OBJECTIVE: To summarize the recent progress and clinical application of percutaneous coronary intervention and to seek the direction of new developments.METHODS: A computerized online database of Pubmed was searched to identify articles published from May 2000 to February 2009 with the key words of "drug eluting stents, peroutaneous coronary intervention, and coronary disease". There were 154 articles were retrieved by computer, by reading tities and Abstract s, 27% literatures were remained after excluding 74 irrespective papers and 53 repetitive studies.RESULTS: An ideal drug-eluting stent (DES) was comprised of a platform, a drug carrier vehicle and a pharmaceutical compound in harmony with each other. With the ongoing development of DES materials and drugs, more effective DES was introduced in the practice. Recently, clinical data on DES encourage the interventional cardiologist to use DES in more challenging coronary lesions, such as chronic total occlusions, complex lesions and multi-vessel lesions. However, the safety and efficacy of DES need further attention. Therefore, novel strategies including bioabsorbable stents, and pro-healing agents coated stents were promising.CONCLUSION: The development of percutaneous coronary intervention is a breakthrough in intervention cardiology that brings great benefit to patients with coronary disease, especially for restenosis and revascularization. Nevertheless, more endeavour will be necessary to create PCI with high efficacy as well as low risk, and safety and effectiveness of PCI in patients with ST-segment evaluation myocardial infraction and stent fracture prevention need further study.

Objective To investigate whether panax notoginseng saponins(三七总皂苷,PNS) can inhibit the activity of lung nuclear factor-?B(NF-?B) and ameliorate the lung pathologic injury in rats with acute necrotizing pancreatitis(ANP).Methods Thirty Sprague-Dawley(SD) rats were randomly divided into three groups: sham operation group(group sham),ANP group(group ANP),PNS preconditioning group(group PNS).There were 10 rats in each group.Rat ANP model was induced by retrograde injection of 5% sodium taurocholate to pancreatic duct(1 ml/kg).The rats in the PNS preconditioning group were given by intraperitoneal injection of 50 g/L PNS(1 ml/kg) one hour before model establishment,and those in the other two groups were given 0.9% physiological saline(1 ml/kg) one hour before the operation.Put the rat in each group to death at 6 hours after the respective management was made,and the pulmonary tissues were taken to detect the activity of NF?B by immunohistochemistry and the wet/dry weight ratio of the lung tissue was measured.Results At 6 hours after the model was established in the ANP group,the NF-?B positive cell number(70%-100% positive cells,7 vs.0) and the positive unit((72.52?7.63)PU vs.(33.09?4.75)PU) in the lung tissue were remarkably increased in comparisons with those in the sham group(both P

Objective To summarize the experience of microsurgery for repair on urethrovaginal and vesicovaginal fistula. Methods Five cases of complex urethrovaginal or vesicovaginal fistula underwent repair procedure with thin suture under surgical magnifier.The defect of vesical neck was sutured in several layers and the urethra was renovated by using vaginal wall tissue. Results The operations were successful in all the 5 cases with no obvious incontinence.Their course of coition was not influenced. Conclusions Suturing the defect of the vesical neck in several layers can restore the function of the sphincter.Microsurgery may align the tissue well and cause less tissue reaction,thus it is beneficial to the concrescence of the fistula.

Objective To improve the diagnostic level of on pelvic nodal metastases of prostate cancer. Methods Thirty-three cases of prostate cancer underwent instep lymphangiography to find out if there were pelvic nodal metastases.The results of lymphangiography were compared with the pathologic results of fine needle aspiration biopsy of lymph nodes. Results In the 9 cases testing positive for nodal metastases 7 were identified by cytology of fine needle aspiration biopsy and 6 were confirmed by pathology;while in the 24 cases with negative results,4 were false negative(17%). Conclusions The lymphangiography plays an important role in diagnosis of nodal metastases of prostate cancer.Fine needle aspiration for suspected lymph nodes can minimize false positive result,nevertheless there is still false negative result in lymphangiography.

Parkinson’s disease (PD)is one of familiar neurodegenerative disorder. The pathogenesis of PD,up to now,has not been fully understood. Although heredity,aging and environmental factors were involved,the combination effects of these factors were much concerned. PD animal model is an indispensable tool for exploring the mechanism,neuro-protective drugs and therapy strategies. Some kinds of animal models were introduced in this paper. MPTP mouse model is the most important and most universal use.The consideration of the effect of multiple risk factors may be the start of new era in the research field of PD animal models.

Objective To evaluate the effect of omega-3 polyunsaturaed fatty acid on liver function, donor's T lymphocytes and acute rejection following orthotopic liver transplantation in rats. Methods Orthotopie liver transplantation from BN (RT1 n) to Lewis was performed. Normal saline (NS, n = 6), parenteral nutrition (PN, n = 6) or parenteral nutrition and Omega-3 fatty acid (OM, n = 6) was given through internal jugular vein catheter respectively beginning the first day for consecutive 6 days. At 7thday, the liver function and pathological changes of the transplant liver were observed. The percentage of CD4+ , CD8+, CD4+ CD25+ and CD8+ CD28- T lymphocytes were analyzed by flow-cytometry. Results In OM group the CD4+, CD8+ , CD4+ CD25+ and CD8+ CD28- T lymphocytes significantly decreased, compared with NS (t = 4.28, P < 0.01 ; t = 2.63, P < 0.05 ; t = 2.59, P < 0.05 ; t = 8.86, P <0.01)or PN group(t =8.06, P<0.01; t =3.57, P<0.01; t =5.35, P<0.01; t =7.98, P<0.01). The ratio of CD4+ to CD8+ significantly decreased in OM group compared with NS group and PN group(t = 6.25, P<0.01; t=4.92, P<0.01). RAI (rejection activity index) in NS group, PN group and OM group was 7.17 +0.98, 6.17±0.75 and 4.33±0.52, respectively. The rejection grade in OM group was siguificanfly alleviated than that in NS group and PN group(t =6.25, P <0.01;t =4.92, P <0.01). Conclusion Omega-3 fatty acid inhibits T lymphocytes, especially help T lymphocytes, alleviates acute rejection. The suppressor T cell and the regulatory T cell were not responsible for the alleviation of the rejection.

Objective To investigate the relationship between tumor metastasis-related Rac1 mRNA expression levels and gastric carcinomas metastasis, and to investigate the significance of Rac1 as a tumor marker for the evaluation of gastric carcinomas metastasis and distinguish between benign and malignant lesions. Methods This experiment used fluorescence quantitative RT-PCR TaqMan probe technology, chose Rac1 target gene fragment, which was cloned into the pET-20b (+) vector, constructed recombinant plasmid, and established the Rac1 mRNA fluorescence quantitative RT-PCR standard curve. And then the Rac1 mRNA levels were detected in 52 cases of gastric carcinoma tissues,52 cases of para-carcinoma tissues and 12 cases of benign gastric disease tissues. Its association with the metastasis of gastric carcinomas was analyzed. Results The positive rates and levels (median, P25-P75) of Rac1 mRNA were 100. 0% ,7.41 ×105 (3. 50 × 105-4. 36 × 106) copies/μl in gastric carcinomas, 46. 2% ,0(0-1.73 × 104) copies/μl in parscarcinoma tissues, 33. 3%, 0(0-3.55 × 103) copies/μl in benign tissues. The positive rates and leves(x2 =43.16,x2Xk-w = 64. 19, P ＜0. 01)among the three groups were significantly different. When the critical value of Rac1 mRNA level was 1.73 × 104 copies/μl determined by ROC, the sensitivity and specificity were 88. 5% and 100% respectively. When the critical value of Rac1 level was 7.49 × 105 copies/μl, the sensitivity and specificity were 57.9% and 78. 6% respectively. ConclusionThe Rac1 mRNA level detected with fluorescence quantitative RT-PCR has reference value to distinguish between benign and malignant lesions and evaluate gastric carcinoma metastasis.

Objective:To study the protective effect of Ginkgo biloba L.extract(EGB) on PC12 cell damage induced by rotenone and 1-methyl-4-phenylpyridium(MPP +). Methods: PC12 cells damage model was induced with different concentrations of rotenone and MPP + and were treated with EGB . Then cell morphology was observed; survival rate and ultracellular dopamine (DA) level were determined by MTT assay and ELISA assay, respectively. Results: MPP + -induced damage was attenuated by pre- or co-treatment with EGB at 0.35,0.70 and 1.40 mg/ml, while rotenone-induced damage was attenuated with EGB only at 1.40 mg/ml.The DA concentrations of extracellular at 2 mmol/L MPP + (6.875?0.201) ng/ml and 10 ?mol/L rotenone (5.321?0.167) ng/ml increased to ( 7.595?0.139 ) ng/ml (P

Objective To compare the performances of Monaco and Pinnacle treatment planning systems (TPS) for volumetric modulated arc therapy (VMAT) optimization regarding lung cancer.The TPS was compared in terms of dose distributions,treatment delivery parameters and quality control results.Methods For 20 patients,including 10 cases of left lung cancer and l0 cases of right lung cancer,two VMAT plans were generated for each case:one with Monaco 3.0 TPS and the other with Pinnacle 9.2 TPS.Two plans were compared among plan dosimetrie distribution.conformity index and homogeneity index of the targets,the average dose,maximum dose,minimum dose and interested dose volume histograms of organs at risk(OAR,and delivery time and MUs of the therapy plans,the accuracy of treatment plans dose verification.Results Monaco provided better PTV coverage than Pinnacle (t =5.927-12.034,P ＜ 0.05) except Dminof PTV.Monaco had a worse sparing effect on lung than Pinnacle (t =3.545-7.485,P ＜0.05) except V10of diseased side lung and V5of total lung.In addition,Monaco had a better sparing effect on heart(t =2.836-4.011,P ＜0.05).Monaco had fewer delivery time(t =9.780,P ＜0.05)and MUs(t =5.304,P ＜0.05)of the therapy plans,and the pass rate of QA was better than Pinnacle(t =4.937,P ＜ 0.05).Conclusions For lung cancer patients,VMAT treatment plans obtained with Monaco and Pinnacle could offer clinically acceptable dose distributions.Pinnacle might have a better sparing effect on lung and fewer delivery time and MUs of the therapy plans.Monaco had a better PTV coverage and heart sparing.Moreover,Monaco had better accuracy of treatment plans dose verification.