Objective To understand the traffic pollution in main crossroads of Changsha. Methods The levels of noise, NO 2, O 3 and TSP and traffic volumn were monitored in 6 main crossroads of Changsha in situ, 3 times per day, continuously for 5 days. Results The traffic volumns were 1 203～3 715/h, the noise levels were 69～88 dB(A), the levels of NO 2, O 3 and TSP in air of 6 main crossroads were 0.092～0.238 mg/m 3, 0.238～0.303 mg/m 3 and 0.71～1.47 mg/m 3 respectively. The over standard rates were 93%～100% for O 3 with a average rate of 98%. Conclusion The traffic pollution in main crossroads of Changsha mainly induced by noise and O 3, which might be associated with the traffic volumn, the environment around the crossroads and the types of the automobile running in the crossroads.

Objective To study the general toxicity of formaldehyde on germ cells and its effects on the SDH activity in testicle tissue of mice. Methods The 30 male Kunming mice were divided into 5 groups. 3 groups as exposure groups were in-traperitoneally injected with formaldehyde at doses of 0.20, 2.00 and 20.00 mg/kg respectively and continuously for 5 days. The other 2 groups were considered as controls positive and negative group. The pathological changes of testicle tissue of mice were observed by microscopy and the activity of SDH in testicle tissue was determined. The sperm count and the abnormal sperm's heads were observed by microscopy. Results Formaldehyde could induce the degeneration and necrosis in germ cells of mice. With the increases of the exposure doses of formaldehyde, the sperm count and the activity of SDH in testicle tissue of mice decreased (P

Objective To explore the optimal dose-effect relationship of gene transfer according to the expression of pEGFP in tumor cells under different parameters mediated by microbubble contrast agent and ultrasound in vivo.Methods C57BL/6J mice were inoculated subcutaneously in the middle of the right flank with hepal-6 tumor cells.The mixture of SonoVue and enhanced green fluorescent protein(EGFP)plasmid was injected into the tail vein of each mouse, groups were randomly established according to different output intensity( 1 W/cm2, 2 W/cm2, 3 W/cm2 ), exposure time ( 1 min, 5 min, 10 min) and volume of SonoVue(30 μl,60 μl,90 μl), the expression of EGFP in tumor cells under different parameter was examined by flow cytometry and fluorescence microscopy.Results The expression of pEGFP in tumor cells was significantly higher with the increase of exposure time,output intensity and volume of SonoVue (1 min vs 5 min, P＜0.05; 1 W/cm2 vs 2 W/cm2, P＜0.05 ; 30/μl vs 60 μl, P＜0.05).However, the transfection efficiency of EGFP can not be enhanced continuously with the increasing exposure time,output intensity and volume of SonoVue (5 min vs 10 min, P＞0.05; 2 W/cm2 vs 3 W/cm2, P＞0.05; 60 μl vs 90 μl, P＞0.05).The highest transfection effieieney was achieved under intensities was 2 W/cm2 [(21.02±1.45)%]with 5 min[(23.22±1.91)%] exposed to ultrasound, and 60 /μl SonoVue [ (21.02±1.45) % ].Conclusions Gene transfeetion efficiency changded with the difference parameters.2 W/cm2,5 min and 60 μl SonoVue led to the optimum dose-effect relationship of gene transfer in vivo .

Hepatic Veins ; physiopathology ; Humans ; Hypertension, Portal ; physiopathology ; Portal Pressure

Arteriovenous Fistula ; Humans ; Hypertension, Portal

The performance of transjugular intrahepatic portosystemic shunt (TIPS) has two key procedures: (1) portal vein branch puncturing, and (2) the correct judgment of the safety of the puncture site. The portal vein branch puncturing is the most important and difficult step for a successful TIPS procedure. Therefore, to find and to establish an proper access to the portal vein is critical. Nowadays, in clinical practice several imaging techniques have been used to localize the portal vein, such as magnetic resonance imaging, sonography, fluoroscopy, arteriography and computed tomography. This article aims to make a general review on these invasive and non - invasive localization techniques when a successful performance of TIPS is expected.

OBJECTIVETo evaluate the agreement and correlation between hepatic vein pressure gradient (HVPG) and portal vein pressure (PVP) in patients with portal hypertension,and explore their clinical value.

METHODSA total of 46 patients with portal hypertension were directly measured the free hepatic pressure, wedged hepatic pressure, portal vein pressure before and after TIPS therapy. The agreement and correlation of HVPG and PVP were analyzed, and explore their clinical value.

RESULTSThere is no significant agreement or correlation between HVPG and PVP in 5 patients, whose third hilar have large communicating branches between portal vein and Inferior vena cava, or with obvious umbilical vein opened. The HVPGs were significantly agreed with portal vein pressure in other 41 patients. There is no significant difference of HVPG or PVP between earlyTIPS and not early-TIPS groups. In addition, the portal vein pressures after TIPS were significantly decreased compared with that before TIPS.

CONCLUSIONThe HVPG can well show the PVP except these with obvious communicating branches between portal vein and Inferior vena cava in third hilar, and TIPS can effectively decrease the portal vein pressure in patients with portal hypertension.

OBJECTIVE: To investigate the function of bone marrow mesenchymal stem cells (BMSCs) with over-expressed matrix metalloproteinase 1 (MMP1) on liver fibrosis. METHODS: Fifty SD male rats were randomly divided into 4 groups: recombinant adenovirus Adhuman MMP-1(hMMP-1)-enhanced green fluorescent protein (EGFP) transfected BMSCs group (Group A, n=10), Ad-EGFP transfected BMSCs group (Group B, n=10), liver fibrosis group (Group C, n=15), and a normal group (Group D, n=15). The liver fibrosis model was formed by subcutaneous injection of the mixed liquor of carbon tetrachloride (CCL4) and vegetable oil. After 10 weeks, the model of liver fibrosis was formed. Group A and B were administered the transfected BMSCs via the tail veins, while Group C and D were administered normal saline. After 3 weeks, the rats were sacrificed. The body weight, liver weight, liver function, liver fibrosis indexes and liver pathological changes were tested. RESULTS: Compared with the control group, the rats administered BMSCs with over-expressed MMP1 showed a significant improvement in the body weight, liver weight and plasma albumin (ALB) (P<0.05), and a significant reduction in the plasma alanine aminotransferase, total bilirubin, hyaluronic acid, laminin and procollagen III (P<0.05). Hematoxylin-eosin staining confirmed that the degree of liver fibrosis was significantly ameliorated under average visual fields (P<0.05). CONCLUSION The repair ability of BMSCs on liver fibrosis can be enhanced by over-expression of hMMP-1.
Adenoviridae ; Animals ; Carbon Tetrachloride ; Green Fluorescent Proteins ; Hematopoietic Stem Cells ; cytology ; Liver Cirrhosis ; chemically induced ; therapy ; Male ; Matrix Metalloproteinase 1 ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Transfection