Objective To observe the mechanism of action of different extracts ofTangwang Mingmu Granules on high glucose induced VEGF and IL-1α gene and protein expressions in vascular endothelial cells.Methods Human umbilical vein endothelial cells EA.hy926 were divided into six groups: blank, high glucose,Tangwang Mingmu Granules, extract 1 (glycoside and flavonoid), extract 2 (organic acid and polysaccharides) and extract 3 (alkaloids) groups. High concentration glucose was used to establish the high glucose model in EA.hy926 cells. The expressions of VEGF and IL-1α mRNA were detected by semi-quantitative RT-PCR. The contents of VEGF and IL-1α protein were tested by ELISA.Results The gene expression and protein levels of VEGF and IL-1α were significantly up-regulated under the high glucose condition (P<0.05). However, the above indicators were significantly reduced after the treatment ofTangwang Mingmu Granules. The activity of different parts ofTangwang Mingmu Granules was as follows: extract 1> extract 3> extract 2.Conclusion The action intensity of glycosides and flavonoids, alkaloids, organic acids and polysaccharides on VEGF and IL-1α expression inTangwang Mingmu Granules weakens in sequence.

Objective To realize the simultaneous control of two laboratory instruments by a computer. Methods On the basis of analysing the mode of communication between computer and laboratory instrument, the author expanded the computer's COM interface through the USB / COM converter. Results The author realized a computer controlling MP280 CLIA chemiluminescence analyzer and anthos 2010 enzyme tester at the same time. Conclusion User can use computer's USB interface and USB / COM converter to achieve controlling multiple instruments and equipments at the same time, as long as the communication interface of equipment is open. This method has preferable potential application.