BACKGROUND:Several studies have demonstrated that many kinds of Chinese herbs for benefiting vital energy and enriching the blood secrete some cytokines by influencing bone marrow stromal cel s to promote the differentiation and proliferation of hemopoietic stem cel s or exert effects by promoting the adhesion of bone marrow stromal cel s and hemopoietic stem cel s.
OBJECTIVE:To investigate the effects of Radix Astragali injection on the expression of intercel ular adhesion molecule 1 and vascular cel adhesion molecule 1 on the surface of mouse bone marrow stromal cel s.
METHODS:Mouse bone marrow stromal cel s were isolated by adherent culture of whole bone marrow. The morphology of mouse bone marrow stromal cel s were observed using inverted phase microscopy, hematoxylin-eosin staining, optical microscopy and transmission electron microscopy. The optimal concentration at which Radix Astragali injection exhibited the strongest effects on promoting the proliferation of bone marrow stromal cel s was detected using MTT assay. Expression of intercel ular adhesion molecule 1 and vascular cel adhesion molecule 1 on the surface of mouse bone marrow stromal cel s was detected using flow cytrometry after intervention by Radix Astragali injection.
RESULTS AND CONCLUSION:Inverted phase microscopy and optical microscopy showed that bone marrow stromal cel s adhered to the wal of culture flask, exhibited a shuttle-shaped or irregular appearance with processes. Through transmission electron microscopy, organel es were abundant, such as rough endoplasmic reticulum, secretory vesicle, and mitochondria. Radix Astragali injection at 400 and 600 mg/L significantly promoted the proliferation of mouse bone marrow stromal cel s (P<0.05) and there was no significant difference in the cel proliferation between these two concentrations. Radix Astragali injection at 600 mg/L increased the expression of intercel ular adhesion molecule 1 and vascular cel adhesion molecule 1 on the surface of mouse bone marrow stromal cel s. These findings suggest that Radix Astragali injection at appropriate concentrations can increase the surface adhesion of bone marrow stromal cel s, thereby improving hemopoietic microenvironment.