Objective To explore the expressions of endothelial growth factor-D (VEGF-D) and urokinase-type plasminogen activator (uPA) in colon carcinoma and to reveal their correlation with the tumor invasion and metastasis. Methods The expressions of VEGF-D and uPA were detected by immunohistochemistry and Western blotting in 30 specimens of colon carcinoma and 30 specimens of normal neighbouring colon tissue 5 cm away from the lesions. Results The expressions of VEGF-D and uPA mostly located in endochylema, less in nucleus, of both tumor tissue and normal tissue, and they were significantly higher in tumor tissue than normal tissue (P

Objective To explore the expression of endothelial growth factor-D (VEGF-D) and uroki-nase-type plasminogen activator(uPA) in rectal carcinoma and to reveal their correlation to the tumor invasion and metastasis.Methods The expression of VEGF-D and uPA in 30 cases with rectal carcinoma and normal tissue was detected by immunohistochemistry and Western blot.Results The expression of VEGF-D and uPA was de-tected both in tumor tissue and normal tissue, but significantly higher in tumor tissue (P < 0.01), they expressed mostly in endochylema.The expression of VEGF-D or uPA was significantly higher in Dukes' stage C + D than that in Dukes' stage A + B(P <0.01), was also higher in tissues with lymph node metastasis or distant metasta-sis than those without metastasis(P <0.05 ,P <0.01).They were found to be lower in cancer tissue along with the differentiation degree increase(P < 0.05) .Condnsions The overexpression of VEGF-D and uPA in rectal carcinoma may play an important part in the tumorigenesis and progression of rectal carcinoma.

Pituitary hyperplasia may be found in patients with primary hypothyroidism as the decreased thyroid hormone level attenuates negative feedback effect.Sometimes the enlarged pituitary may be misdiagnosed as a pituitary tumor.Patients with long term untreated hypothyroidism often have extremely high level of serum creatine kinase and thus may be misdiagnosed as suffering from myositis.In order to increase the awareness of the nonspecific symptoms of primary hypothyroidism,this article introduces the diagnosis and treatment of a patient with primary hypothyroidism with raised serum creatine kinase level and pituitary hyperplasia.

In patient with primary biliary cirrhosis,the metabolism of calcium and vitamin D could be affected and osteomalacia and secondary hyperparathyroidism might occurr.Besides,hypoalbuminemia may mask the real level of serum calcium and thus lead to misdiagnosis of coexisting parathyroid adenoma.Therefore,a rare case of parathyroid adenoma associated with primary biliary cirrhosis was herewith presented to call attention to the effect of hypoalbuminemia on serum calcium.

Objective To observe effects of Genistein and 5fluorouracil(5-FU) on human colon carcinoma cell line Colo320.Methods The MTT assay and median-effect principle were used.Results The two drugs were antagonistic at higher concentrations and synergistic at lower concentrations,The sequence and time of drug administration can influence the effects of the two drugs on Colo320.Conclusion The two drugs were cooperated at lower concentrations and antagonistic at higher concentrations.The sequence and time of drug administration were also important for their effects on the cells.

Parathyroid adenoma is the main cause of primary hyperparathyroidism and often associated with thyroid nodular goiter.Thyrothymic thyroid rest belong to the ectopic thyroids which are classified into 4 grades according to the state of their connection with the proper neck thyroid gland.Thyrothymic thyroid remnant can also develop into nodular goiter and may be difficult to be distinguished from parathyroid adenoma.We present herewith the diagnosis and treatment of a rare case of parathyroid adenoma accompanied by thyrothymic thyroid remnant nodular goiter in order to remind clinicians of the attention to the thyrothymic thyroid remnant disease.

This study was conducted to potentiate the expression of outer membrane protein OmpL17 of the strong virulent L. interrogans serovar Lai and investigate its immunogenicity in rabbits. The OmpL17 was cloned into prokaryotic expression vector pGEX-1lambdaT. The recombination expression plasmid pGEX-OmpL17 was transformed into E. Coli JM109. The GST fused protein GST-OmpL17 was expressed after induction by IPTG, then GST-tag was by thrombin and purified using Bulk GST purification Modules. SDS-PAGE and Western blotting analysis indicated that the molecular weight of GST-OmpL17 and OmpL17 was about 54 KDa and 28 KDa respectively. The outer membrane protein OmpL17 was subcutaneously injected into rabbits and high titre anti-OmpL17 antibody was obtained (1:4896) which could conjugate specifical with OmpL17. In conclusion, OmpL17 and specifical anti-OmpL17 antibody were obtained, which provided an experimental basis for researching pathogenic effect and immunity functions of OmpL17.
Bacterial Outer Membrane Proteins ; biosynthesis ; genetics ; immunology ; Bacterial Proteins ; biosynthesis ; genetics ; immunology ; Cloning, Molecular ; Humans ; Leptospira interrogans ; genetics ; immunology ; Porins ; biosynthesis ; genetics ; immunology ; Recombinant Proteins ; biosynthesis ; genetics ; immunology ; Virulence

OBJECTIVETo observe the signal transducers and activator of transcriptions (STATs) protein expression changes and investigate the functional role of STATs pathway in case of high glucose-induced cardiac fibroblasts (CFs) proliferation and collagen deposition in vitro.

METHODSRat cardiac fibroblasts were isolated from 1- to 3-day-old SD rats, cells from the second to fourth passages were used for the experiment. CFs were cultured in Dulbecco's modified Eagle's medium, supplemented with 5.5 mmol/L glucose (NG), 5.5 mmol/L glucose plus 19.4 mmol/L mannose (OC) or 25 mmol/L glucose (HG) in the presence of absence of STAT1 inhibitor (fludarabine, FLU) and STAT3 inhibitor (S3I-201). After 24 h and 48 h culture in vitro, the proliferation of CFs was measured by 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. After 12 h and 24 h culture in vitro, the production of type I and III collagen was evaluated using real-time quantitative PCR and ELISA. After 0, 30, 60 and 120 min culture in vitro, the phosphorylated expression of STAT1 and STAT3 was analyzed by Western blot.

RESULTSCFs proliferation was significantly enhanced post 24 h and 48 h HG stimulation, and procollagen I and III mRNA expression was significantly upregulated post 12 h and 24 h HG stimulation. Deposition of collagen I and III was also significantly increased post 24 h and 72 h HG stimulation. STAT1 phosphorylation in CFs was increased after 120 min HG stimulation and STAT3 phosphorylation in CFs was increased post 60 min and 120 min HG stimulation. FLU and S3I-201 could inhibit HG-induced CFs proliferation and suppress of which was stimulated by FLU and S3I-201 could both suppress upregulated procollagen I and III mRNA expression and the deposition of collagen types I and III post HG stimulation. STAT1 phosphorylation inhibition resulted in less mRNA downregulation of procollagen type III than that of procollagen type I post 12 h HG stimulation. The STAT3 phosphorylation inhibition resulted in more significantly upregulated procollagen type III mRNA expression than procollagen type I mRNA expression at 12 h post HG stimulation.

CONCLUSIONHG could enhance the protein expression of phosphorylated STAT1 and STAT3 in CFs, which are responsible for HG-induced increased CFs proliferation and collagen deposition in vitro.

Aminosalicylic Acids ; pharmacology ; Animals ; Benzenesulfonates ; pharmacology ; Cell Proliferation ; Cells, Cultured ; Collagen Type I ; metabolism ; Collagen Type III ; metabolism ; Fibroblasts ; cytology ; drug effects ; Glucose ; adverse effects ; Myocardium ; cytology ; Phosphorylation ; RNA, Messenger ; metabolism ; Rats ; Rats, Sprague-Dawley ; STAT1 Transcription Factor ; metabolism ; STAT3 Transcription Factor ; metabolism ; Signal Transduction ; Up-Regulation ; Vidarabine ; analogs & derivatives ; pharmacology