blood CD11c+DC activated by uptaking viral antigens in the patients with RGH results in a high level expression of co-stimulatory molecules on DC and enhance antigen-presenting functions, which is essential for intiating antiviral immune response directly by T lymphocytes.

Objective To study the role of serum chemokines and their receptors in the pathogenesis of condyloma acuminatum(CA).Methods Serum levels of interleukin8(IL-8),interferon?-inducible pro-tein10(IP-10),monocyte chemotactic protein1(MCP-1),macrophage inflammatory protein1?(MIP-1?),reg-ulated upon activation,normal T cell expressed and secreted factor(RANTES)and macrophage-derived chemokine(MDC)were determined by enzyme-linked immunosorbent assay(ELISA)in30patients with CA and30normal controls.Meanwhile,chemokine receptors,CXCR1,CXCR3,CCR2and CCR5on peripheral blood CD3 + T-lymphocytes from20patients with CA were analyzed by flow cytometry with two-color im-munofluorescent staining.Results Serum levels of IP-10and MIP-1?were significantly higher in CA patients than those in controls(P

Objective:To investigate the expression of special marker for activation on peripheral blood T lymphocytes in patients with condyloma acuminatum(CA) and its significance.Methods:Immunofluorescent three color flow cytometry was used to study the expression of CD69 and HLA DR on T lymphocytes in 30 patients with CA and 31 normal controls. Results:Expression of CD69 on CD3 + T cells were significantly higher in patients (6 63%?3 13%) than that in controls (5 12%?1 64%, P

Objective With eukaryote expression vector pEGFP-C2, to establish the HepG2 cell line which may stably express the P22e of hepatitis B virus (HBV), and may be used to study the existed relationship between HBVP22e and hepatitis B. Methods HBVP22e cDNA obtained by PCR from HBV adr subtype 1.2 copies genome plasmid p3.8II was inserted into universal vector pMD18-T for identification. The plasmids were transfected into HepG2 cells via liposome, while EGFP-HBVP22e was analyzed by Western blot, and observed with fluorescence microscope in HepG2 cells. Results Expression vectors of recombinant pEGFP-C2HBV P22e were constructed and expressed steadily in HepG2 cells. Conclusion The results can be used to explore biological activity of HBVP22e in hepatitis B.

Objective To investigate the possible roles of cellular immunosuppression induced by phenotypic and functional changes of peripheral CD4+CD25+ regulatory T cells (Tregs) in the pathogenesis of condylomata acuminata. Methods Three-color flow cytometry was performed to examine the expression of transcription factor Foxp3 in, along with several inhibitory membrane molecules, i.e. cytotoxic T lympho-cyte associated antigen-4 (CTLA-4), glucocorticoid-induced TNF receptor family-related gene (GITR) and programmed death-1 (PD-1) on peripheral CD4+CD25+ T cells from 46 patients with condylomata acuminata and 43 normal human controls. Meanwhile, high purity of CD4+CD25+ T cells were isolated from peripheral blood using imrnunomagnetic beads, and stimulated to produce intracellular suppressor cytokines such as interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta), which were detected by flow tyro-metric analysis. Results The number of peripheral CD4+CD25+ Foxp3+ Tregs increased significantly in patients with condylomata acuminata than that in the normal controls (7.37% ± 2.43% vs 5.96% ± 2.09%,P ＜ 0.001). The expressions of CTLA-4 and PD-1 were 1.86% ± 1.13% and 2.41% ± 1.12%, respectively,in the patients, which were significantly higher than those in the normal controls(1.36% ± 0.90% and 1.70%± 0.97%, P ＜ 0.05 and 0.01, respectively). The number of TGF-beta-positive CD4+CD25+ T ceils from peripheral blood were increased in patients than that in, the controls (1.57% ± 0.91% vs 0.78% ± 0.24%, P ＜0.001). Conclusions Human papillomavirus infection can induce the activation and proliferation of CD4+CD25+ Tregs, enhance the expression of negative costimulatory molecules and secretion of suppressor cytokines, and inhibit antiviral immune response through multiple mechanisms.

Although the concept of united airway disease has been widely accepted, most scholars emphasize only the effect of rhino-sinusitis while ignoring the pharyngeal factors to the lower airway, especially to the allergic pharyngitis (AP), which still lacks enough awareness. First of all, absence of unified diagnostic standard leads to the lack of epidemiological data, which, results in doctors' personal experience but no guideline in treatments. In addition, it is still not clear that the role of AP in the allergic airway diseases and its relationship with asthma. However, the number of patients with AP has been increasing obviously in daily clinic practice. Combined with the previous observation, this paper does a systematic review about the clinical problems of AP, expecting to give a hand to the clinical diagnosis and treatment of AP.
Asthma ; Humans ; Pharyngitis ; diagnosis ; therapy ; Rhinitis, Allergic, Perennial ; diagnosis ; therapy ; Sinusitis

AIM: To evaluate the therapeutic effect of Jianing Decoction (Rhizoma sparganii, Rhizoma curcumae, Radix bupleuri, Fructus schisandrae chinensis, Spuama manis, Radix polygoni multifrori, Spica prunellae, Flos chrysanthemi indici, Fructus ligustri lucidi) (JN) on autoimmune thyroiditis. METHODS: An animal model of experimental autoimmune thyroiditis (EAT) in rats was developed by using thyroglobulin of porcine (PTg). These animals were divided into JN group, triptolide (TP) group and control group. Pathological changes were observed in the thyroid tissues and serum thyroid peroxidase antibodies (TPOAb) and thyroglobulin antibodies (TGAb) were determined by the method of radiorimmuoussay. RESULTS: The serum TGAb and TPOAb were significantly higher in EAT model group than those in normal group (P