Digital CR of head and neck overcomes the disadvantage of regular X-ray radiography, which can not reveal bone and soft tissue position deficiency in one exposing, and reduces the X-ray radiation dose. Meanwhile, various factors decline the imaging quality, and images must be enhanced in order to meet demands of doctors clinical diagnosis. The general enhancement algorithms don't consider bodys structure difference and density characteristics. A new adaptive CR enhancement algorithms was proposed in this article, and head and neck images were processed with this method and compared with linear unsharp masking method. The results proved that the details of CR image enhanced were abundant and enhanced CR had good visual effect. SNR was high, as well as detail variance/background variance (DV/BV) indicating that this algorithms is suitable for head and neck CR medical image.

Circulating tumor cells (CTCs) are essential for establishing metastasis. Detecting CTCs can help clinicians detect early metastases or recurrences more effectively. Compared with traditional histological analyses and imaging, CTC detection has a much higher reproducibility and sensitivity and can provide more timely prognostic information in human breast cancer.

Objective To investigate the Effect of Tetramethylpyrazine on adhesion and invasion ability of Cell PG in Hypoxic Environment. Methods The revival cell PG was divided into three dosages(high, medium and low)group of Tetramethylpyrazine, DDP group and negative control group. All of five groups were cultured separately in the normal and hypoxic environment. The adhesion ability of Cell PG were detected by MTT. The invasion ability of Cell PG were detected by Transwell chamber. Results Observed on the reconstruction of the basement membrane adhesion in PG cells by MTT, in normoxic environment the mean absorbance of the TMP high, medium and low concentrations, cisplatin group, control group were (0.556±0.040),(0.519±0.056), (0.517±0.061),(0.333±0.057),(0.300±0.066), P<0.01. In hypoxic environment the mean absorbance of the TMP high , medium and low concentrations, cisplatin group, control group were (0.48±0.032),(0.452±0.059),(0.274±0.093),(0.464±0.099),(0.155±0.042), P<0.01. Promotion of cell adhesion with Tetramethylpyrazine of high, medium and low concentration group and DDP group in hypoxic environment is greater than in normoxic environment(P<0.01). Observed the reconstruction of the invasion of basement membrane of PG cells by Transwell chamber, in normoxic environment the invasive cells of the TMP high, medium and low concentrations, cisplatin group, control group were :(28.00±17.00),(91.67±30.37),(105.67±39.58),(85.00±8.19),(152.33±24.71), P<0.05. In hypoxic environment the invasive cells of the TMP high , medium and low concentrations , cisplatin group, control group were (20.33±15.31), (22.33±17.62),(56.00±11.53),(57.67±35.95), (76.33±18.01), P<0.05. The invasive cells of normoxic control group was significantly more than hypoxic control group(P<0.05). Conclusion In hypoxic environment , adhesion and invasion ability of Cell PG significantly decreased compared with normoxic environment ; In normoxia and hypoxia environment, Tetramethylpyrazine and cisplatin could promote the adhesion ability and inhibit the invasion ability of PG cells. Role in promoting of Tetramethylpyrazine on cell adhesion of PG cells was stronger in hypoxic environment.

Objective To explore the feasibility of laparoscopic cholecystectomy(LC) in patients over 85 years old.Methods Three-port LC was performed in 79 patients aged over 85 years old from October 1996 to October 2006.Patients' co-morbidities were effectively controlled before operation.The operation was conducted under continuous epidural anesthesia,and the CO2 pneumoperitoneum pressure was set at 8～10 mm Hg.Results The LC was successfully completed in 70 patients,while conversions to open surgery were required in 9 patients(11.4%).Causes of conversions included severe intraperitoneal adhesion in 6 patients,uncontrolled bleeding in 1 patient,and suspicion of gallbladder carcinoma in 2 patients.Postoperative complications occurred in 10 patients(12.7%,10/79).Of them,1 patient died of acute myocardial infarction,and the remaining 9 patients were cured(including left heart failure in 1 patient,pulmonary infection in 2 patients,ileus in 1 patient,wound haematoma in 1 patient,fungal enteritis in 1 patient,and effusion in the gallbladder bed in 3 patients).Conclusions Proper peri-operative management,effective control of co-morbidities,and careful surgical performance are essential for a safe LC in patients over the age of 85.

Objective To investigate the effect of two kinds of permeation enhancers,azone and clove bud oil,on transdermal permeation of Shuxiong cataplasm. Methods Cataplasm containing azone and clove bud oil,at different concentrations were prepared,with ferulic acid as the index,the role of enhancing permeation of azone and clove bud oil for the main active component were studied by in vitro permeation experiments. Results Both azone and clove bud oil at different concentrations could increase permeation. Clove bud oil was better than azone and the best concentration of Clove bud oil is 3%. Meanwhile,the permeation enhancing multiple of clove bud oil group was the highest which was 3.68. Conclusion With the cumulative permeation quantity of ferulic acid as the index,3% clove bud oil is more suitable to act as a permeation enhancer of Shuxiong cataplasm.

A main complication of chemotherapy in cancer patients is hematopoiesis suppression. Microenviroment transplantation using bone marrow stromal cells (BMSCs) has been demonstrated to be a potent method in recovery of hematopoiesis in animal models. Based on hematopoiesis-supportive ability of BMSCs and high potency of IL-3 in hematopoiesis stimulation, BMSCs were studied as a cellular delivery system for IL-3 gene transfection to promote hematopoiesis recovery of mice after high dose chemotherapy. BMSCs were transfected with recombinant adenovirous containing murine IL-3 gene(MOI = 10), the level of mIL-3 secreted by gene-modified BMSCs was 110U/ml/10~6 cells/ 24h in vitro. The mice were injected with high dose cyclophosphamide(200mg/kg) i.p. and after 24 hours the IL-3 gene-modified BMSCs(2 x 10~6/mouse) were transplanted intrasplenically. White blood cell counts in peripheral blood of mice received intrasplenic injection of IL-3-BMSCs were kept at a high level within two weeks after chemotherapy. The pathological sections of spleens and bone marrow showed significant recovery of hematopoiesis, compared with that of mice received chemotherapy only. The data indicated the feasibility of IL-3 gene-modified BMSCs transplantation in the acceleration of hematopoiesis recovery after chemotherapy.

Dendritic cells (DC) are important antigen-presenting cells in the development of anti-leukemic T-cells responses and it' s reported that DC can differentiate from monocytes or granulocytes in vitro. In the present study, the human leukemic DC were generated from acute promyelocytic leukemic( APL)cells after treatment with cytokines. M3 APL cells were isolated from peripheral blood of patients and incubated with rhGM-CSF( 100ng/ml)or rhGM-CSF( lOOng/ml) plus rhIL-4(500U/ml)for 14 days and the cells were co-cultured wirh TNF-?(100ng/ml)during last 3 days. The results demonstrated that both proliferation and differentiation of APL cells were induced by GM-CSF as the number of cultured cells increased and the cells expressed high level of CD45. Some cells displayed the characteristics of monocytes which expressed CD14 and some were leukemic DC as they expressed GDI a. The co-culturation of APL cells with GM-CSF and TNF-? augmented the differentiation of cells and about 35 percent of leukemic DC generated (was obtained). The maturation of APL cells could also be induced by GM-CSF plus IL-4 but there were few monocytes generated and about 10 percent of the cells were leukemic DC. If cultured for 3 weeks, the number of leukemic DC increased to 60 percent. The addition of TNF-? could augmented GM-CSF and IL-4 induced maturation of cells significantly and 90 percent of leukemic DC generated. Analysis of the cells with electric microscopy indicated that these leukemic DC displayed the similarly morphologic characteristics as monocyte-derived DC and there were still a few granules in their cytoplasm. The leukemic DC expressed high levels of HLA-DR, B7-1, B7-2 and CD54 molecules and could stimulate allo-T cells to proliferate in vitro. Such kind of leukemic DC might be useful tools for the generation of specific anti-tumor CTL and play important roles in immunotherapy of APL.

Objective: To investigate the effects of DC derived from IL-12-induced erythroleukemia cells on the induction of CTL and protective antituinor immunity. Methods: The cytotoxicity of CTL was assessed by 4h 51 Cr-release assay. HE staining and eleclromicroscopy were used to observe the histological changes after immunized with erythroleukemia-derived DCs. Results: After incubation with naive T cells, the IL-12-induced FBL-3 cells could apparently induce the generation of CTL which exihibit the specific cytotoxicity against wild-type FBL-3 cells in vitro. We further assayed the cytotoxicity of CTL in vivo after immunized with erythroleukemia-derived DCs. CTL cytotoxicity of mice immunized with IL-12-in-duced FBL-3 cells was higher than that of mice immunized with IL-I2-uninduced FBL-3 cells. C57BL/6 mice immunized with IL-12-induced FBL-3 cells could resistant the subsequent rechallenge with the wild-type FBL-3 cells. The tumor growth was efficiently inhibited. Histological observation showed that more inflammatory cells infiltrated into the tumor tissue and necrosis of leukemia cells existed. By transmission electron microscopy, apoptositic phenomena were observed in the tumor of group immunized with IL-12-induced FBL-3 cells. However, these changes were not observed in the group immunized with IL-12-uninduced FBL-3 cells. Conclusion: These data indicate that erythroleukemia cells-derived DCs could induce specific CTL efficiently in vitro and in vivo, and may be used as new vaccine to activate antituinor immune responses.

Objective To observe the effectiveness of dexamethasone combined with azasetron for the prevention of postoperative nausea and vomiting(PONV) in patients after laparoscopic cholecystectomy(LC).Methods A total of 150 ASA(Ⅰ~Ⅱ) patients undergoing an elective LC were randomly divided into three groups with 50 patients in each group: Group D+A was given intravenous dexamethasone 5mg and azasetron 10mg(2ml) at the end of surgery,Group A received intravenous azasetron 10mg(2ml) at the end of surgery,and Group C received normal saline(NS) 2 ml as the control.Episodes of nausea and vomiting were recorded for 24 h following the surgery.Results The incidence of nausea was 4% in the Group D+A(2/50),which was significantly lower than in the Group A(16%,8/50) and the Group C(34%,17/50)(?~2=4.00 and 14.62;P=0.046 and 0.000).The incidence of vomiting was 2% in the Group D+A(1/50),which was significantly lower than in the Group A (14%,(7/50)) and the Group C(32%,16/50)(?~2=4.89 and 15.95;P=0.027 and 0.000).The incidences of nausea and vomiting were significantly lower in the Group A than in the Group C(?~2=4.32 and 4.57;P=0.038 and 0.033).Conclusions Use of a low dose of dexamethasone in combination with azasetron is more effective than azasetron prophylaxis alone for a successful control of PONV after LC.

0.05).The major adverse effects were myelo-suppression,nausea,vomiting,phlebitis and alopecie.There were highest incidence of thrombocytopenia(51.1%) in GP regimen group compared with other groups,especially in the stage of Ⅲ? and Ⅳ?(totally accounting for 24.4%)(P