Objective To study the effects of different levels estradiol on inhibitory of tamoxifen on human mammary cancer cells(ER+) in vitro.Methods Estrogen receptor(ER)-positive MCF7 human breast cancer cell line was treated by the same level of tamoxifen and different levels of estradiol in vitro.Cell proliferation was evaluated by MTT assay.Results E_2 at concentrations between 1? 10~(-12) mol/L to 1? 10~(-7) mol / L significantly stimulated the growth of MCF-7.TAM(10 ?mol/ L) inhabited the growth of MCF-7 significantly.E_2 at different levels may influence inhibitory of tamoxifen on MCF-7 cell lines.E_2(1?10~(-8) mol/L) makes inhibitory of tamoxifen on MCF-7 cell lines valueless.Conclusion E_2 is the risk factor of breast cancer,and the concentration around breast cancer cells may influence the effects of TAM.

Objective To understand the effect of estradiol in different concentrations on proliferation of diverse mammary primary cells in vitro. Methods The primary cells of cancer tissue, the adjacent tissue to tumors and normal mammary tissue from patiens with breast cancer were obtained using collagenase digesting method. All the tissue samples were cultivated in vitro, and were given estradiol in different concentrations. The effect of estradiol on the proliferation of those primary cells was measured by MTT. Results Estradiol remarkedly promoted the proliferation of primary cells of cancer tissue and peritumor tissue in vitro, whose ER expression were positive. Whereas, the promotion effect of estradiol on the proliferation of normal mammary primary cells was relatively weak, and there was no correlation between the promotion effect with the expression of ER in cancer tissue. Conclusion The risks of occurrence and relapse of breast cancer would increase significantly when the concentration of estradiol is no less than 103 pmol/L in vivo.

Objective To investigate the methods of isolation,culture,identification and labeling of mesenchymal stem cells(MSCs) in vitro and lay a foundation for further study on intervention of MSCs on immunologic rejection of organ transplantation. Methods MSCs were isolated and cultivated by adherent methods . The expressions of CD90 and CD45 of cells were analyzed by using flow cytometry in order to identify MSCs.The third generation of MSCs were labeled by DAPI,the labeling efficiency was detected.Results Primary cultured MSCs adhered to plastic surface within 48 h and reached 90% confluence within 7-10 d .Flow cytometry showed that the positive rates of CD90 and CD45 of MSCs at third generation were 99.8% and 6.8%. MSCs expressed CD90 but no CD45.All of the MSCs after labeling by DAPI showed blue fluorescence by immunofluoroscope. DAPI labeling was sensitive and highly efficient to MSCs.Conclusion Adherent method is simple and easy to isolate and cultivate MSCs and it can serve as a routine method.DAPI labeling can be used as a efficient method to label MSCs.

OBJECTIVETo compare the perioperative complications between Ivor-Lewis approach and McKeown approach in minimally invasive esophagectomy and gastric tube reconstruction for the treatment of middle and lower thoracic esophageal cancer.

METHODSRetrospective analysis of clinical data was performed on 288 patients with middle and lower thoracic esophageal cancer who underwent completely minimally invasive esophagectomy by one surgical team in Fujian Medical University Union Hospital from December 2010 to March 2014. Among the 288 patients, 103 patients underwent combined laparoscopic and thoracoscopic esophagectomy and intrathoracic esophagogastric anastomosis using a transoral anvil(Orvil)(Ivor-Lewis group, 2-incision) and 185 patients underwent combined laparoscopic and thoracoscopic esophagectomy and cervical anastomosis(McKeown group, 3-incision). Patients were stratified by surgical approach and perioperative outcomes were compared between the two groups.

RESULTSThere were no statistical differences between two groups in intra-operative blood loss, conversion to open, extubation time, time to resume oral intake, postoperative hospital stay, the median number of lymph nodes resected. The operation time of Ivor-Lewis group was significantly shorter than that of McKeown group [(283.4±32.0) min vs. (303.6±43.7) min, P=0.003). The hospital cost of Ivor-Lewis group was significantly higher than that of McKeown group [(76 492±18 553) yuan vs. (68 923±17 331) yuan, P<0.01]. There were no statistical differences between two groups in chylothorax, delayed gastric emptying, atrial fibrillation, postoperative bleeding, admission to ICU, short-term postoperative mortality (P>0.05). The total postoperative complication morbidity of Ivor-Lewis group was significantly lower than that of McKeown group(16.5% vs. 31.4%, P<0.01). Ivor-Lewis group had lower pulmonary complication(8.7% vs. 25.9%, P<0.01), anastomotic leakage(1.9% vs. 13.0%, P<0.01), anastomotic stricture (0% vs. 4.9%, P<0.05), recurrent laryngeal nerve injury(1.0% vs. 7.0%, P<0.05).

CONCLUSIONIvor-Lewis approach is associated with less postoperative complications, but higher cost as compared to McKeown approach in the treatment of middle and lower thoracic esophageal cancer.

Anastomosis, Surgical ; Anastomotic Leak ; Blood Loss, Surgical ; Esophageal Neoplasms ; surgery ; Esophagectomy ; methods ; Humans ; Laparoscopy ; Length of Stay ; Minimally Invasive Surgical Procedures ; methods ; Operative Time ; Postoperative Complications ; Reconstructive Surgical Procedures ; methods ; Retrospective Studies

Objective To investigate the role of airflow parameters of some specific examinations in voice function assessment.Methods The s/z ratio, pulmonary function and phonatory aerodynamic parameters were measured in subjects with benign vocal fold lesions and with normal voice.The effect of treatment in subjects with benign vocal fold lesions was evaluated with the phonatory aerodynamic parameters.Results The value of s/z ratio in the disease group was higher than that in the normal group (P ＜ 0.05).The value of PEF was significantly different between the disease group and the normal group fur male(P ＜0.05).MFR, MPT, PTF, SGP, PTP, VE were significantly different between the disease group and the normal group(P ＜0.05).MFR, MPT, PTF, SGP, PTP of the disease group after surgery for both sex were significantly different from before surgery (P ＜ 0.05).The disease group was subdivided into two groups through stroboscopic examination before and one month after surgery: the worse group (with some functional laryngeal abnormality, or organic abnormality except benign vocal fold lesion)and the better group.PTF, PTP, SGP, VE were significantly different between the worse group and the normal voice group.There was almost no significant difference for aerodynamic parameters between the better group and the normal voice group (P ＞ 0.05).There was no significant difference between the worse group after 8 weeks'voice training and the normal voice group (P ＞ 0.05).Conclusions s/z ratio, aerodynamic parameters (MFR, MPT, SGP, PTF, PTP, VE) are valuable for the diagnosis and assessment of the voice disorders.Aerodynamic parameters are sensitive to the change of glottal function during the treatment.Voice training can increase the glottal function of patients after laryngeal microsurgery.

OBJECTIVETo investigate the possession of occupational health examination qualification among occupational health examination institutions in Zhejiang province, China and the application of GBZ188-2007 "Technical specifications for occupational health surveillance" among related government departments, and to provide a basis for revising and improving the specifications.

METHODSA questionnaire survey was carried out among occupational health inspection agencies, health supervision authorities, and enterprises of different sizes, which possessed provincial-level occupation health inspection qualifications and had engaged in occupational health examination for more than 2 years. The investigation included awareness, using frequency, and revision suggestion about GBZ 188-2007 "Technical specifications for occupational health surveillance".

RESULTSMedical institutions and centers for disease control and prevention (CDCs)showed a high rate of occupational health examination qualification possession in chemical substances, dust, noise, and high temperature and a low rate of qualification possession in physical factors, biological factors, special operations, and other aspects. These institutions showed high awareness of chemical substances, dust, and physical factors in GBZ 188-2007 "Technical specifications for occupational health surveillance" and low awareness of other factors. Medical institutions, CDCs, and provincial and municipal health supervision authorities used these specifications more frequently than county-level authorities and enterprises. More than 80% investigated units believed that the following issues required further optimization: occupational health care, emergency health examination regulations, classification of occupational health inspection reports, and special operation monitoring. It is consistently stressed that for exposure to chemical compounds that may bring harm to various organs of the body, explicit specifications should be made concerning health examination before, during, and after service. Conflicting opinions existed concerning the following issues: satisfaction with GBZ 188-2007 specifications, whether to add contents for care of personnel exposed to biological factors (such as forest encephalitis), the threshold of noise intensity to perform health care, and whether to add health care for exposure to compounds such as benzene and phenols.

CONCLUSIONThe occupational health inspection agencies in Zhejiang province have a high rate of qualification possession in health examination concerning chemicals, dust, noise, and high temperature. The medical institutions, CDCs, and health supervision departments above the county level often use the specifications at work. It is consistently recommended that more kinds of harmful substances should be included, and related specifications and issues should be further optimized, in order to better meet the requirement of occupational health care in the new era.

China ; Humans ; Occupational Exposure ; Occupational Health Services ; standards

Objective To compare the perioperative complications between Ivor-Lewis approach and McKeown approach in minimally invasive esophagectomy and gastric tube reconstruction for the treatment of middle and lower thoracic esophageal cancer. Methods Retrospective analysis of clinical data was performed on 288 patients with middle and lower thoracic esophageal cancer who underwent completely minimally invasive esophagectomy by one surgical team in Fujian Medical University Union Hospital from December 2010 to March 2014. Among the 288 patients , 103 patients underwent combined laparoscopic and thoracoscopic esophagectomy and intrathoracic esophagogastric anastomosis using a transoral anvil (Orvil) (Ivor-Lewis group, 2-incision) and 185 patients underwent combined laparoscopic and thoracoscopic esophagectomy and cervical anastomosis (McKeown group, 3-incision). Patients were stratified by surgical approach and perioperative outcomes were compared between the two groups. Results There were no statistical differences between two groups in intra-operative blood loss , conversion to open, extubation time, time to resume oral intake, postoperative hospital stay, the median number of lymph nodes resected. The operation time of Ivor-Lewis group was significantly shorter than that of McKeown group[(283.4±32.0) min vs. (303.6±43.7) min, P=0.003). The hospital cost of Ivor-Lewis group was significantly higher than that of McKeown group [(76 492 ±18 553) yuan vs. (68 923 ±17 331) yuan, P<0.01]. There were no statistical differences between two groups in chylothorax, delayed gastric emptying, atrial fibrillation, postoperative bleeding, admission to ICU, short-term postoperative mortality (P>0.05). The total postoperative complication morbidity of Ivor-Lewis group was significantly lower than that of McKeown group (16.5% vs. 31.4%, P<0.01). Ivor-Lewis group had lower pulmonary complication (8.7% vs. 25.9%, P<0.01), anastomotic leakage (1.9% vs. 13.0%, P<0.01), anastomotic stricture (0% vs. 4.9%, P<0.05), recurrent laryngeal nerve injury (1.0% vs. 7.0%, P<0.05). Conclusion Ivor-Lewis approach is associated with less postoperative complications, but higher cost as compared to McKeown approach in the treatment of middle and lower thoracic esophageal cancer.

Objective:To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia (CML) on the real-time quantitative PCR (RQ-PCR) detection of the BCR-ABL (P210) transcript levels.Methods:The peripheral blood samples of 84 CML patients were collected. The same sample was divided into different groups according to storage time (0, 6, 12, 24, 48, and 72 h) , temperature (room temperature, 18-24 ℃; low temperature, 2-8 ℃) , and vibration conditions (3, 6, and 12 h) . RQ-PCR was used to detect BCR-ABL (P210) transcript levels of the different groups. This study logarithmically transformed (log 10N) the original data [BCR-ABL copy number, ABL copy number, and BCR-ABL (P210) transcript levels]. Results:①Agarose gel electrophoresis showed significant RNA degradation of samples after storage for 48 and 72 h at room temperature. ②Among the overall samples, the BCR-ABL copy number of the samples stored at room temperature for 48 and 72 h was significantly lower than that of the samples stored at low temperature ( P<0.05) . However, the BCR-ABL (P210) transcript levels had no significant difference between samples stored at low temperature and room temperature. ③No significant changes were noted in the BCR-ABL (P210) transcript levels at different storage times (6, 12, 24, 48, and 72 h) regardless of storage temperature ( P>0.05) compared with that at baseline (0 h, -0.56±1.51) . ④ The BCR-ABL copy number of the overall sample only decreased significantly ( P<0.05) at 48 h (2.93±1.59) and 72 h (2.79±1.42) compared with that at baseline (0 h, 3.35±1.60) when stored at room temperature. The ABL copy number in the overall sample decreased significantly at 48 and 72 h (whether low and room temperature; P<0.05) . However, no significant changes were noted in the BCR-ABL (P210) transcript levels after vibration for 3 h (-1.29±1.81) , 6 h (-1.24±1.72) , and 12 h (-1.18±1.68; P>0.05) compared with that at baseline (0 h, -0.60±1.37) . Conclusion:Sample storage time, storage temperature, and vibration can interfere with the results of BCR-ABL and ABL copy number but have no significant effect on the quantitative determination of BCR-ABL (P210) transcript levels. This study provides strong support for the feasibility of transregional transportation of peripheral blood samples from patients with CML.

Objective To compare the perioperative complications between Ivor-Lewis approach and McKeown approach in minimally invasive esophagectomy and gastric tube reconstruction for the treatment of middle and lower thoracic esophageal cancer. Methods Retrospective analysis of clinical data was performed on 288 patients with middle and lower thoracic esophageal cancer who underwent completely minimally invasive esophagectomy by one surgical team in Fujian Medical University Union Hospital from December 2010 to March 2014. Among the 288 patients , 103 patients underwent combined laparoscopic and thoracoscopic esophagectomy and intrathoracic esophagogastric anastomosis using a transoral anvil (Orvil) (Ivor-Lewis group, 2-incision) and 185 patients underwent combined laparoscopic and thoracoscopic esophagectomy and cervical anastomosis (McKeown group, 3-incision). Patients were stratified by surgical approach and perioperative outcomes were compared between the two groups. Results There were no statistical differences between two groups in intra-operative blood loss , conversion to open, extubation time, time to resume oral intake, postoperative hospital stay, the median number of lymph nodes resected. The operation time of Ivor-Lewis group was significantly shorter than that of McKeown group[(283.4±32.0) min vs. (303.6±43.7) min, P=0.003). The hospital cost of Ivor-Lewis group was significantly higher than that of McKeown group [(76 492 ±18 553) yuan vs. (68 923 ±17 331) yuan, P<0.01]. There were no statistical differences between two groups in chylothorax, delayed gastric emptying, atrial fibrillation, postoperative bleeding, admission to ICU, short-term postoperative mortality (P>0.05). The total postoperative complication morbidity of Ivor-Lewis group was significantly lower than that of McKeown group (16.5% vs. 31.4%, P<0.01). Ivor-Lewis group had lower pulmonary complication (8.7% vs. 25.9%, P<0.01), anastomotic leakage (1.9% vs. 13.0%, P<0.01), anastomotic stricture (0% vs. 4.9%, P<0.05), recurrent laryngeal nerve injury (1.0% vs. 7.0%, P<0.05). Conclusion Ivor-Lewis approach is associated with less postoperative complications, but higher cost as compared to McKeown approach in the treatment of middle and lower thoracic esophageal cancer.