Objectives To study the clinical significance of the early diagnosis on colorectal cancer and the value of monitoring precancerous lesions of p53 gene examination. Methods Histological specimens of tumor from 110 patients with colorectal neoplasms were collected under the endoscopy, another 10 normal intestinal mucosa tissues with benign enteropathy as a control group. By PCR-SSCP methods, examined p53 exon 5～8 of mutation circumstance, made pathological section at the same time, and combine to track the observation. Results 24 patients p53 gene mutation were detected out in 78 cases colorectal cancers,counted up 32 4%, among 17 patients were moderate,low differentiation, 3 patients of them were mucinous adenocarcinoma.There are 6 patients p53 gene mutation in 36 cases colorectal adenoma,counted up 16 7%, among them an patient of Ⅱ～Ⅲ class atypical hyperplasia adenoma occurrence canceration in the next 10 months.There was no p53 gene mutation in normal intestinal mucosa tissue.Conclusions p53 gene examination combinated with pathological examination could monitor precancerous lesions and early diagnosis of the colorectal cancer.

BACKGROUND:Col agen-gelatin composite scaffolds have been reported to be able to promote the early recovery of peripheral nerve defects. However, this conclusion has not been further confirmed. OBJECTIVE:To investigate the biocompatibility of the col agen-gelatin scaffold and its treatment outcomes in the repair of peripheral nerve defects. METHODS:The col agen-gelatin scaffold was co-cultured with bone marrow mesenchymal stem cel s (BMSCs) of Sprague-Dawley rats for 5 days, and then the cel growth was observed. Twenty Sprague-Dawley rats were enrol ed, modeled into a left 30-mm peroneal nerve defect and randomized into experimental and control groups. The col agen-gelatin scaffold composited with BMSCs was implanted into the experimental group, and autograft nerve implanted into the control group. Morphology of the middle bridge was observed, and electrophysiology was conducted at 16 weeks after implantation. RESULTS AND CONCLUSION:BMSCs grew and adhered wel onto the scaffold. The myelinated nerve fiber density did not significantly differ between groups (P>0.05). The myelinated nerve fiber diameter, myelin sheaththickness and percentage of nerve tissues in the experimental group were significantly lower than those in the control group (P<0.05). There were no significant differences in the conduction velocity, latency of motor nerves and the conduction velocity and amplitude of sensory nerves between groups (P>0.05). The amplitude of motor nerves and the latency of sensory nerves in the experimental group were significantly lower than those in the control group (P<0.05). To conclude, the col agen-gelatin scaffold holds a good cytocompatibity and is ideal for the repair of peripheral nerve defects.

Objective To investigate the clinical significance of observing the dynamic morphological changes of circle cistern on CT scanning in the patients with acute severe traumatic brain injtuy (TBI).Methods The CT imaging data of circle cistern of 135 patients with acute severe TBI were analyzed retrospectively.According to the morphological changes of circle cisterns on the initial CT images,135 cases were divided into 5 groups,and 135 cases carried on prognostic evaluation with Glasgow outcome score (GOS) 6 months later.The relationship between the dynamic morphological changes of circle cistern and the prognosis of patients were analyzed statistically.Results There was a significant relationship between the state of circle cistern and the prognosis of patients.The more obvious the dynamic morphological changes of circle cistern were,the worse prognosis and the higher the fatality was,especially higher mortality in patients with exacerbation of the dynamic morphological changes of circle cistern (79.5％),and the difference was statistically significant among 5 groups (P ＜ 0.01).Conclusions There was a significant relationship between the state of circle cistern and the prognosis of the patients with acute severe TBI.The dynamic morphological changes of circle cistern on CT scanning might be used as a promising marker to evaluate the severity of severe TBI,especially in the patients at earlier posttraumatic interval.It is necessary to observe the dynamic change of circle cistern.

Objective To investigate the clinical effects of subeutanous fistula excision for the long-path low anus fistula.Methods Operation for long-path low anus fistula,fistula length＞5cm,divided the pafients into two groups.The therapeutic group:using subcutanous fistula excision;the control group:using fistula icision.Comparing the healing time,arIus malformation,recurrence rate.Results Therapeutic group 47 cases heal,the healing time was 12 days;no anus malformation;no arms ineontenience.In the control group,the healing time was 23 days;anus malformation 23 cases;anus gas incontenience 12 cases.Conclusion To use subcutanous fistula excision for the longpath low anus fistula has the advantages of short healing time,no anus malformation,less pain,and low reccurrent rate.

Objective:To explore the role and significance of IL-17 on the pathogenesis of rheumatoid arthritis ( RA).Methods:The levels of IL-17 mRNA in peripheral blood mononuclear cell ( PBMC) of 66 RA patients (32 active,34 inactive) and 44 healthy controls were detected by reverse transcription-polymerase chain reaction ( RT-PCR ).The levels of serum IL-17 were detected by enzyme-linked immunosorbent assay ( ELISA).The levels of RF were detected by immunoturbidimetric assay.The correlations of serum IL-17 and RF were analyzed.Results: In RA patients the levels of IL-17 mRNA in PBMC were significantly higher than those in healthy controls ( P＜0.05 ) , but there was no difference between the active and inactive RA ( P>0.05 ).The serum IL-17 levels in active RA patients were significantly higher than those in inactive RA patients and healthy controls ( P＜0.05 ).The levels of serum IL-17 were positively correlated with RF in RA patients.Conclusion: IL-17 and RF play an important role in the pathogenesis and pathology process of RA ,and IL-17 may be associated with RA activity.

[ ABSTRACT] AIM:To investigate the inhibitory effect of Am80 on neointima hyperplasia in carotid arteries after balloon injury and to observe the interaction between Krüppel-like factor 4 (KLF4) and retinoic acid receptorα(RARα). METHODS:Neointima hyperplasia in carotid arteries was observed by hemotoxylin and eosin staining.The expression of KLF4 and cyclin D1 was examined by immunostaining and Western blotting analysis.To detect the interaction between KLF4 and RARαin the vascular tissue, the injured arteries were harvested, and the protein extracts were prepared and subjected to co-immunoprecipitation assay.RESULTS:Compared with injured group, Am80 significantly reduced neointi-mal hyperplasia and the thickness ratio of intima to media.Am80 not only up-regulated KLF4 or RARαexpression in caro-tid arteries, but also increased the interaction between KLF4 and RARαat tissue levels.CONCLUSION:Am80 inhibits neointima hyperplasia in carotid arteries after balloon injury by promoting the interaction between KLF4 and RARα.

Objective:To study the effects of NS-398 on the expression of vascular endothelial growth factor (VEGF) in human tongue squamous cell carcinoma Tca8113 cells.Methods:Tca 8113 cells were exposed to the COX-2 inhibitor NS-398 at 150 ?mol/L for 48,72 and 96 h respectively,then cells were collected. VEGF expression was examined by RT-PCR and Western blot. Results:NS-398 inhibited VEGF mRNA and protein expression time-dependently in Tca8113 cells. Conclusion:NS-398 may inhibit VEGF expression in tongue squamous cell carcinoma.

Objective To evaluate the effectiveness and safety of a transanal drainage tube(TDT) for decompression of acute left-sided obstruction of colorectum.Methods Fifty-seven patients with acute left-sided colorectal obstruction were enrolled in this study from January 2010 to December 2014.The obstruction location, property, success rate of insertion, one-procedure rate and complication rate were analyzed.Results There were 53 cases of primary colorectal cancer,among which lesions were located in the transverse colon in 1 case, in descending colon in 10, in sigmoid colon in 24,and in rectum in 18.There were 4other cases, one sigmoid colonic metastases of pulmonary cancer, 1 adhesive colonic obstruction after ovary surgery, 1 cervical cancer involved with rectum with stricture and obstruction, and 1 descending colonic obstruction caused by Crohn's Disease.TDT was successfully inserted in 55 cases(96.5％) without complications,in which 43 cases of primary colorectal cancer finally underwent surgery.TDT indwelled from 0 to 22days, for an average of (8.7± 4.4)d.Hartmann operation was performed in 9 patients,6 of which underwent sufficient lymphnode dissection.Among the 43 patients, one-stage operation was performed in 34 (79.1％),of which 30 cases underwent sufficient lymph node dissection, without stoma leakage.And the rest of 13 cases refused surgery because of poor prognosis and financial problems.One patient with Crohn's Disease refused surgery after TDT insertion and was discharged after palliation of distention.Conclusion TDT is safe and effective in decompressing acute left-sided benign obstruction with high success rate and low expenditure, and can achieve preoperative colonic lavage in one-stage operation for acute left-sided colorectal malignant obstruction.

Objective To investigate and summarize the clinical characteristic of autoimmune pancreati-tis(AIP).Methods Clinical data of 13 patients with AIP, admitted to the West China Hospital from 2009 to 2013, were retrospectively analyzed .Results The median age of the 13 patients was 48 years old.The main clinical manifestations included epigastric pain or discomfort (8 cases), obstructive jaundice(7 cases)and weight loss(10 cases).Diffuse enlargement of pancreas and localized pancreatic enlargement were observed in 6 cases, pseudocyst and pancreatic stones were observed in 1 case.Dilation of pancreatic duct was found in 4 cases.Bili-ary stricture and thickened wall of bile duct were detected in 8 cases, dilated gallbladder with delayed enhance-ment of the thickened wall in 7 cases, retroperitoneal fibrosis surrounding the aorta in 1 case, abdominal lymph-adenopathy in 6 cases, stenosis of splenic vein in 4 cases, and Sjogren's disease and ulcerative colitis in 1 case. The positive rate of serum IgG, RF, ANA was 71.43%(5/7), 42.86%(3/7), and 42.86%(3/7), respec-tively.8 patients were misdiagnosed and underwent surgery .Steroid therapy was administered in all patients with satisfactory response.Conclusions AIP is a rare and autoimmune disease ,without speci? c symptoms,and often be misdiagnosed as pancreatic cancer or bile duct carcinoma .We must give more attention to AIP and keep pa-tients from undergoing unnecessary surgery .

OBJECTIVE:To investigate whether the magnesium sulfate injection(MSI)into cisterna magna in a rabbit with subarachnoid hemorrhage(SAH)can reverse the cerebral vasospasm and damage of brain tissues.METHODS:The single-hemorrhage SAH rabbit model was used.Thirty New Zealand white rabbits were randomly divided into the following three groups(n=10,in the each group):sham group,model group and MSI group.At 24 hours after SAH,the rabbits were injected with 0.1 mL?kg-1 of saline into cisterna magna in sham and model groups,while 0.1 mL?kg-1 of 4% magnesium sulfate was injected into cisterna magna of rabbits in MSI group.All animals were sacrificed at 48 hours after SAH.Basilar arteries and hippocampus were then removed for measurement of the cross-sectional areas of basilar arteries and the hippocampus normal neuron density of CA1 regions.RESULTS:The cross-sectional areas of basilar artery and the hippocampus normal neuron density of CA1 regions were smaller in model group than in the other two groups(P0.05).CONCLUSIONS:This study results indicate that injecting magnesium sulfate into cisterna magna can reverse the cerebral vasospasm and the following hippocampal neuron damage in rabbits with subarachnoid hemorrhage.