In the present study, the contents of pluripotential stem cell (CFU-S) and granulopoietic progenitor (CFU-GM) in bone marrow, and morphological characteristics of hematopoietic tissue were tested at different time after injection of human placental lipopolysaccharide or protein-free serum in normal and ?-ray-irradiated mice. Our study showed that both of these placental extracts could not only stimulate hematopoiesis in normal mice but also accelerate hematopoietic reconstitution, and increase the survival of mice exposed to 7.5 Gy. From these results, we consider that administration of these extracts might be useful in accelerating hematopoietic recovery in patients with chemotherapy or radiotherapy induced neutropenia.

The effect of benzoate estradiol on hemopoiesis in irradiated mice was studied withassays of granulocyto-macrophage progenitic cells (CFU-D) and endogenous spleencolonies (CFU-S) in bone marrow and simultaneous determinations of mitotic index andfrequency of chromosomal aberrations of bone marrow cells, after a single intramuscularinjection of benzoate estradiol 7 days before irradiation.in the initial stage postirradiation, no significant difference was found between thr mean values neither the number of CFU-D nor the frequncy of chromosomal aberrations of both groups , whereas on the 3th, 5th and 9th day, the production of CFU-D of the pretrated group was increased markedly than that of non-treated control group. In addition, a significant increase in the mitotic index of bone marrow cells was observed by days 3-5 postirradiation in pretreated group.These observations suggest that benzoate estradiol provoke an enhancement of prol -eferation of residual stem cell , srather than a decrease its radiosensetivity.

Gastric cancer can spread to the liver through hematogenous metastasis,lymphatic metastasis and serosal invasion of primary tumor.The occurrence of gastric cancer with liver metastasis is only second to the occurrence of peritoneal metastasis.Liver metastasis is one of the main distant metastasis of gastric cancer,and is a major cause of cancer-related death.The treatment of gastric cancer with liver metastasis still remains controversial.The optimal treatment strategies should be based on the clinicopathological characteristics of each patients and evidence-based medicine,and the individualized plan should be set through multidisciplinary team discussions.Before the more results of prospective studies released,multidisciplinary treatment is the main method,and the appropriate patients should be cautiously choosed for surgery.

: In recent years, more attention has been attracted by non-invasive measurement of the arterial elasticity function. Pulse wave velocity (PWV) assay is one of the commonly used Methods . The PWV measured on different sites of the artery system indicates the elasticity characteristics of the arterial segment and may have different pathophysiology bases and clinical significance.

BACKGROUND:In the total hip arthroplasty, intravenous and topical tranexamic acid can significantly reduce the perioperative blood loss and blood transfusion rate, but at present, the clinical application of tranexamic acid is stil controversial. OBJECTIVE:Cochrane system evaluation method is applied on the efficacy and safety of intravenous combined with topical application versus single-dose intravenous application of tranexamic acid in the total hip arthroplasty. METHODS:We searched the Cochrane Library, PubMed, Ovid, EMBASE, CBM, Wanfang, VIP and CNKI database, from inception to July 2016 and retrieval of randomized control ed trials concerning intravenous combined with topical application and single dose intravenous application of tranexamic acid during total hip arthroplasty. Total blood loss, intraoperative blood loss, postoperative blood loss, blood transfusion rate, thrombosis, operation time, and length of hospital stay were analyzed by meta-analysis. RESULTS AND CONCLUSION:(1) There were seven randomized control ed trials with 620 patients. (2) Meta-analysis results indicated that total blood loss, intraoperative blood loss, postoperative blood loss, and blood transfusion rate were significantly better in the combination group than in the single dose group (P<0.05). There were no significant differences in thrombosis, operation time, and length of hospital stay between the two methods (P>0.05). (3) These results suggested that compared with the single dose of intravenous application, intravenous combined with topical application, can significantly reduce the loss of blood volume and blood transfusion rate and did not increase the rate of thrombosis. Subject headings:Arthroplasty, Replacement, Hip;Tranexamic Acid;Venous Thrombosis;Hemorrhage;Tissue Engineering

The performance evaluation of science and technology management personnels could stimulate their enthusiasm and advance the development of science.In this article,a 3-dimensional model was set up based on the evaluating criteria,the evaluating objects & the familiarity between the appraiser and the objects,which was simplified from several complicated factors necessary in the process of evaluation.Compared with the traditional methods,this 3D model sheds more light on choosing the critical factors,which helps improve the accuracy and rationality of scientific evaluation.

Objective:To investigate the expressions of P53and nm23 proteins in cancer tissue of patients with oral squamous cell carcinoma.Methods:There were 57 males and 43 females,ranging in age from 35 to 75 years old.The clinical stages were T1 10,T2 29,T3 56 and T4 5;pathologic classifications were high differentiation in 74,medial and low differentiation in 26;lymph node metastasis in 56,no metastasis in 44.The expressions of P53 and nm23 proteins were detected by immunohistochemical technique.Results:The expressions rates of P53and nm23 proteins were 58.0%(58/100) and 40.0%(40/100) respectively (P＜0.05)in cancer tissue of patients with oral squamous cell carcinoma.The expressions rates of P53and nm23 were 71.5%(40/56)and 28.6%(16/56)in group of patients with node lymph node metastasis;40.9%(18/44)and 54.5%(24/44) in group of patients with negative metastasis respectively.Conclusion:Lymph node metastasis might be correlated with high level expression of P53 protein and low level expression of nm23 protein in patients of oral squamous cell carcinoma.

Objective To investigate the value of intermediated cutting approach in the total thyroidectomy.Methods The clinical data of patients with total thyroidectomy from January 2009 to December 2011 were analyzed retrospectively.Patients in group A(n =134) were underwent thyroidectomy through intermediate approach,whereas patients in Group B (n =187) were underwent traditional total thyroidectomy.The analysis index included operation periods,operative bleeding amount,the incidence of hypocalcemia and rate of laryngeal nerve injure.Results The operation periods in Group A was shorter than that in group B ((65.4 ± 8.7) second vs.(90.1 ± 11.3) min,t =22.195,P ＜ 0.01).The amount of operative bleeding in group A was,less than that in group B ((20.5 ± 5.7) ml vs.(50.8 ± 11.4) ml,t =31.294,P ＜ 0.01).The incidence rates of temporary recurrent laryngeal nerve injure and the temporary hypocalcemia rate in group A and group B were (0.74％ vs.5.88％,10.44％ vs.24.59％ respectively,x2 =5.72,10.28 respectively,P ＜ 0.05).No permanent hypoparathyroidism or permanent recurrent laryngeal nerve injure case occurred in both groups.Conclusion Intermediate cutting approach was an effective and safe method in terms of total thyroidectomy.

Objective To evaluate the effectiveness of interventional procedures in the treatment of acute pulmonary embolism(PE). Methods Pulmonary arterial thrombolytic therapy, suction and fragmentation of thromhi, anti-spasm, and balloon dilation were performed in eighteen cases with clinically highly suspected PE confirmed by emergent pulmonary artery angiography beforehand. Wedged pulmonary arterial pressure, pulmonary recanalization rate, the display ratio of distal pulmonary capillary net, blood gas analysis, blood oxygen saturation, and the improvement of clinical symptoms and signs were used for evaluation of the effectiveness. Seventeen of the alive cases with dislodgement of deep vein thrombi of the lower extremities were confirmed for the formation of PE and then thrombolytic treatment of the thrombotic deep vein was performed after the placement of inferior vena cava filter. Results Pulmanory artery angiographies showed embolism of the pulmonary arterial trunks or more than two of the branches. The post-treatment pulmonary patency reached 80%-90% in three cases, 90%-95% in eleven cases and 100% in five cases. The display ratio of distal pulmonary capillary net was over 90% in all the cases. Wedged pulmonary arterial pressure decreased to below 25 mmHg in thirteen cases, and to 25-30 mmHg in five cases. Blood oxygen saturation rate improved immediately to 90%-95% in ten cases and to 95%-100% in eight cases. Cough, hemoptysis, dyspnea, and chest pain were completely relieved in thirteen cases and significantly relieved in five cases. Digestive track bleeding was complicated in one case. Conclusions Pulmonary artery angiography is the golden standard for the diagnosis of pulmonary embolism. Interventional therapy is very effective for acute pulmonary embolism, which can significantly reduce the mortality rate.

Objective To assess the effectiveness and safety and biocompatibility of balloon-expanding stainless steel stents (SSS) covered with polyurethane membrane (PUM), expanding poly teflon ester membrane(ePTFEM)and biomembrane (BM) in the treatment of experimental saccular aneurysms of carotid arteries in canine models.Methods 36 experimental saccular aneurysms of carotid arteries in 20 canines were created successfully by sugery. Experimental aneurysms were treated with covered stents including twelve PUM-SSS, twelve ePTFEM-SSS and twelve BM-SSS for each twelve canines repectively. They were placed endovascularly in the common carotid arteries covering the orifice of aneurysms. Follow up angiography was performed immediately after the procedure and 2, 4 and 12 weeks afterwards under the control of conventional anticoagulation. The animals were then sacrificed for histopathologic and statistical investigation. Results 36 stents were placed successfully in the target arteries. The complete patency rates of PUM-SSS, ePTFEM-SSS and BM-SSS were 25.0%, 41.7% and 91.7% respectively. Histological analysis indicated that all treated aneurysmal pouches were filled with thrombus. Stent wires were found to be located deep within the vessel wall and encased by extension of the tunica intima. Endothelialization of BM-SSS groups already began at the 2nd week which was earlier than that of PUM-SSS and ePTFEM-SSS. Various degrees of degenerative cells were seen under the transmission electron microscopy without surface erosion of stents. Conclusion Placement of covered stent endovascularly is expedient, safe, and effective. BM-SSS provides the best mechanical behavior, physiochemical stability, anticoagulative ability and biocompatibility.