Objective: To develop a polyvinylidene fluoride (PVDF)-based instrument which may be used to measure bite force in natural teeth as well as in denture. Methods: The PVDF film was used as transducer. Electricity amplifier, lowpass filter, secondary magnifier in metrical circuit, and collection with disposal system were employed to produce the instrument for bite force measurement. The demarcating system was MTS-858. The instrument was used to measure the dynamic force in laterotrusion in 9 volunteers with Angle I occlusion. Results: The instrument was produced.By the used of the instrument the peak value of bite force (N) in the 9 subjects was 277.3?78.4 on the main functional side (MFS) and 258.7?83.3 on the subfunctional side (SFS).The time(ms) needed in the four phases on MFS was 65.6?7.3,354.4?66.1,116.3?25.0 and 226.9?35.8;that on SFS 67.5?6.6,394.4?65.3,141.9?25.6 and 167.5?41.9,respectively.Conclusion: The new system is steady and sensitive for bite force measurement.

Objective To explore the relationship between primary hypothyroidism and hyperuricemia and to speculate their possible mechanism .Methods The levels of FT3 ,FT4 ,TSH, Anti-TG ,Anti-TPO, TRAb, UA, TC, TG, LDL-C, HDL-C,Hcy, Cys,β2 MG and 24hUA were measured in 36 patients with hypothyroidism , compared with 20 healthy sub-jects.According to the absence or presenceof Anti-TG, Anti-TPO and TRAb, the patients were divided into two sub-groups:the Hashimo to(HT) hypothyroidism group (27 cases) and the non-HT hypothyroidism group (9 cases)before they were compared with each other .Results Compared with healthy subjects , patients with hypothyroidism had higher TSH , TC,TG, LDL-C, UA, Hcy,Cys,andβ2MG,and lower FT3,FT4,HDL-C,and 24hUA.The difference was significant (P<0.05);the thyroid function, autoimmune antibodies, TC, LDL-C, UA and 24hUA were also significantly differen between the two sub-groups.Conclusion Patients with hypothyroidism are more prone to hyperuricemia , which may be related to decreased thyroid hormone , lipid metabolism disorder and hyperhomocysteine .The level of UA is higher in HT hypothyroidism than in non-HT hypothyroidism , indicating that autoimmune factors may be involved in the occurrence of hyperuricemia .

Objective To investigate the effects of normal caloric high-protein diet on metabolic parameters and gastrointestinal hormones in patients with obesity or type 2 diabetes mellitus (T2DM).Methods Totally 30 obese subjects (OB group) and 40 obese patients with type 2 diabetes mellitus (T2DM group) received normal caloric high-protein diet for 24 weeks.Then body weight,fat mass,and metabolic parameters were assessed.The serum ghrelin and glucagon-like peptide-1 (GLP-1) levels were also measured at 0 and 2 h after an oral 100 g of steamed bread meal test.Results After 24 weeks,body weight [(78.23 ± 7.51) kg vs (80.83±8.13) kg,P=0.016; (80.45±7.48) kg vs (83.26±9.35) kg,P=0.011],body mass index [(31.03 ±2.72) kg/m2 vs (32.01 ±2.95) kg/m2,P=0.033; (32.63 ±2.81) kg/m2 vs (33.86±3.03) kg/m2,P =0.043],waist circumference [(93.65 ± 6.23) cm vs (97.30 ± 7.81) cm,P =0.041 ;(97.02±7.43) cm vs (101.87 ±9.87) cm,P =0.034],fat mass [(30.42 ±6.18) kg vs (32.47 ±5.91) kg,P=0.022; (34.23 ±7.03) kg vs (36.64 ±6.83) kg,P =0.032],fasting serum insulin [(10.81±3.69) mmol/Lvs (13.58±4.86) mmol/L,P=0.012; (9.58±3.51) mmol/Lvs (10.82±4.28) mmol/L,P =0.015],HOMA-insulin resistance index [(2.42 ± 0.83) vs (3.16 ± 1.21),P =0.019; (3.15±0.74) vs (4.13±0.67),P=0.024] and triglycerides [(1.24±0.32) mmol/Lvs (1.49±0.52) mmol/L,P=0.046; (1.86±1.05) mmol/L vs (2.46±1.85) mmol/L,P=0.034] were significantly decreased in OB and T2DM groups,respectively,compared with 0 week.Fasting blood glucose (FBG)and glycosylated hemoglobin were also significantly declined in T2DM group [FBG:(6.73 ± 1.25) mmol/Lv s (8.63 ±2.81)) mmol/L,P=0.010; HbA1c (6.44 ±0.47) vs (7.38 ±0.33),P=0.031].The serum ghrelin and GLP-1 both increased at 0h and 2 h after meal test in OB and T2DM groups [fasting serum ghrelin:(4.98 ±0.89) μg/L vs (3.95 ±0.98) μg/L,P=0.021; (4.23 ± 1.67) μg/L vs (3.15 ± 1.01) μgL,P=0.025; 2 h postprandial serum ghrelin:(2.98 ±0.96) μg/L vs (2.56 ±0.83) μg/L,P =0.046; (2.83 ± 1.03) μg/L vs (1.95 ±0.92) μg/L,P =0.033; fasting serum GLP-1:(6.06 ±0.63) μg/L vs (5.13±0.59) μg/L,P=0.041; (5.23±0.71) μg/Lv s (4.49±0.53) μg/L,P=0.039; 2 h postprandialserum GLP-1:(10.01±1.01) μg/L vs (7.68±0.94) μg/L,P=0.007; (8.87±0.94) μg/Lvs (6.59 ± 0.87) μg/L,P =0.013].Conclusions Normal caloric high-protein diet can reduce body weight and serum glucose.Those effects may be due to the increase in secretion of GLP-1.

ObjectiveTo evaluate the effect and complication of whole γ-knife stereotactic radiotherapy on the non-small cell lung cancer and explore the value of γ-knife stereotactic radiotherapy in treatment of non - small cell lung cancer. Methods42 patients with non -small cell lung cancer:γ-knife radiotherapy patients ,4 ～ 8Gy once a day,five times a week,4 ～ 10times and 40 ～50Gy in total. ResultsThe overall response rate (CR + PR) in γ-knife was 71.4％. The1-,2-and 3-year survival rates were 95.5％, 59.5％, 33.3％. ConclusionCompared with conventional radiation,γ-knife stereotactic radiotherapy could achieve tumor dose escalation,and give the normal tissue better protection at the same time. It could improve the tumor control rate of non-small cell lung cancer,improve long survival rate,and so could reduce the radiation reaction.

Seventy newly diagnosed diabetic patients were enrolled in the study, including 37patients aged over 60 (elderly-onset diabetes, EOD group) and 33 patients under 60 (adult-onset diabetes,AOD group) ; 30 elderly healthy subjects served as controls. Serum melatonin levels were determined with ELISA and the expression of inducible nitric oxide synthase (iNOS) mRNA in peripheral blood leucocytes (PBL) was detected by reverse transcription polymerase chain reaction (RT-PCR). The results showed that serum melatonin levels were significantly lower in EOD group than those in control group [ (6. 6 ± 1.3 )ng/L vs. (7.7 ± 1.4) ng/L, P ＜0. 01 ], the expression of iNOS mRNA was significantly higher than that in AOD group [ (36 ± 3 )% vs. (30 ± 4)%, P ＜ 0. 01 ]; and i NOS mRNA was not detected in control group.Logistic regression analysis indicated that decreased serum melatonin levels and up-regulated iNOS mRNA might be independent risk factors for diabetes mellitus in the elderly.

BACKGROUND:Drug therapy can partly reduce and delay the progress of Alzheimer’s disease, but only 30%with the single drug treatment obtain clinical cure. OBJECTIVE:To study the therapeutic effect of bone marrow mesenchymal stem cel s transplantation for rats with Alzheimer’s disease. METHODS:Amyloidβ-protein was injected into the hippocampus of Sprague-Dawley rats to construct the model of Alzheimer’s disease. And bone marrow stromal stem cel s were transplanted into the hippocampus of the rat models. RESULTS AND CONCLUSION:At 2 weeks after modeling, compared with the control group, the escape latency in the model and experimental groups was significantly longer (P<0.05), which indicating that Alzheimer’s disease models were successful y established. At 4 weeks after cel transplantation, compared with the model group, the average escape latency in the experimental group was significantly decreased, but retention time on the platform quadrant was significantly prolonged (P<0.05). Besides, at 4 weeks after cel transplantation, expression of choline acetyltransferase in the experimental group was significantly higher than that in the model group (P<0.05). In conclusion, bone marrow mesenchymal stem cel s cannot only differentiate and survive in the hippocampus of rats with Alzheimer’s disease, but also improve the learning and memory ability.

BACKGROUND:Drug treatment for senile dementia has unsatisfactory outcomes although to a certain extent it can reduce and delay the progression of Alzheimer’s disease. Stem cel transplantation is a new attempt for the treatment of senile dementia.
OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cel transplantation on the behavior of senile dementia rats.
METHODS: Rat models of senile dementia were made in 20 Sprague-Dawley rats that were given continuous 60-day gavage of aluminium chloride solution. Then, model rats were randomized into model group treated with normal saline injection and experimental group treated with hippocampal injection of bone marrow mesenchymal stem cels, respectively. Another 10 rats undergoing normal feeding served as control group. Learning and memory ability of rats were tested by Morris water maze, and superoxide dismutase activity and malondialdehyde content in brain tissues of rats were measured by colorimetric method at 4 weeks after cel transplantation.
RESULTS AND CONCLUSION:Compared with the model group, the escape latency was shortened and the cross-platform frequency was increased in the experimental group (P < 0.05), and moreover, significantly elevated superoxide dismutase activity and reduced malondialdehyde content in the brain tissues of rats were found in the experimental group (P < 0.05). These findings indicate that bone marrow mesenchymal stem cel transplantation contributes to behavior improvement in senile dementia rats by improving the learning and memory ability.

Objective:To screen the binding peptide against adenovirus type 7(Ad7) and evaluate the relevance with the ade-novirus receptor .Methods:Binding peptide against Ad 7 was screened by panning the phage display 12 peptides library .The antibody against the selected peptide was prepared and was used to study the binding to the membrane by immunofluorescence technique .Re-sults:Using Ad7 as the target protein , GTS09 peptide was selected from the phage display 12 peptides library by biopanning .GTS09-phage complex could significantly bind Ad 7, with the affinity constant up to 1.93 ×1010 L/mol;at the same time, immunofluorescence showed that antibody of GTS09 could specifically bind to membrane of 293 cell.Conclusion: Antibody against GTS09 peptide could specifically bind to membrane of 293 cell,which shows that the peptide may presumably have homology with the cell receptors of Ad 7.

Objective To investigate the effect of fosinopril on the expression of transforming growth factor (TGF)-activated kinase 1 (TAK-1) in the kidney of rats with unilateral ureteral obstruction (UUO),and its protective effect on renal interstitial fibrosis (RIF).Methods UUO model was induced by ligating the left ureter in rats.A total of 72 male Sprague-Wistar rats were randomly divided into 3 groups:sham-operated group (n=24),UUO model group (n=24) and UUO+fosinopril group (n=24).The rats were treated with 10 mg/(kg?d) by gastric gavage in the fosinopril treated group from 2 d after the operation,and the rats were treated with the identical dose of normal saline in the other 2 groups.Eight rats of each group were sacrificed at 7,14 and 21 d after UUO.Pathological changes of the renal tissue were observed by HE and Masson staining,and the mRNA and protein expression of TAK1 was detected by in situ hybridization,real-time PCR and Western blot analysis.Results The renal interstitial damage index of UUO group was increased compared with that of the sham-operation group (P

Objective To investigate the impact of renal function and insulin resistance on serum total homocysteine(tHcy) levels in aged diabetic patients with nephropathy. Methods Serum tHcy, blood glucose, lipids, renal function, serum insulin were measured for 98 elderly patients with type 2 diabetes and 36 normal control subjects. Results (1) Serum tHcy levels were significantly higher in diabetic group than in control group [(17.2?7.6) ?mol/L vs (9.4?3.5)?mol/L,P