Doxorubicin as a member of the anthracycline-based drugs, is widely used in cancer chemotherapy, while its significant cardiac toxicity and cytotoxicity limit its application to some extent.Therefore, it's a valuable exploring topic on how to improve the effect and reduce the adverse drug reactions of doxorubicin chemotherapy.This paper attempted to analyze the literatures about TCM combined with doxorubicin in nearly 15 years, including clinical use and experimental studies, to provide reference for the clinical application.

Objective To investigate the changes of drug resistance and class Ⅰ integron in clinically isolated Pseudomonas aeruginosa(PA) in our hospital form January 2013 to December 2015.Methods Clinically isolated PA strains were divided into the 3 time periods of 2013,2014 and 2015.Their resistance to 22 commonly used antibacterial drugs was investigated by adopting the VITEK-2;200 strains were randomly selected from the isolated strains during these 3 time periods.Class Ⅰ integron was detect by PCR.Results The detected PA in these 3 time periods had 366,437 and 520 strains respectively.The drug resistance of Pseudomonas aeruginosa to 22 commonly used antibacterial drugs was remarkably increased(P<0.05),especially in ICU.The detection rate of class Ⅰ integron positive bacteria was gradually increased year by year,moreover the drug resistance rate of class Ⅰ integron positive bacteria was significantly higher than that of class Ⅰ integron negative bacteria (P<0.01).Conclusion The drug resistance rate of PA in this hospital is higher.The proportions of multi-drug resistance and classⅠ integron are significantly increased.The hospital infection detection and drug-resistant bacterial monitoring should be strengthened to further standardize the use of antibacterial drugs.

The full length cDNA encoding peroxisomal membrane protein from Chaetomium globosum was cloned using RACE technology and the sequence in cDNA library of C. globosum in GenBank ( Accn: BP099709). The 747bp full length cDNA encoding peroxisomal membrane protein allergen (pero) gene was assembled with 412bp 3'and 508bp 5'RACE products. The open reading frame was 501 bp encoding 166 amino acids. The molecular weight of the protein was 17. 5kDa and its theoretical isoelectric point was 5.75. The pero gene was amplified using specific primers of cDNA 5'and 3'untranslated region, sequence analysis indicated that the gene have 3 exons and 2 introns. ClustalX analysis revealed that amino acids sequence of pero gene from C. globosum and Neurospora crassa shared 83% high similarity. To construct pET28a-pero expressive plasmid, pero gene was inserted into pET28a expressive vector. Escherichia coli BL21 transformed by pET28a-pero plasmid was induced with IPTG. The protein expression was analyzed with SDS-PAGE. A 21kDa pero fusion protein representing the pero gene was expressed in recombinant E. coli BL21. The sequences of cDNA,DNA and deduced amino acid of the pero gene from C. globosum were submitted to GenBank (Accn: AY555771, AY584753,AAS66898).

Child ; Guidelines as Topic ; Humans ; Pneumonia, Pneumocystis ; diagnosis ; therapy

Adult ; Bone Neoplasms ; pathology ; surgery ; Femur ; pathology ; surgery ; Humans ; Male ; Osteochondroma ; pathology ; surgery ; Young Adult

Cochlea ; metabolism ; Deafness ; genetics ; Humans ; Mutation

Objective To observe the clinical effect of bicyclol combined with polyene phosphatidyl choline in elderly non-alcoholic fatty liver disease (NAFLD).Methods One hundred and twenty elderly patients with NAFLD were divided into 3 groups by block randomization method,40 cases in each group.Therapeutic group was treated by bicyclol combined with polyene phosphatidyl choline; bicyclol group was only treated by bicyclol; and polyene phosphatidyl choline group was only treated by polyene phosphatidyl choline.The blood biochemical indexes,liver ultrasound score and clinical curative effect of 3 groups were compared after treated for 24 weeks.Results The total cholesterol (TC),triglyceride (TG),alanine aminotransferase (ALT),aspartate aminotransferase (AST) and gamma glutamine transferase (GGT) in 3 groups after treatment were lower than those before treatment,and the difference was statistically significant (P ＜ 0.05) ; TC,TG and ALT levels in therapeutic group after treatment were significantly lower than those in bicyclol group and polyene phosphatidyl choline group [(1.36 ± 0.84) mmol/L vs.(2.77 ± 1.27),(2.84 ±1.35) mmol/L; (1.32 ±0.71) mmol/L vs.(1.89 ±0.87),(1.92 ±0.90) mmol/L; (38.26 ± 12.75) U/L vs.(57.83 ± 16.67),(62.07 ± 18.16) U/L],and the difference was statistically significant (P ＜ 0.05).The liver ultrasound score in 3 groups after treatment was significantly decreased compared with that before treatment,and the difference was statistically significant (P ＜ 0.05).Liver ultrasound scores in therapeutic group after treatment were significantly lower than those in bicyclol group and polyene phosphatidyl choline group [(2.08 ± 0.93) scores vs.(3.17 ± 1.14),(3.34 ± 1.07) scores],and the difference was statistically significant (P ＜ 0.05).The total effective rate in therapeutic group was significantly higher than that in bicyclol group and polyene phosphatidyl choline group [85.0％ (34/40) vs.67.5％ (27/40),65.0％ (26/40)],and the difference was statistically significant (P ＜0.05).Conclusions Bicyclol combined with polyene phosphatidyl choline has better clinical effect in elderly patients with NAFLD.It is better than single bicyclol and polyene phosphatidyl choline and worth clinical promotion.

Objective To investigate the risk factors related to mortality of children with deep fungal infection in pediatric intensive care unit(PICU).Methods A retrospective case-control study was applied.Ninty-six patients admitted to PICU with clinical or definite diagnosis of deep fungal infection from Nov 2005 to Mar 2009 were included.The risk factors related to mortality wereanalyzed with the logistic regression analysis.The research factors included:sex,age,primary diseases,complications,invasive operations and therapeutic measures etc.Results Of all 96 children,28 died (28.2％).According to the analytical results of multivariate logistic regression,the variables significantly associated with mortality were immunosuppressive (OR =185.770,95 ％ CI 11.467 ～ 3 009.507),mechanical ventilation (OR =11.555,95 ％ CI 2.780 ～ 48.039),hypoproteinemia (OR =1.246,95％ CI 1.133 ～ 1.369) and low pediatric critical illness score (OR =1.086,95 ％ CI 1.008 ～ 1.169).Conclusion The risk factors related to mortality of children with deep fungal infection in PICU were immunosuppressive,mechanical ventilation,hypoproteinemia and low pediatric critical illness score.

Objective To characterize the clinical presentation of noncardiac chest pain(NCCP), to investigate the quality of life among NCCP patients,and to understand the relation between NCCP and acid reflux and the value of proton pump inhibitor(PPI) in diagnosis of NCCP.Methods Eighty-seven NCCP patients were enrolled to the study.All patients received lansoprzole 30 mg twice daily for 7 days. Patients filled out a questionnaire survey before and after the PPI treatment.The questionnaire included the general personal data,the symptom scale,the medical outcome study 36-item short form healthy survey(SF-36),Zung Self Rating Anxiety Scale(SAS) and Zung Self-rating Depression Scale(SDS).The results were analyzed in comparisons of healthy control group.Results①The most common cause for NCCP was gastroesophageal reflux disease (GERD).The typical reflux syndrome presented among 34.5% of the patients.②Among 87 patients,acid-related NCCP was accounted for 56.3% (49/87), whereas non-acid-related NCCP was accounted for 43.7% (38/87).The sensitivity and specificity of PPI test were 91% and 81%,respectively.③Except the role of role emotional(RE),7 healthy conceptions in the health-related quality of life in NCCP patients were significantly lower than those in healthy volun- teers(P