Objective To explore the relationship among personality,job burnout and knowledge sharing behavior of the enterprise employees.Methods The 44 items big five inventory(BFI) ,knowledge sharing inventory and job burnout inventory were administrated to 635 employees online.Results ( 1 ) Agreeableness had a significant main effect on knowledge sharing ( β =0.135, P＜0.01); (2)Cynicism( β = -0.140, P＜0.01) and reduced personal accomplishment( β = -0.125, P＜0.01 ) had a significant effect on knowledge sharing behavior and its two dimensions; (3) Job burnout partially mediated the relationship between agreeableness and knowledge sharing behavior(P＜0.01).Conclusion Enterprise employees' personality had different effects on knowledge sharing behavior,and job burnout was an important mediating role in the relationship above.

Objective To observe the THP for locally advanced breast cancer in recent efficacy and toxicity. Methods 32 patients with locally advanced breast cancer received chemotherapy: THP 50mg/m~2 , intravenous injection; day 1: CTX600mg/m 5 - FU500mg/m~2, ivgtt on day 1 and day 8,for a period of 21 days. Results 73% had efficiency,with small heart poisonous side effects. Conclusion New adjuvant chemotherapy with THP - containing regimen for the treatment of locally advanced breast cancer has a higher efficacy, and adverse reactions can be tolerated.

Objective To study the effect of knockdown A20 expression on the proliferation, apoptosis and migra-tion of MCF-7 cells and to evaluate the potential value of the A20 gene as the therapeutic target of breast cancer. Methods Synthesized siRNA targeted to A20 gene or negative control siRNA were transfected into MCF-7 cells by using lipofectamine 2000. CCK8 assay, Annexin V and 7-AAD double staining cytometry, Transwell assay were performed to investigate the effect of knockdown A20 mRNA expression on the proliferation, apoptosis, migration of MCF-7 cells, respectively. Results It can inhibit the proliferation and migration as well as promote the apoptosis in MCF-7 cells by knockdown A20 mRNA expression. Conclusion A20 gene plays an important role in the prolif-eration, apoptosis and migration of MCF-7 cells and it could be a potential therapeutic target of breast cancer.

Objective To compare the count of circulating endothelial cells (CECs) between patients with reversible pulmonary hypertension (RPH) and irreversible pulmonary hypertension (iRPH) by flow cytometry in order to find a new biomarker to distinguish RPH from iRPH.Methods Ninety pulmonary hypertension associated with congenital heart disease patients treated in our hospital between September 1,2013 to March 31,2014 were enrolled in this study.According to the mean pulmonary arterial pressure (mPAP) measured by echocardiography in six months after treatment,the patients were separated into 2 groups:iRPH group(mPAP≥50 mmHg),RPH group(mPAP ＜50 mmHg).Results Totally the clinical data of 87 patients were enrolled in the statistic analysis,86 patients finished the follow-up in 6 months after the surgery,1 patient died from pulmonary hypertensive crisis.The count of CECs was not correlated with the extent of pulmonary hypertension(P =0.925).The number of CECs was higher in iRPH group than that of RPH group(0.46‰ vs.0.09‰,Z =-5.021,P =0.000).And also the age of patients in iRPH group was elder than that of patients in RPH group [(43.1 ± 37.4) months vs.(9.3 ± 12.6) months,t =-5.079,P =0.000].Conclusion The count of CECs is significantly increased in iRPH patients.It could be used as one biomarker to distinguish RPH from iRPH.

Objective To explore the relationship among absolute income,big five personality and life satisfaction,and the mediating effect of relative income.Methods 5927 citizens from different cities were assessed by NEO five-factor inventory (NEO-FFI) and satisfaction with life scale (SWLS).Results (1) The mean score of life satisfaction was 19.61 ± 5.99.Female scored higher than male,and there were statistically significant differences between them (19.98 ± 5.92 vs 19.14 ± 5.95,t =-5.08,P ＜ 0.01).(2) The absolute income,extraversion and conscientiousness had a significant main effect on life satisfaction (β =0.13 ～ 0.19,P ＜ 0.01),neuroticism,openness and agreeableness had a significant main negative effect on life satisfaction (β =-0.04--0.15,P＜0.01).(3)The relative income had a significant main effect on life satisfaction (β =0.35,P ＜0.01).(4)Relative income had a mediating effect on the relation between absolute income,neuroticism,extraversion,agreeableness,conscientiousness and life satisfaction (P ＜ 0.01).Conclusion Relative income has a mediating effect on the relation between absolute income,personality and life satisfaction.

Objective To strengthen the understanding of the imaging features in congenital aberrant left pulmonary artery (CALPA). Methods All 4 patients underwent chest film and Doppler echocardiography. Thres cases were examined by enhanced spiral CT examination. In addition, MRI and DSA were performed in 2 cases respectively. Thres cases were proven by surgery or autopsy. Results (1) appearances on chest films included cardiomegaly, pulmonary overvascularity, left hilum a little lower than the right one, emphysema, atelectasis, and pneumonia. (2) bronchography showed stenosis of trachea and/or bronchia. (3) Barium esophagram showed an imprint on the left anterior wall of esophagus. (4) Contrast-enhanced spiral CT and MRI showed marked dilatation of main pulmonary artery (MPA) and the root of MPA extended backward to become right pulmonary artery (RPA), the left pulmonary artery (LPA) arose from the RPA directly. (5) echocardiography indicated LPA arose from RPA. (6) DSA showed MPA was marked dilated, and LPA arose from RPA. (7) other abnormalities included 3 PDA, 2 PLSVC, and ~1 ASD. Conclusion CT and MRI are the best methods to diagnose CALPA.

Objective To investigate the distribution and antibiotic resistance of gram-negative bacilli for better antimicrobial therapy in our hospital.Methods A retrospective analysis was conducted for the 1 060 strains of gram-negative bacilli isolated from clinical specimens during 2013.Results Of the 1 060 gram-negative bacterial strains isolated during 2013,E.coli,K . pneumoniae,P .aeruginosa and A.baumannii were the leading pathogens,accounting for 29.3%,22.8%,11.5% and 9.9%,respectively.The prevalence of extended spectrum-lactamases (ESBLs)positive strains was 63.7%,32.2% and 28.0% in E.coli,K .pneumoniae and P .mirabilis,respectively.The Enterobacteriaceae strains were highly sensitive to carbapenems.The percentage of the P .aeruginosa isolates resistant to meropenem,imipenem or amikacin was lower than 30%.The percentage of the Acinetobacter spp.(A.baumannii accounted for 70.9%)strains resistant to meropenem and imipenem were 25.0% and 26.2%.Conclusions Most of the gram-negative bacilli are resistant to multiple antimicrobial agents. We should strengthen the monitoring of the antibiotic resistance of gram-negative bacilli and optimize antimicrobial therapy.

Objective To study the removal effect of Moringa seedprotein on turbidity water. Methods The protein of Moringaoleifera seed was extracted by salting out and salting out methodand the protein concentration of Moringa oleifera seed wasdetermined by Coomassie blue staining;The removal effect of Moringa seed protein on turbidity water was determined by coagulation test.The contents of COD, ammonia nitrogen and nitrate nitrogen in the water were determined to determine the effect of Moringa seed protein on water quality. Results The experimental results showed that Moringa oleifera seed protein has good removal effect on high and medium turbidity water, and its removal effect is in a dose - dependent manner.The removal rate of 7 mg/L of Moringa seed protein to high and medium turbidity water reached 92.25 ％ and 64.71 ％ respectively. But the removal efficiency of low turbidity water was less than 7 mg/L, the removal rate of low turbidity water was only 31.91％.The results of determination of COD, ammonia nitrogen and nitrate nitrogen showed that the Moringa seed protein did not increase the content of organic matter in the water while removing turbidity effectively. Conclusion Moringa oleifera seed protein has a certain removal effect on turbidity water, among which the removal effect of high and medium turbidity water is strong, and the removal effect of low turbidity water is poor.Moringa oleifera seed protein had little effect on water quality.

Objective To investigate the distribution and antimicrobial resistance of the clinical isolates from Children's Hospital of Chuzhou during 2014.Methods A retrospective analysis was conducted with the bacterial strains isolated from various clinical specimens in 2014.Results A total of 382 clinical isolates were collected during 2014, of which gram positive organisms and gram negative organisms accounted for 37.4 % and 62.6 %, respectively. The top 5 most frequently isolated microorganisms were E. coli (18.8 %),K. pneumoniae (16.8 %), coagulase negative Staphylococcus (13.1 %),S. pneumoniae (9.4 %) and S. aureus (9.2 %). The prevalence of MRSA was 28.6 % in S. aureus and the prevalence of MRCNS was 76 % in CNS. All staphylococcal strains were susceptible to daptomycin, linezolid and vancomycin. All the E. faecalis and E. faecium isolates were sensitive to daptomycin, linezolid and vancomycin. All the S. pneumoniae strains were susceptible to penicillin. The prevalence of extended spectrum-lactamases (ESBLs) positive strains was 58.3 % in E. coli and 28.1 % in K. pneumoniae. The Enterobacteriaceae strains were highly sensitive to carbapenems. Only 3 (1.6 %) carbapenem-resistant strains were identified in the Enterobacteriaceae isolates. About 13.3 % and 6.7 % of theP. aeruginosa isolates were resistant to piperacillin and levofloxacin, respectively. All the P. aeruginosa strains were sensitive to the other antimicrobial agents. The percentage of carbapenem-resistantAcinetobacter strains was lower than 10 %. Only one carbapenem-resistant A. baumannii strain was identified.Conclusions Gram negative microorganisms account for most part of the clinical bacterial isolates in 2014. The antimicrobial resistance is still very serious in this hospital, especially the emergence of carbapenem-resistant Enterobacteriaceae, which is of great concern.

Objective To evaluate the risk factors,diagnostic index of the restrictive right ventricle physiology(rRV) and the impact of the mid-term outcome of the patients.Methods Eighty patients(30 in rRV group and 50 in non-rRV group) undergoing TOF repair admitted in our department from Oct 2011 to May 2012 were studied.Perioperative clinical data were collected and echo data were recorded after operation.Mixed linear regression for repeated measures was used to compare the variables and analyze the correlations.Results Patients in rRV group were younger with longer cardiopulmonary bypass(CPB) time,aortic cross clamp time,ventilation time,intensive care unit and hospital stay compared with those in non-rRV group(P<0.01,respectively).The younger patients with longer CPB time had high risk of rRV by logistic regression analysis.Within 7 days post operation,the increase of saturation of venous oxygenation and decrease of oxygen extraction ration were slower in rRV group than those in non-rRV group(P<0.05,respectively).Lactate decreased in both groups,but was higher in rRV group throughout the 7 days(P=0.03).NT-proBNP was higher in rRV group throughout the 7 days than that in non-rRV group.NT-proBNP≥4750pg/ml often indicated the patients in the state of rRV.CRP slightly increased in 1-2 days post operation,and decreased thereafter,and the decrease was slower in rRV group(P=0.08).With regard to the mid-term outcome,there was no significant differences in the incidences of the obstruction of the right ventricle outflow and main pulmonary artery,the stenosis of the branch of pulmonary artery and the degree of the pulmonary valve regurgitation.Conclusion rRV is associated with significantly higher levels of NT-proBNP and CRP.The incidence of rRV correlates with age on operation and positively correlates with CPB time.NT-proBNP would be regarded as an indicator of the incidence of rRV.The study indicated the rRV would have impact on the early outcome of the patients but there was no significant effect on mid-term outcome.