[Objective]To understand non-trauma mechanic ventilation treating acute respiratory failure of organophosphorus pesticide poisoning and observe the effect.[Method]16 cases of organophosphorus poisoning together with respiratory failure were treated with non-trauma mechanic ventilation,then observed clinical effect and blood-gas changes.[Result]15 cases got safe and went out of hospital;1 was opened thachea and died from multi-organic failure.Average machine time was 7.8 hours,before going machine:PaO243.92?7.95mmHg,SaO280.6?5.9%;after ventilation,the disease was stable,the artery blood-gas:PaO283.65?4.72mmHg,SaO296.6?4.1%.There's marked difference between them,no complications happened in treatment.[Conclusion]Mechanic ventilation is a key link in saving severe respiratory failure caused by organophosphorus poisoning.Success or not is closely related with going-machine time,meanwhile,the energy-recovery agent must be used enough and reasonably to quickly reach atropine function,as well as to strengthen trachea nursing,by these can death rate be reduced effectively for severe organophosphorus poisoning.

[Objective] To explore the status of chlorite pollution in drinking water due to chlorine dioxide , aswell as its causes and counter measures. [Methods]A water plant collecting surface water as raw water slightly pol-luted by organic compounds and B water plant collecting ground water without organic compounds pollution wereselected as observed objectives. Chlorine dioxide generators were used in both of A and B water plants, their rawmaterials was chlorite for A plant and chlorate for B plant. The levels of chlorite in treated water from these twowater plants were determined by amperometric titration. [Results]The levels of chlorite in treated water of A waterplant ranged 0.530～0.760 mg/L, 2.6～3.8 times of the standard value, with a over standard rate of 100％, thelevels of B water plant range 0.257～0.733 mg/L, 1.3～3.7 times of the standard value, with a over standard rateof 83.3％. [Conclusion] The treated water of A and B water plants presented higher pollution by chlorite, the by-product of chlorine dioxide disinfection.

Objective To study the effects of ultraviolet(UV)radiation disinfection on purified wecter in water dis-penser.Methods The original structure of water dispenser was modified by installing additional UV lamps.The water samples were collected from the tap of the newly modified water dispenser simulated the practical status of water supply in indoor environment under the different conditions including different intensities of UV lamps and water flow rates.The total counts of bacteria of water samples were determined based on the Sanitary Standard for Bottled Purified Drinking water(GB17324-1998).Results The total counts of bacteria of water samples collected from the tap of the newly mod-ified water dispenser were below20cfu/ml,which was lower than those of control water sample(P

0.05). In HiLo group, the levels of MDA and SOD did not changed signific antly(P

Objective To develop a method for detection of avanafil and flibanserin adulterated in health food by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods The separation and analysis were performed on an Agilent Eclipse Plus C18 column(2.1 mm×100 mm,1.8 μm),with a mobile phase of acetonitrile and 0.1%acetic acid(containing 20 mmol·L-1 ammonium acetate)(60:40).Electrospray ionization(ESI) source was applied and operated in positive mode.Multiple reaction monitoring(MRM) mode was used to quantify avanafil and flibanserin.Results The assay linearity of avanafil and flibanserin were confirmed in the range of 2-20 ng·mL-1(r2>0.99).The extraction recoveries varied from 94.6% to 110.0%,and the precision of RSD was <5.0%.The limits of detection were 0.21 and 0.42 μg·kg-1 for avanafil and flibanserin,respectively.Conclusion The method was specific,sensitive and accurate.Therefore,it can be used to detect avanafil and flibanserin which were illegally added in health food.

Objective To detect the mutations of Von Hippel-Lindau (VHL) gene via analyzing the prevalence of family members of VHL syndrome,clinical diagnosis and treatment,and gene analysis of patients with hemangioblastoma.methods All members of the VHL syndrome family members improved all relevant tests and plotted the family map.5 ml peripheral blood was extracted for gene sequencing,and the sequencing Result s were compared with the reported mutations of VHL gene in NCBI database.Result s(1)Analysis of clinical data of four members of the family:Ⅰ-2,Ⅱ-1,Ⅱ-5 suffering from central nervous system hemangioblastoma, Ⅱ-3 with pancreatic,retinopathy and pheochromocytoma,and Ⅱ-5 also combined with kidney,pancreatic lesions.The second generation of patients in the family have been treated surgically.(2)Gene sequencing Result s showed that all subjects in the test had the same mutation:exon2 109 sequence ATATCACACTGCCA was deleted and termination codon UGA appeared in exon 502.Conclusion Through the mutations of the VHL syndrome family,it is found that the family mutation type is a new mutation.For patients with central nervous system hemangioblastoma-based should be suspected of the disease and improve the family history survey.Once the diagnosis of familial VHL syndrome patients are confirmed,it is necessary to inform the other members of the family for clinical screening,and carry out genetic testing to reduce the harm of the disease to the greatest extent.

Currently,three predominant subtypes of influenza virus are prevalent in pig populations worldwide:H1N1,H3N2,and H1N2.European avian-like H1N1 viruses,which were initially detected in European pig populations in 1979,have been circulating in pigs in eastern China since 2007.In this study,six influenza A viruses were isolated from 60 swine lung samples collected from January to April 2011 in eastern China.Based on whole genome sequencing,molecular characteristics of two isolates were determined.Phylogenetic analysis showed the eight genes of the two isolates were closely related to those of the avian-like H1N1 viruses circulating in pig populations,especially similar to those found in China.Four potential glycosylation sites were observed at positions 13,26,198,277 in the HA1 proteins of the two isolates.Due to the presence of a stop codon at codon 12,the isolates contained truncated PB1-F2 proteins.In this study,the isolates contained 591Q,627E and 701N in the polymerase subunit PB2,which had been shown to be determinants of virulence and host adaptation.The isolates also had a D rather than E at position 92 of the NS1,a marker of mammalian adaptation.Both isolates contained the GPKV motif at the PDZ ligand domain of the 3' end of the NS1,a characteristic marker of the European avian-like swine viruses since about 1999,which is distinct from those of avian,human and classical swine viruses.The M2 proteins of the isolates have the mutation (S31N),a characteristic marker of the European avian-like swine viruses since about 1987,which may confer resistance to amantadine and rimantadine antivirals.Our findings further emphasize the importance of surveillance on the genetic diversity of influenza A viruses in pigs,and raise more concerns about the occurrence of cross-species transmission events.

Objective To evaluate the diagnostic value of detection of IgM antibodies to EV71-infection patients,and compared characterisation of RT-PCR,IgM capture ELISA and neutralization test.Methods Virus RNA,neutralization titer and IgM antibody in 115 EV71-infection patients were detected by EV71 real-time RT-PCR kit( EV71-PCR kit),neutralization test,and EV71 IgM-capture ELISA kit (EV71-IgM kit),respectively.Results Using EV71-IgM kit,the detection rate was 80.9％ (93/115,95％ CI:72.5-87.6) among the 115 EV71-infection patients,and was 2.6％ among the 228 healthy children.Simultaneously,sera collected after 1-2 day of disease onset showed an IgM positivity of 70.4％ (38/ 54).The positive rate of EV71-PCR among these patients was 82.6％ (95/115,95％ CI:74.4-89.0),so there was no statistically significant differences between it and EV71-IgM kit.In addition,the detection rate in EV71-infection patients could increase to 92.2％ by combined detection of EV71-PCR and EV71-IgM kit.Conclusion EV71-IgM kit was a rapid and valuable way for the early diagnosis of EV71 infection,and could significantly improve detection rate for EV71 infection by combining with EV71-PCR kit.

Aim To explore the role of ERO1 αand its DNA methylation in homocysteine (Hcy)-induced in-hibition of hepatocytes proliferation.Methods The hepatocytes stimulated with 0 μmol·L -1 Hcy were set as the normal group (NC group)and the hepatocytes stimulated with 1 00 μmol·L -1 Hcy as the experimen-tal group (Hcy group).Methyl thiazolyl tetrazolium (MTT)reduction assay was used to reflect the prolifer-ation of the hepatocytes;qRT-PCR and Western blot were used to detect the mRNA and protein levels of ERO1 α;the expression of green fluorescence protein was observed in hepatocytes after the recombinant plas-mid of ERO1 α was constructed,which was used to confirm if the recombinant plasmid into hepatocytes was successful,then the mRNA and protein levels of ERO1 αwere assayed and the proliferation of the hepa-tocytes was also detected;ntMSP was used to detect the change of ERO1 αDNA methylation.Results The mRNA and protein levels of ERO1 αwere decreased in Hcy group compared with NC group,and the prolifera-tion activity of hepatocytes in Hcy group was de-creased.Sequencing result showed that the recombi-nant plasmid of ERO1 αwas constructed successfully. QRT-PCR and Western blot revealed that ERO1 αwas overexpressed. The result of MTT suggested that ERO1 αoverexpression restored hepatocyte proliferation inhibited by Hcy.Hcy caused ERO1 αDNA hyperm-ethylation.Conclusions Hcy inhibits hepatocyte pro-liferation by downregulating the expression of ERO1 α, and methylation of ERO1 αpromoter may play a role in this process.

The authors first segment the right ventricle regions from the echocardiographics by introducig a seeded region growth algorithm. Then they reconstrunct the three dimensional data field of right ventricle by using rotary scanning interpolation algorithm. And then, the authors propose a layer-by-layer, row-by-row, and voxel-by-voxel approach based on the interpolation results to estimate the volume of right ventricle. Finally, some right ventricle functional parameters such as EDV (End-diastolic volume), ESV (End-systolic volume), and RVEF (Right ventricular ejection fraction) are computed according to the volume results. The volume variation curves of several patients during a cardiac cycle have been successfully plotted out. The obtained results are compared with those from Tomtec medial image workstation. The comparison indicates the proposed approach is practical and meaningful.
Algorithms ; Echocardiography, Three-Dimensional ; instrumentation ; methods ; Heart Ventricles ; diagnostic imaging ; Humans ; Image Processing, Computer-Assisted ; Ventricular Dysfunction, Right ; diagnostic imaging ; physiopathology ; Ventricular Function, Right