Objective To study the safety and effect of ultrasound guided minimally invasive rotary cutting for treatment of benign breast lesions. Methods A single-center, prospective, randomized, controlled study method was used, 84 patients with breast benign lesions were randomly divided into observation group and control group, 42 patients in each group. Patients in the observation group were treated with ultrasound guided minimally invasive rotary cutting, patients in control group were treated with traditional surgery. The intraoperative and postoperative related indicators were compared between two groups. Results The intraoperative blood loss, length of incision, operation time in breast benign unilateral single and unilateral and bilateral lesions in the observation group were significantly less than those in the control group, with statistically significant difference (P<0.01) . The healing time, postoperative pain score, complications in breast benign unilateral single and unilateral and bilateral lesions in observation group were less than those in the control group, with statistically significant difference (P<0.01) . And the postoperative satisfaction, and the excellent rate of beauty in breast benign unilateral single、unilateral and bilateral lesions were better than control group, with statistically significant difference (P<0.01) . There was no breast deformation in observation group after treatment, and there were 3 patients of breast deformation in the control group has 3 patients, accounting for 7.14%. Conclusion Ultrasound guided minimally invasive rotary cutting is significantly superior to the traditional treatment in security and effectiveness for treatment of breast benign lesions in one single and unilateral multiple and bilateral lesions.

Objective To investigate the effects of estrogen on extracellular signal-regulated kinase-1/2(ERK1/2) phosphorylation levels in hippocampal CA4 region of ovariectomized rats. Methods Experimental female Wistar rats were randomly divided into three groups: intact (INT) group, ovariectomized (OVX) group, and OVX+estrogen (1 mg/kg, weekly) group. Radioimmunoassay (RIA) was used to measure the estrogen content in plasma, and the levels of ERK1/2 phosphorylation were measured with immunohistochemistry. Results Compared with the control animals, the plasma estrogen level was significantly lower in OVX group (P＜0.001). However, the plasma estrogen level was higher in the OVX+estrogen group than that of the OVX group (P＜0.001). Immunohistochemical staining showed that the phospho-ERK1/2 (p-ERK1/2) substances were present in the nucleus and cytosol of neurons. Compared with the control group, immunostaining positive cells markedly decreased in OVX group, with light nucleus and cytosol staining in the hippocampal CA4 region (P＜0.001). The phospho-ERK1/2 positive neurons increased more obviously in OVX+estrogen group than in the OVX group (P＜0.001). Conclusion Estrogen can increase ERK1/2 phosphorylation levels in hippocampi of aging female rats, which suggests that the ERK signal transduction pathway may be regulated by estrogen during the process of aging.

Objective To determine the regulation of electroacupuncture(EA)at Zusanli acupoint for the expression of estrogen receptors mRNA in the brain of ovariectomized rats.MethodsFemale Wistar rats were randomly divided into 3 groups:intact group(INT),ovariectomized group(OVX),and ovariectomy and acupuncture group(OVX+EA).Radioimmunoassay(RIA)was used to measure the estradiol(E2)and testosterone(T)levels in plasma.The mRNA levels of estrogen receptor(ER)α and ERβ in the brain of rats were measured with RT-PCR.ResultsThe levels of endogenous estrogen increased significantly in OVX+EA rats compared with OVX rats(P<0.01),while the plasma T levels were lower in the OVX+EA group than those of the OVX group(P<0.01).Eelectroacupuncture can up-regulate ERα expression in the brain of ovariectomized rats(P<0.01).However,it was shown to have a down-regulatory effect on the levels of ERβ expression(P<0.01).ConclusionThere is an intimate relationship between the expressions of estrogen receptors mRNA and the endogenous hormone levels,such as E2 and T.Electroacupuncture can regulate the expression of estrogen receptors mRNA in the brain and the E2 and T levels in plasma of ovariectomized rats,which might,at least in part,explain the mechanisms of acupuncture adjusting the neuroendocrine system.

Objective To study the radiographic findings of the chest in patients after open heart surgery and its evaluating methods.Methods 236 cases after open heart surgery were divided into 3 groups according to the date of the first postoperative chest films:0～3 d,4～7 d and 8 d or more.The findings on pre and postoperative chest films were compared in 7 aspects respectively.Results 8 typses of complications were detected including severe ones such as acute pulmonary edema,the low cardiac output syndrome and ARDS.The incidences of widening of the mediastinum,pleural effusion and pulmonary interstitial edema were lower in over 8 d group than those in the other two groups(?

Objective To investigate the effects of estrogen on brain derived neurotrophic factor (BDNF) and neuropeptide Y (NPY) levels in cerebellar cortex of ovariectomized rats. Methods 24 female Wistar rats were randomly divided into three groups: intact (INT) group, ovariectomized (OVX) group, and OVX+estrogen 0.5 mg/kg every day group (E group). Radioimmunoassay (RIA) was used to measure the estrogen content in plasma, and the levels of BDNF and NPY were measured with Immunohistochemistry. Results Compared with the INT group, the plasma estrogen level significantly reduced in OVX group (P<0.001). However, the plasma estrogen level was higher in the E group than that in the OVX group (P<0.001). The BDNF and NPY presented in the Purkinje cell layer,and BDNF also distributed in the molecular layer and granular layer. Compared with that in the INT group, BDNF and NPY positive cells markedly decreased in OVX group, with slight cytosol staining in the cerebellar cortex (P<0.001). The BDNF and NPY positive neurons increased in E group compared with that in the OVX group (P<0.001). Conclusion Estrogen can increase the BDNF and NPY levels in cerebellar cortex of female rats, which may protect the structure and function of cerebellar neurons.

Objective To develop fast detection techniques for the diagnosis of gonococcal infections.Methods Prokaryotic expression vector for Neisserial surface protein A (NspA) was constructed using the NspA gene cloned by PCR.Mice were immunized with the renatured recombinant NspA (rrNspA)to prepare antibodies against NspA.Western blot and ELISA was used to analyze the binding of NspA antibodies to lysate of gonococcal cells or to intact gonococci.Results NspA antibodies that were prepared by the rrNspA expressed in E.coli could bind to rrNspA,the natural NspA existing in gonococcal cells,or intact gonococci.Conclusion RrNspA and its antibodies have potential value in developing fast diagnostic kits for gonococcal diseases.

Objective To assess the prevalence of depressive symptoms,trends on its longitudinal development and related influencing factors among primary and middle school students in Changfeng county of Anhui province. Methods Through cluster sampling methods,all students from 3 to 9 grades in 5 primary schools and 3 middle schools in Changfeng county of Anhui province were investigated through questionnaire as subjects of baseline survey in December 2009. Subjects of this longitudinal study were students in grades 3,4 and 7 at the time of the baseline survey and were contacted once a year for two years. At last,a total of 816 students participated in all the three surveys. A structured questionnaire including Children’s Depression Inventory,socio-demographic characteristics and some potential influential factors was employed for this study. A Generalized Estimating Equation (GEE) was used to evaluate the related influencing factors of children’s depressive symptoms in the longitudinal study. Results Prevalence rates of depressive symptoms were 11.3%,10.4% and 8.5%,respectively,at the baseline,1-year and 2-year follow-up studies, among primary and middle school students. Scores on depressive symptoms of total subjects and children who had always been left at home in the follow-up process showed significant differences at the three surveys (F=13.423,P<0.001;F=5.761,P=0.003). 25 percent of the 92 students who showed depressive symptoms at the baseline survey remained those depressive symptoms at the 1-year follow-up study and 87 percent of the 23 students who had depressive symptoms at the baseline and 1-year follow-up surveys still showing depressive symptoms at the 2-year follow-up study. Results from the GEE program indicated that grade 3 students,having syblings,family with dysfunction or at low level of self-esteem etc.,were prone to development while sex and parents’ educational level were not correlated with depressive symptoms. Conclusion Prevalence of depressive symptoms among primary and middle school students in Changfeng county of Anhui province appeared a decreasing tendency during the follow-up process in our study. Through the longitudinal development,we noticed that the prevalence of depressive symptoms seemed to be related to the personal characteristics,education and family environment of the subjects under our study. Improving the level of family function as well as individual’s self-esteem might positively contribute to mental health of those primary and middle school students.

OBJECTIVETo assess the prevalence of depressive symptoms, trends on its longitudinal development and related influencing factors among primary and middle school students in Changfeng county of Anhui province.

METHODSThrough cluster sampling methods, all students from 3 to 9 grades in 5 primary schools and 3 middle schools in Changfeng county of Anhui province were investigated through questionnaire as subjects of baseline survey in December 2009. Subjects of this longitudinal study were students in grades 3, 4 and 7 at the time of the baseline survey and were contacted once a year for two years. At last, a total of 816 students participated in all the three surveys. A structured questionnaire including Children's Depression Inventory, socio-demographic characteristics and some potential influential factors was employed for this study. A Generalized Estimating Equation (GEE) was used to evaluate the related influencing factors of children's depressive symptoms in the longitudinal study.

RESULTSPrevalence rates of depressive symptoms were 11.3% , 10.4% and 8.5% , respectively, at the baseline, 1-year and 2-year follow-up studies, among primary and middle school students. Scores on depressive symptoms of total subjects and children who had always been left at home in the follow-up process showed significant differences at the three surveys (F = 13.423, P < 0.001; F = 5.761, P = 0.003). 25 percent of the 92 students who showed depressive symptoms at the baseline survey remained those depressive symptoms at the 1-year follow-up study and 87 percent of the 23 students who had depressive symptoms at the baseline and 1-year follow-up surveys still showing depressive symptoms at the 2-year follow-up study. Results from the GEE program indicated that grade 3 students, having syblings, family with dysfunction or at low level of self-esteem etc., were prone to development while sex and parents' educational level were not correlated with depressive symptoms.

CONCLUSIONPrevalence of depressive symptoms among primary and middle school students in Changfeng county of Anhui province appeared a decreasing tendency during the follow-up process in our study. Through the longitudinal development, we noticed that the prevalence of depressive symptoms seemed to be related to the personal characteristics, education and family environment of the subjects under our study. Improving the level of family function as well as individual's self-esteem might positively contribute to mental health of those primary and middle school students.

Adolescent ; Child ; China ; epidemiology ; Depression ; epidemiology ; Female ; Follow-Up Studies ; Humans ; Longitudinal Studies ; Male ; Students ; psychology