Cerebrolysin is an aqueous mixture of amino acids extracted from porcine brain.Cerebrolysin, contains a variety of amino acids and low molecular weight peptides, are permeable to the blood-brain barrier and blood-ocular barrier and can directly act on neurons in the CNS.As amino acid-based neuroprotective reagents, cerebrolysin can improve the neuronal metabolism, promote synapse formation and induce neuronal differentiation, and thus elicit neuroprotective efffectagainst insults such as ischemia , neurotoxins and so on.Cerebrolysin is clinicly used to treat eye diseases such as traumatic eye damage, glaucoma, optic atrophy ect.This paper systematically summarizes the studies on preclinical and clinical application of cerebrolysin in ophthalmology.

Objective:Discussion MKK4 protein expression in nasopharyngeal carcinoma and -1044 A/T polymorphism correlation.Methods:90 patients with nasopharyngeal carcinoma , MKK4 protein expression was determined by immunohistochemical staining,polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP ) to detect the gene -1044A/T sites monocytes nucleotide polymorphism.Results:MKK4 protein expression in nasopharyngeal carcinoma (-) was 24.4%(22/90),(+) was 15.6%(14/90),(++) was 34.4%(31/90),(+++) was 25.6% (23/90).Low expression (-/+) patients with a total of 36 cases,-1044AA genotype accounted for 22 cases (61.11%),AT genotype accounted for 12 cases (33.33%),TT genotype accounted for two cases (5.56%),AT+TT gene type accounted for 14 cases (38.89%).The patients with high MKK4 expression of 54 cases,of which accounted for 38 cases of AA genotype (70.37%),AT genotype accounted with 15 cases (27.78%),TT genotype accounted for one case (1.85%),AT +TT genotype accounted for 16 cases (29.63%).Low expression and high expression of T gene mutation occurs no significant ( Z=0.323 , P=0.747 ) .Conclusion: MKK4 protein expression correlated with -1044 A/T gene promoter polymorphisms was no significant correlation .

Cerebrolysin is an aqueous mixture of amino acids extracted from porcine brain, and mainly composed of 85% free amino acids and 15%small peptides. Cerebrolysin increase the metabolism of amino acids and glucose transport in the brain, improve the anti-anoxia ability of cells, and to enhance the brain's resistance to various types of malignant stimulation like stress and damage. Cerebrolysin can also promote synapse formation, induce neuronal differentiation, and help reverse brain injury. Because of its efficacy and safety, cerebrolysin has been widely used in the pediatric clinical practice, and primarily to treat neonatal hypoxic ischemic encephalopathy, childcerebral palsy, hyperactivity disorder, speech communication disorders, etc.The clinical symptoms were improved to some extent after the treatment of cerebrolysin. The recovery of consciousness, enhancement of comprehensionand memory, and improvement of the extremity motor function were observed. The treatment of cerebrolysincan not only enhance the cure rate, but also reduce the incidence of sequelae. This paper systematically summarized the clinical application of cerebrolysin in the pediatric population and relevant preclinical studies, to provide more guidance for clinical application of cerebrolysin in the treatment of pediatric diseases.

Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.

Objective To explore the effect of hydromorphone on hemodynamics after general anesthesia in elderly patients with hypertension under evaluation of Bioz noninvasive monitoring system.Methods Sixty elderly hypertension patients with selective abdominal operation under general anesthesia were randomly divided into hydromorphone group (HM group) and normal saline group (SP group),and 15 min before the end of surgery,hydromorphone was injected in the MH group,while the normal saline was injected in the control group.The indicators such as heart rate (HR),mean arterial pressure (MAP),cardiac output (CO),cardiac output index (CI) and systemic vascular resistance (SVR) were measured at different time points of before induction of anesthesia (T1),extubation (T2) and 10 min after extubation (T3).Recovery time of spontaneous breathing,awaking time,extubation time and out of PACU time were recorded.The visual analogue score (VAS),Ramsay sedation score at the PACU were also recorded at 5 min (H1),15 min (H2) and 30 min (H3).Results Compared with HM group,HR,MAP,SVR,VAS and Ramsay score increased significantly in the SP group (P ＜ 0.05).Compared with the indexes at T1,HR,MAP,SVR increased significantly while CO,CI decreased significantly at T2 (P ＜ 0.05).In both groups,SVR at T3 decreased but was significantly higher than that at T1 (P ＜ 0.0 5).compared between two groups at H1 time point in PACU,VAS and Ramsay score in group SP at H2,H3 time point were no different (P ＞ 0.05).VAS and Ramsay scores in HM group at H2,H3 were increased significantly (P ＜0.05).There were no significant differences in the recovery time of spontaneous breathing,awaking time and extubation time between two groups (P ＞ 0.05).Conclusion Bioz non-invasive hemodynamic monitoring system can provide effective hemodynamic information,and subcutaneous injection of 1 mg hydromorphone before the end of operation can effectively maintain the hemodynamic stability during the recovery period of general anesthesia in elderly patients with hypertension.

Objective To explore the effect of hydromorphone on hemodynamics after general anesthesia in elderly patients with hypertension under evaluation of Bioz noninvasive monitoring system.Methods Sixty elderly hypertension patients with selective abdominal operation under general anesthesia were randomly divided into hydromorphone group (HM group) and normal saline group (SP group),and 15 min before the end of surgery,hydromorphone was injected in the MH group,while the normal saline was injected in the control group.The indicators such as heart rate (HR),mean arterial pressure (MAP),cardiac output (CO),cardiac output index (CI) and systemic vascular resistance (SVR) were measured at different time points of before induction of anesthesia (T1),extubation (T2) and 10 min after extubation (T3).Recovery time of spontaneous breathing,awaking time,extubation time and out of PACU time were recorded.The visual analogue score (VAS),Ramsay sedation score at the PACU were also recorded at 5 min (H1),15 min (H2) and 30 min (H3).Results Compared with HM group,HR,MAP,SVR,VAS and Ramsay score increased significantly in the SP group (P ＜ 0.05).Compared with the indexes at T1,HR,MAP,SVR increased significantly while CO,CI decreased significantly at T2 (P ＜ 0.05).In both groups,SVR at T3 decreased but was significantly higher than that at T1 (P ＜ 0.0 5).compared between two groups at H1 time point in PACU,VAS and Ramsay score in group SP at H2,H3 time point were no different (P ＞ 0.05).VAS and Ramsay scores in HM group at H2,H3 were increased significantly (P ＜0.05).There were no significant differences in the recovery time of spontaneous breathing,awaking time and extubation time between two groups (P ＞ 0.05).Conclusion Bioz non-invasive hemodynamic monitoring system can provide effective hemodynamic information,and subcutaneous injection of 1 mg hydromorphone before the end of operation can effectively maintain the hemodynamic stability during the recovery period of general anesthesia in elderly patients with hypertension.

OBJECTIVE To explore the anti-respiratory syncytial virus(RSV) pharmacodynamic material basis of Scutellaria Baicalensis. METHODS Network pharmacology method was used to analyze the anti-RSV targets of Scutellaria baicalensis. UPLC-QTOF-MS/MS was used to characterize the consensus components in Scutellaria Baicalensis. A mouse model of RSV pneumonia was established, and the changes of mouse body weight, lung index, lung pathological sections and IL-6 were detected. The gray correlation method was used to analyze the spectrum-effect data of 50 batches of Scutellaria baicalensis samples, and the effective components of Scutellaria baicalensis against RSV pneumonia mice were mined. RESULTS The protein-protein interaction network results determined that the core targets of Scutellaria baicalensis against RSV were AKT1, IL-6, TNF, MAPK3, SRC, HSP90AA1 and PTGS2; overall animal experiment proved that lung index of Scutellaria baicalensis decreased to varying degrees, and the inflammatory factor IL-6 had significant differences; grey correlation analysis showed that the anti-RSV chemical components in Scutellaria baicalensis were mainly flavonoid glycosides.CONCLUSION Using the network pharmacology method to determine the pharmacodynamic target, the gray correlation degree analysis component-target data, the method of mining the pharmacodynamic material basis is feasible, baicalin, wogonin, chrysin-6-C-arabinose-8-C-flavonoid glycoside, chrysin-6-C-arabinose-8-C-arabinoside and chrysin-7-O-glucuronide can be used as Q-Markers of Scutellaria baicalensis for quality evaluation.